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Theor Appl Genet (2008) 118:1527 DOI 10.1007/s00122-008-0873-5
ORIGINAL PAPER
BAC-derived markers converted from RFLP linked to Phytophthora capsici resistance in pepper (Capsicum annuum L.)
Hyoun-Joung Kim Seok-Hyeon Nahm Heung-Ryul Lee Gi-Bo Yoon Ki-Taek Kim Byoung-Cheorl Kang Doil Choi Oh Yeol Kweon Myeong-Cheoul Cho
Jin-Kyung Kwon Jung-Heon Han Jeong-Ho Kim MinKyu Park Jong Hwa Ahn Soon Ho Choi Nam Han Her Joo-Hee Sung Byung-Dong Kim
Received: 22 February 2008 / Accepted: 15 August 2008 / Published online: 16 September 2008 Springer-Verlag 2008
Abstract Phytophthora capsici Leonian, an oomycete pathogen, is a serious problem in pepper worldwide. Its resistance in pepper is controlled by quantitative trait loci (QTL). To detect QTL associated with P. capsici resistance, a molecular linkage map was constructed using 100 F2 individuals from a cross between Capsicum annuum CM334 and C. annuum Chilsungcho. This linkage map consisted of 202 restriction fragment length polymorphisms (RFLPs), 6 WRKYs and 1 simple sequence repeat (SSR) covering 1482.3 cM, with an average interval marker distance of 7.09 cM. QTL mapping of Phytophthora root rot and damping-oV resistance was performed in F2:3 originated from a cross between resistant Mexican landrace
C. annuum CM334 and susceptible Korean landraceC. annuum Chilsungcho using composite interval mapping (CIM) analysis. Four QTL explained 66.3% of the total
phenotypic variations for root rot resistance and three44.9% for damping-oV resistance. Of these QTL loci, two were located close to RFLP markers CDI25 on chromo-some 5 (P5) and CT211A on P9. A bacterial artiWcial chromosome (BAC) library from C. annuum CM334 was screened with these two RFLP probes to obtain sequence information around the RFLP marker loci for development of PCR-based markers. CDI25 and CT211 probes identiWed seven and eight BAC clones, respectively. Nine positive BAC clones containing probe regions were sequenced and used for cytogenetic analysis. One single-nucleotide ampli-
Wed polymorphism (SNAP) for the CDI25 locus, and two SSRs and cleaved ampliWed polymorphic sequence (CAPS) for CT211 were developed using sequences of the positive BAC clones. These markers will be valuable for rapid selection of genotypes and map-based cloning for resistance genes against P. capsici.
H.-J. Kim and S.-H. Nahm contributed equally to this work.
Communicated by I. Paran.
H.-J. Kim S.-H. Nahm H.-R. Lee G.-B. Yoon B.-C. Kang D. Choi J. H. Ahn J.-H....