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Eur Food Res Technol (2013) 236:873882 DOI 10.1007/s00217-013-1950-9
ORIGINAL PAPER
Biochemical and thermo-mechanical analysis of collagenfrom the skin of Asian Sea bass (Lates calcarifer) and Australasian Snapper (Pagrus auratus), an alternative for mammalian collagen
Sushil Anand Sandip Kamath Lillian Chuang
Stefan Kasapis Andreas L. Lopata
Received: 6 September 2012 / Revised: 16 January 2013 / Accepted: 21 February 2013 / Published online: 10 March 2013 Springer-Verlag Berlin Heidelberg 2013
Abstract Australasia has a large sh industry, and sh skin by-products from the processing industry could be used for the commercial production of sh collagen. The aim of this study was to characterize collagen extracted from the Asian sea bass (Australian barramundi) (Lates calcarifer) and snapper (Pagrus auratus) skin as an alternative to mammalian-derived collagen in gelatin products. The acid-soluble fractions of collagen from Asian sea bass and snapper skin were extracted and yielded about 8 and 7.5 % collagen (on a dry weight basis), respectively. The electro-phoretic and chromatography patterns indicated that both collagens comprise of a1, a2, a3, and b chains, corresponding to the properties of calf skin collagen type I. Amino acid analysis and peptide mapping of digested collagen suggested differences in their amino acid sequences and collagen primary structure. Fourier transform infrared spectroscopy demonstrated that the helical structure of collagen was completely maintained in Asian sea bass and partially in snapper. Transition temperatures for the completion of the melting process in the two collagen networks were conrmed with differential scanning calorimetry and dynamic oscillatory rheology to be about 29 C. Zeta potential analysis identied the isoelectric points (pI values) of collagen from Asian sea bass and snapper skin at pH 6.90 and 7.75, respectively. Thus, Asian sea bass and snapper skin could be an important alternative source of collagen to replace mammalian collagen for industrial applications.
Keywords Collagen Asian sea bass Barramundi
Snapper Thermo-mechanical Skin
Introduction
Collagen is the structural protein found in most vertebrate animals, constituting 30 % of the total animal protein. There are at least 27 different types of collagens which have been identied [1]. Collagen type I has been found to be present in all vertebrate connective tissues including ten-dons, bones, and skins [2]. Collagen has a wide range of applications in cosmetics, food industry, and pharmaceutical industry....