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Abstract
A PATIENT with Philadelphia chromosome-positive chronic myelocytic leukemia in a phase of accelerated growth received a bone marrow transplant from her HLA-identical brother, whose cells were not reactive in the mixed leukocyte culture. Methods Cytogenetics Chromosomes were prepared from 24-hour unstimulated bone marrow cultures with the synchronization technique described by Yunis.1 Determinations of Colony-Stimulating Activity Colony-stimulating activity was measured as described elsewhere by Geissler et al.2 In brief, after being depleted of monocytes and T lymphocytes, low-density bone marrow cells obtained from normal donors were resuspended in McCoy's medium containing 2 percent fetal-calf serum and 0.3 percent agar (Agar-Agar, Merck, Darmstadt, Federal Republic of Germany), plated into wells, and over-layered with 0.25 ml of medium containing various concentrations of serum samples from the patient or a control. Analyses of Surface-Antigen Patterns The expression of different surface antigens of bone marrow cells was measured by direct immunofluorescence and flow cytometry on a fluorescence-activated cell sorter (Becton Dickinson, Mountain View, Calif.) or by immunohistologic examination of cryostat sections of bone marrow. Results of Flow-Cytometric Analysis and Immunohistologic Examination of Donor and Patient Bone Marrow before and after Bone Marrow Transplantation (BMT).* [Image Omitted: See PDF] From the Department of Internal Medicine (D.G., J.T.), the Division of Clinical Immunobiology (D.W.N., G.G., B.M., J.A.T., C.H.), and the Department of Medical Biology and Genetics (E.G.), University of Innsbruck, Innsbruck, Austria; and the Fred Hutchinson Cancer Research Center and Division of Oncology, University of Washington School of Medicine, Seattle (F.R.A.).