Burkholderia SP. Strain TNAU-1 for Biological Control of Root Rot in Mung Bean (Vigna Radiata L.) Caused by Macrophomina Phaseolina

Abstract

[Burkholderia] SP. Strain TNAU-1 for Biological Control of Root Rot in Mung Bean ([Vigna Radiata] L.) Caused by [Macrophomina Phaseolina]

The potential of [Burkholderia] sp. strain TNAU-1 for the management of mung bean ([Vigna radiata] L.) root rot caused by [Macrophomina phaseolina] was evaluated under greenhouse conditions. [Burkholderia] sp. strain TNAU-1 inhibited the mycelial growth of [M. phaseolina in vitro] and produced an inhibition zone of 18.8 mm. Mung bean seeds when treated with the bacterial suspension, showed significant increase in root length, shoot length and seedling vigour. A talc-based powder formulation of [Burkholderia] sp. strain TNAU-1 was developed and evaluated for its efficacy in the management of mung bean root rot under greenhouse conditions. Seed treatment or soil application of the powder formulation of [Burkholderia] sp. strain TNAU-1 significantly reduced the incidence of root rot and increased the germination percentage and plant height. Seed treatment with the powder formulation of [Burkholderia] sp. strain TNAU-1 alone was effective in controlling root rot disease; but the combined seed treatment and soil application of [Burkholderia] sp. strain TNAU-1, increased the efficacy. Seed treatment and soil application with [Burkholderia] sp. reduced the root rot incidence from 52.6 per cent (with non-bacterized seeds) to 16.7 per cent. Control of root rot with the application of [Burkholderia] sp. by seed treatment and soil application was not statistically different from that obtained with seed treatment with carbendazim. The endophytic movement of [Burkholderia] sp. in the stem, roots and leaves of mung bean was confirmed through PCR using [Burkholderia] sp. specific primers which resulted in the amplification of a 417 bp product.