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Fish Physiol Biochem (2015) 41:177191 DOI 10.1007/s10695-014-0015-8
cDNA structure and the effect of fasting on myostatin expression in walking catsh (Clarias macrocephalus, Gnther 1864)
Poonmanee Kanjanaworakul Prapansak Srisapoome
Orathai Sawatdichaikul Supawadee Poompuang
Received: 21 July 2014 / Accepted: 21 November 2014 / Published online: 29 November 2014 Springer Science+Business Media Dordrecht 2014
Abstract We cloned and sequenced the myostatin (MSTN) gene of walking catsh and characterized its expression under different conditions. The full cDNA sequence of MSTN was 1,784 bp, containing an open reading frame of 1,191 bp, which encoded 396 amino acids. The deduced MSTN sequence contained functional sites similar to other members of TGF-b superfamily, including the proteolytic processing site and nine conserved cysteines in the C-terminal. Walking catsh MSTN mRNA was strongly expressed in skeletal muscle and brain tissues, consistent with
the expression proles of MSTN-1 isoform in other teleosts. Temporal expression analysis revealed that the MSTN was expressed at the highest levels in 1-week-old larvae and adults, but was lowest in early juveniles. A fastingre-feeding experiment was used to evaluate the effects of starvation on growth and MSTN expression in juvenile walking catsh for 28 days. MSTN transcript levels increased signicantly (threefold) after 7 days of fasting (P \ 0.05)
compared with the fed control. Subsequently, MSTN expression levels decreased 1.6-fold when fasting was extended to 14 days. Although re-feeding decreased the MSTN expression relative to the levels of the fed control, the period was not long enough for growth recovery of the juveniles. Our results supported a role of MSTN as a negative regulator of muscle growth and, possibly, a role in energy conservation in sh.
Keywords Clarias macrocephalus Myostatin
cDNA cloning Muscle growth Gene expression
Introduction
Fish display a unique pattern of muscle growth and development as, unlike mammals and birds, in sh the recruitment of new myobers continues throughout much of the life cycle (reviewed by Johnston et al. 2011). As in other vertebrates, muscle growth and development in sh are controlled by numerous
Electronic supplementary material The online version of this article (doi:http://dx.doi.org/10.1007/s10695-014-0015-8
Web End =10.1007/s10695-014-0015-8 ) contains supplementary material, which is available to authorized users.
P. KanjanaworakulCenter for Agricultural Biotechnology, Kasetsart University, Nakhon Pathom 73140, Thailand
P. KanjanaworakulCenter of Excellence on Agricultural Biotechnology (AG-BIO/PERDO-CHE), Bangkok 10900,...