Key words: Varicocele, Celecoxib, Silymarin, Inflammation, Oxidative Stress
P-173
Introduction: Varicocele (VCL) results in considerable disorders in male reproductive potential. Varicocele exerts its impact via enhancing inflammation and down- regulating testicular endocrine and antioxidant statuses. Celecoxib (CCB) is a non-steroidal anti-inflammatory drug (NSAID), which is used as cyclooxygenase-2 (COX-2) inhibitor. Silymarin (SMN) is a flavonoid complex, which is known for its antioxidant potential. Present study was designed for evaluating the protective effect of CCB and SMN on VCL-induced damages in testicles and sperm cells.
Materials and Methods: VCL was induced in 24 mature male rats and then animals were randomly divided into four groups including; non-treated VCL- induced, CCB-treated (10mg/kg, orally), SMN-treated (100mg/kg, orally), SMN+CCB-treated groups. Following 60 days after VCL induction, spermatogenesis ratio evaluated by assessing tubular differentiation (TDI), repopulation (RI) indexes. Leydig cells steroid foci were evaluated by using epifluorescent staining. Immune cels infiltration, tissue total antioxidant capacity (TAC) and malondialdehyde (MDA) content as well as sperm count, motility, abnormality and DNA damage were analyzed.
Results: CCB and SMN co-administration elevated VCL-reduced TDI, RI and SPI and reduced immune cells infiltration. The Leydig cells steroidogenic foci increased in CCB+SMN, SMN and CCB-treated groups, respectively. SMN+CCB significantly (p<0.05) up- regulated VCL-reduced TAC and reduced tissue MDA. All treated animals exhibited remarkably (p<0.05) higher sperm count, sperm motility, viability and reduced sperm abnormality and DNA damage. However it was more pronounced in SMN+CCB-treated group.
Conclusion: Our data suggest that, SMN inhibited the VCL-induced damages via up-regulating antioxidant status and CCB limited the disorders by attenuating VCL-induced inflammation.
Mazhari S1, Razi M2, Malekinejad H3, Sadrkhanlou R2.
1. Faculty of Veterinary Medicine, Urmia Univercity, Urmia, Iran.
2. Department of Comparative Histology and Embryology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
3. Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Email: [email protected]
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Copyright Yazd Shahid Sadoughi University of Medical Sciences, Research and Clinical Center for Infertility Jun 2014