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Int J Hematol (2009) 89:497507 DOI 10.1007/s12185-009-0303-8
ORIGINAL ARTICLE
Clinical signicance of co-expression of CD21 and LFA-1 in B-cell lymphoma
Kazushi Tanimoto Yoshihiro Yakushijin Hiroshi Fujiwara Masaki Otsuka Koichi Ohshima Atsuro Sugita Akira Sakai Takaaki Hato Hitoshi Hasegawa Masaki Yasukawa
Received: 13 November 2008 / Revised: 8 March 2009 / Accepted: 12 March 2009 / Published online: 11 April 2009 The Japanese Society of Hematology 2009
Abstract We previously reported that the prognosis of CD21-positive diffuse large B-cell lymphoma (DLBCL) is signicantly favorable to that of CD21-negative DLBCL (Otsuka et al. in Br J Haematol 127:416424, 2004). In this study, we attempted to clarify the biological signicance of CD21 expression in B-cell lymphoma (BCL) by performing in vitro experiments using CD21 transfection into a CD21-negative lymphoma cell line and analyzing clinical
data from lymphoma samples. Established clones of CD21 transfectants showed homotypic aggregation in suspension culture. Analysis of integrin expression revealed that LFA-1 appeared to be expressed on CD21 transfectants, and the cell aggregation was abrogated by anti-LFA-1 antibody. The CD21 transfectants could adhere to plastic plates coated with ICAM-1. Moreover, ow cytometry and/ or immunohistochemical analyses of clinical BCL samples (n = 29) revealed positive for CD21 in all cases; LFA-1 was also expressed without exception. All BCL cells isolated from cavity uids (n = 10) failed to express both CD21 and LFA-1. These data suggest that CD21 is tightly related to LFA-1 expression in BCL and the absence of CD21/LFA-1 expression is associated with pleural/perito-neal uid involvement by BCL, a potential indicator of disease progression of BCL.
Keywords CD21 LFA-1 B-cell lymphoma
1 Introduction
Since non-Hodgkin lymphoma (NHL) includes a heterogeneous set of lymphoid malignancies, precise classication is essential for predicting the prognosis and determining the appropriate treatment strategy. Although recent studies using gene expression prole-based classication have successfully discriminated diffuse large B-cell lymphoma (DLBCL) into two subgroups reecting the origin of the lymphoma cells and the disease prognosis [14], this method is complex and difcult to apply in routine clinical practice. Conversely, immunohistochemistry-based classication of NHL is readily applicable in clinical practice and has been widely used. Various surface markers are used for classication of NHL; however, associations between immunological
K. Tanimoto H. Fujiwara M. Otsuka H. Hasegawa
M. Yasukawa (&)
Department...