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Abstract Sulfuric acid-phenol and sulfuric acid-anthrone methods were used to detect polysaccharide content in shoots of Aralia elata ( Miq. ) Seem. , and the conversion factor to glucose was measured with refinedmed polysaccharides. Comprehensive valuation was carried out by linear relationship, precision, reproducibility, stability and recovery rate. The results showed that the linear relationship between glucose concentration and absorbance was good when glucose concentration was 0 -40 µg/ml, and the average recovery rate was equal to or higher than 97. 00% with good reproducibility (RSD < 1. 60% , n = 5). It revealed that the two methods were accurate and reliable, and suitable for the determination of polysaccharide content in the shoots of A. elata. Polysaccharide content detected by sulfuric acid-phenol and sulfuric acid-anthrone methods was 19. 31% and 20.40% respectively.
Key woros Shoots of Arali<l elata ( Miq. ) Seem. ; Polysaccharides; Sulfuric acid-phenol method; Sulfuric acid-anthrone method
Aralia elata ( Miq. ) Seem. is a perennial deciduous tree of Aralia Linn. of the Araliaceae. It is commonly known as northeast Chinese toon 1 1. As a traditional medicinal and food plant with high medicinal and edible value , it is can be used to tonify Qi , tonify spleen , dispel wind and eliminate dampness , promote blood circulation and stop pain. Modem studies have shown that A. elata is mainly used to cure neurasthenia, constipation, stomach cramps, diabetes , rheumatoid arthritis , hepatitis and other diseases 1 2 1.However, most studies on A. elata are focused on the content of chemical constituents such as total saponins and total flavonoids and their pharmacological activities in its root bark and stem bark, while there are few studies on the determination of polysaccharide content in the shoots of A. elata[ 'l. Polysaccharides in A. elata are a type of neutral heteropolysaccharides with the molecular weight of about 8 000 , and can be mainly hydrolyzed into monosaccharides such as arabinose, glucose and galactose 4 1 .The pharmacological activities of polysaccharides in suppressing tumors , immune regulation, lowering the concentration of glucose in the blood, reducing blood fat , destroying or inhibiting the growth and reproduction of viruses, and resisting radiation have attracted the attention of the medical community at home and abroad....