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Abstract
A simple, rapid, gradient reversed phase Ultra Performance Liquid Chromatographic method with ultraviolet detection (UPLC-UV) was developed for analysis of Albuterol sulfate and its impurities in Albuterol sulfate inhalation solution pharmaceutical formulation. Chromatography was carried out on a Waters Acquity BEH C18 (1.7μm, 100mm.x 2.1mm) column using 0.02 M Potassium Dihydrogen Orthophosphate as mobile phase A and Acetonitrile as mobile phase B. at a flow rate of 0.150mL/min and a 210 nm detection. Results from table 3 and 4 showed that Ultra Performance Liquid Chromatography exhibited a rapid and separation efficiency superior to that of existing conventional HPLC method.
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