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Eur J Nutr (2015) 54:149156 DOI 10.1007/s00394-014-0696-x
ORIGINAL CONTRIBUTION
Consumption of a dark roast coffee decreases the levelof spontaneous DNA strand breaks: a randomized controlled trial
T. Bakuradze R. Lang T. Hofmann
G. Eisenbrand D. Schipp J. Galan
E. Richling
Received: 18 October 2013 / Accepted: 28 March 2014 / Published online: 17 April 2014 Springer-Verlag Berlin Heidelberg 2014
AbstractPurpose Coffee consumption has been reported to decrease oxidative damage in peripheral white blood cells (WBC). However, effects on the level of spontaneous DNA strand breaks, a well established marker of health risk, have not been specically reported yet. We analyzed the impact of consuming a dark roast coffee blend on the level of spontaneous DNA strand breaks.
Methods Healthy men (n = 84) were randomized to consume daily for 4 weeks either 750 ml of fresh coffee brew or 750 ml of water, subsequent to a run in washout phase of 4 weeks. The study coffee was a blend providing high amounts of both caffeoylquinic acids (10.18 0.33 mg/g) and the roast product N-methylpyridinium(1.10 0.05 mg/g). Before and after the coffee/water consumption phase, spontaneous strand breaks were determined by comet assay.
Results At baseline, both groups exhibited a similar level of spontaneous DNA strand breaks. In the intervention phase, spontaneous DNA strand breaks slightly increased in the control (water only) group whereas they signicantly
decreased in the coffee group, leading to a 27 % difference within both arms (p = 0.0002). Food frequency questionnaires indicated no differences in the overall diet between groups, and mean body weight during the intervention phases remained stable. The consumption of the study coffee substantially lowered the level of spontaneous DNA strand breaks in WBC.
Conclusion We conclude that regular coffee consumption contributes to DNA integrity.
Keywords Antioxidants Coffee Comet assay
Intervention study DNA strand breaks
AbbreviationsBMI Body mass indexTI Tail intensityBS Blood samplingNMP N-MethylpyridiniumNQO1 NAD(P)H:quinine oxidoreductase 1 c-GCL c-Glutamylcysteine ligase
Nrf2 NF-E2 p45 subunit-related factor 2
Introduction
Coffee is one of the most popular beverages worldwide. Several in vivo studies suggest that coffee consumption is associated with prevention or delay of degenerative diseases including diabetes mellitus type two, Parkinson, cardiovascular disease or cancer [1]. The pathogenesis of these diseases is associated with increased production of reactive oxygen species and...