A critical review on some closely related species of Tetranychus sensu stricto (Acari: Tetranychidae) in the public DNA sequences databases

Exp Appl Acarol (2011) 55:123 DOI 10.1007/s10493-011-9453-5

Renata S. de Mendona Denise Navia Ivone R. Diniz

Philippe Auger Maria Navajas

Received: 3 October 2010 / Accepted: 21 March 2011 / Published online: 6 April 2011 Springer Science+Business Media B.V. 2011

Abstract Taxonomic misidentication of the specimens used to obtain DNA sequences is a growing problem reported for different groups of organisms, which threatens the utility of the deposited sequences in public DNA databases. This paper provides new evidence of misidentications in molecular DNA public databases in phytophagous mites of the Tetranychidae family belonging to the group Tetranychus (Tetranychus). Several species in this group are of economic and quarantine importance in agriculture and among them Tetranychus urticae, a highly polyphagous mite causing outbreaks in many crops worldwide, is certainly the most studied. We analyzed and evaluated the identity of 105 Gen-Bank accessions of ITS2 rDNA and 138 COI mtDNA sequences which were deposited asT. urticae and those of 14 other taxa morphologically closely related to Tetranychus sensu stricto. In addition, ITS2 and COI sequences of 18 T. urticae samples collected for this study and identied by morphological criteria, were generated and included in the analyzed dataset. Among the deposited sequences in the GenBank database, numerous cases of

Electronic supplementary material The online version of this article (doi:http://dx.doi.org/10.1007/s10493-011-9453-5

Web End =10.1007/s10493-011-9453-5 ) contains supplementary material, which is available to authorized users.

R. S. de Mendona (&) D. Navia

Plant Quarantine, Embrapa Recusrsos Genticos e Biotecnologia, Parque Estao Biolgica, Norte Final W5, Caixa Postal 02372, Braslia, DF 70770-917, Brazile-mail: [email protected]

D. Naviae-mail: [email protected]

I. R. Diniz

Departamento de Zoologia, UNB, Instituto de Cincia Biolgicas, Universidade de Braslia, Campus Universitrio Darcy Ribeiro, ICC Sul, Sala AT-116, Asa Norte, Braslia, DF 70910-900, Brazil e-mail: [email protected]

P. Auger M. Navajas

Intitut National de la Recherche Agronomique, UMR CBGP (INRA/IRD/Cirad/Montpellier SupAgro), Campus International de Baillarguet, CS 30016, 34988 Montferrier-sur-Lez Cedex, Francee-mail: [email protected]

M. Navajase-mail: [email protected]

A critical review on some closely related speciesof Tetranychus sensu stricto (Acari: Tetranychidae) in the public DNA sequences databases

123

2 Exp Appl Acarol (2011) 55:123

apparently mistaken identities were identied in the group Tetranychus s. str., especially between T. urticae, T. cinnabarinus, T. kanzawai and T. truncatus. Unreliable sequences (misidentied or dubious) were estimated at nearly...