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Appl Biochem Biotechnol (2011) 163:433443
DOI 10.1007/s12010-010-9051-8
Selva Onder & Mithat Celebi & Melda Altikatoglu & Arzu Hatipoglu & Huriye Kuzu
Received: 27 May 2010 /Accepted: 19 July 2010 /
Published online: 12 August 2010# Springer Science+Business Media, LLC 2010
Abstract This study evaluates the potential of the enzyme horseradish peroxidase in the decolorization of one common industrial azo dye, naphthol blue black. Studies are carried out to understand the process parameters such as pH, temperature and reaction time. The enzymatic decolorization of the dye was examined by UV-Vis spectrophotometer and LC-MS measurements. Temperature and pH conditions were optimized for obtaining high azo-dye decolorization. Azo-dye removal at a pH range 4-6 was found to be the highest for all temperatures. After 5 minutes of treatment, the color removal of dye was ca. 80-90%. The LC-MS and spectrophotometric analyses indicated that the decolorization of the azo dye with enzyme was due to the reduction of the azo bonds. This study verifies the viability of the use of the horseradish peroxidase in the decolorization of naphthol blue black.
Keywords Dye decolorization . Horseradish peroxidase . Naphthol blue black . Spectrophotometer . LS-MS
Introduction
The treatment of industrial effluents is a challenging topic in environmental science, as control of water pollution has become of increasing importance in recent years [1]. Water pollution due to discharge of coloured effluents from textile dye manufacturing and textile dyeing mills is one of the major environmental concerns in todays world. Strong colour
S. Onder : M. Altikatoglu (*) : A. Hatipoglu
Faculty of Arts and Sciences, Department of Chemistry, Yildiz Technical University, Davutpasa Campus 34210 Esenler, Istanbul, Turkeye-mail: [email protected]
M. Celebi
Faculty of Engineering, Department of Polymer Engineering, Yalova University, 77100 Yalova, Turkey
H. Kuzu
Faculty of Chemical and Metallurgical Engineering, Department of Bioengineering, Yildiz Technical University, Davutpasa Campus 34210 Esenler, Istanbul, Turkey
Decolorization of Naphthol Blue Black using the Horseradish Peroxidase
434 Appl Biochem Biotechnol (2011) 163:433443
imparted by dyes to the receiving aquatic ecosystems poses aesthetic problems as well as serious ecological problems, such as carcinogenicity and inhibition of benthic photo-synthesis. Therefore, a number of techniques aimed at preferential removal of dyes from wastewater have been developed [25]. Azo dyes have been widely used as colourants in a variety of...