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ABSTRACT:
A simple, precise, accurate and reproducible spectrophotometric method has been developed for simultaneous estimation of Lornoxicam (LXM) and Eperisone (EPE) by employing first order derivative zero crossing method in methanol. The first order derivative absorption at 264.5 nm (zero cross point of LXM) was used for quantification of EPE and 254 nm (zero cross point of EPE) for quantification of LXM. The linearity was established over the concentration range of 2-24 µ g/ml with correlation coefficient 0.9987 and 0.997, respectively. The mean % recoveries were found to be in the range of 99.69 and 99.13 for LXM and EPE respectively. The developed method has been validated as per ICH guidelines and successfully applied to the estimation of LXM and EPE in their Synthetic Mixture.
KEYWORDS:
INTRODUCTION:
Lornoxicam (LXM) is chemically (3E)-6-chloro-3[hydroxyl (pyridin-2-ylamino) methylene]-2 methyl-2,3dihydro - 4H- thieno [2,3-e] [1,2] thiazin-4-one 1, 1dioxide.[1] Lornoxicam is an NSAID of the oxicam class with analgesic, anti-inflammatory and antipyretic properties. It inhibits prostaglandin synthesis by inhibiting both cyclo-oxygenase enzyme (COX-1 and COX-2). EPE is chemically (2RS)-1-(4-ethylphenyl)-2methyl-3-(1-piperidyl) propane-1-one.[2] EPE is an antispasmodic drug. It acts by relaxing both skeletal muscles and vascular smooth muscles, and demonstrates a variety of effects such as reduction of myotonia, improvement of circulation, and suppression of the pain reflex. The review of literature revealed that various involving spectrophotometry have been reported for LXM in single form and in combination with other drugs.
Lornoxicam is estimated by UV and HPLC method.[3-17] According to literature review, Eperisone is estimated by UV, HPLC, LC-EI-MS methods.[18] There is no any method reported for Simultaneous estimation of Lornoxicam and Eperisone in a combination by UV and HPLC, but individually available for each drug and in combination with other drug. The present work describes the development of a simple, precise, accurate and reproducible spectrophotometric method for the simultaneous estimation of LXM and EPE in synthetic mixture. The developed method was validated in accordance with ICH Guidelines and successfully employed for the assay of LXM and EPE in synthetic mixture.
MATERIALS AND METHODS:
Reagents and Chemicals:
Analytically pure LXM and EPE were kindly provided by Cirex Pharmaceuticals Limited. Analytical grade methanol was purchased from Merck Pvt. Ltd., India.
Instrument and Apparatus:
Shimadzu-1800 UV-Visible Spectrophotometer was used for spectral measurements...