Different HPV subtypes E6/E7 genes expression analysis in cervical dysplasia

Headnote

Keywords: HPV, HPV DNA, E6/E7 mRNA, cervical dysplasia

[PP-005]

Objective: Determining the E6 and E7 carcinogenic proteins caused by various HPV sub-types, and investigating their effects on cervical dysplasia.

Material and Methods: Seventy seven patients who were 21 years old or over, and whose smear results were reported as ASCUS, ASC-H, LSIL, HSIL and AGC were included in the study. In order to determine the HPV DNA and the HPV types, the HPV sign Q24 Complete Kit, the RotorGene and PyroMark Q24 (Qiagen, Germany) systems were used. Then, by using the NucliSENS EasyQ Genetic Analyzer device (Biomerieux, France), and utilizing the NASBA Method, the existence of E6 and E7 gene expression in HPV DNA positive samples was investigated with total fragment analysis method. The SPSS 22.0 Program was used in statistical analyses.

Results: When the HPV DNA was investigated in 77 cases (100%) who had abnormal sitology it was observed that in 22 (28,6%) cases the HPV DNA was positive, and in 55 (71,4%) cases the HPV DNA was negative. In 22 HPV DNA positive cases, the HPV E6/E7 mRNA was investigated; 8 cases were ASCUS and 3 (37,5%) cases had positive E6/E7 mRNA, 4 cases were ASC-H and 3 (75%) cases had positive E6/ E7 mRNA, 8 cases were LGSIL and 4 (50%) had positive E6/E7 mRNA, 2 cases were HGSIL and 1 (50%) had positive E6/E7 mRNA.

Discussion: In our study, it has been determined that there is a direct relation between the increasing positivity rate of the HPV E6/E7 mRNA gen expression and the frequency of cervical dysplasia. This rate was determined as being statistically significant.