Digging deep and wide into single cells

Abstract

The myriad physical changes that can be observed in an individual cell under different environmental conditions represent a wealth of information for scientists to mine. This information can be captured by imaging cells under many experimental perturbations at a resolution sufficient to observe subcellular details. For such high-content screening, scientists must design their experiments to maximize their instruments functionality. Researchers also need to plan data analysis well before a data mountain stacks up in front of them.