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Extremophiles (2009) 13:623632 DOI 10.1007/s00792-009-0245-3
ORIGINAL PAPER
Diversity of Actinobacterial community in saline sediments from Yunnan and Xinjiang, China
Jinyuan Wu Tongwei Guan Hongchen Jiang Xiaoyang Zhi Shukun Tang Hailiang Dong Lili Zhang Wenjun Li
Received: 30 November 2008 / Accepted: 31 March 2009 / Published online: 7 June 2009 Springer 2009
Abstract The diversity and community structures of actinobacteria in saline sediments collected from Yunnan and Xinjiang Provinces, China, were investigated with cultivation and 16S rRNA gene analysis. A total of 163 actinobacterial isolates were obtained, and they were afliated with the order Actinomycetales (distributed into ve suborders: Streptosporangineae, Micrococcineae, Streptomycineae, Pseudonocardineae, and Glycomycineae). A total of 748 actinobacterial 16S rRNA gene clones were examined, and they could be classied into Actinomycetales,
Acidimicrobiales, and unclassied actinobacteria. The Actinomycetales sequences were distributed into nine suborders: Streptosporangineae, Glycomycineae, Micro-monosporineae, Pseudonocardineae, Corynebacterineae, Frankineae, Propionibacterineae, Streptomycineae, and Micrococcineae. The unclassied actinobacteria contained three new clusters at the level of subclass or order. Our 16S rRNA gene phylogenetic data indicated that actinobacterial communities were very diverse in the investigated saline sediments (salinity 0.411.6%) and some actinobacterial members may be halotolerant or halophilic. The actino-bacterial community structures in the saline sediments were different from those in marine and freshwater environments. Our data have implications for a better understanding of the distribution of Actinobacteria in saline environments.
Keywords Saline sediments Salinity Actinobacteria
Diversity
Introduction
The Actinobacteria phylum, one of the main phyla within the Bacteria, contains four classied subclasses: Rubrobacteridae, Coriobacteridae, Acidimicrobidae, and Actinobacteridae, and some unclassied Actinobacteria (Boone et al. 2001; Hugenholtz and Stackebrandt 2004; Gao and Gupta 2005). Due to their biotechnological applications, Actinobacteria are drawing increasing attention from microbiologists (Ward and Bora 2006). Actino-bacteria are ubiquitous in soils (Cho et al. 2006; Gremion et al. 2003; Piao et al. 2008; and references therein), marine environments (Bull et al. 2005; Goodfellow and Haynes 1984; Maldonado et al. 2005; Stach and Bull 2005; Stach et al. 2003a; Ward and Bora 2006), and freshwater
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J. Wu X. Zhi S. Tang W. Li (&)
The Key Laboratory for Microbial Resources of the Ministry of Education,...