Content area
Abstract
We report that low percentages of dimethylsulfoxide (DMSO) in liquid chromatography solvents lead to a strong enhancement of electrospray ionization of peptides, improving the sensitivity of protein identification in bottom-up proteomics by up to tenfold. The method can be easily implemented on any LC-MS/MS system without modification to hardware or software and at no additional cost. [PUBLICATION ABSTRACT]
Details
Title
DMSO enhances electrospray response, boosting sensitivity of proteomic experiments
Author
Hahne, Hannes; Pachl, Fiona; Ruprecht, Benjamin; Maier, Stefan K; Klaeger, Susan; Helm, Dominic; Médard, Guillaume; Wilm, Matthias; Lemeer, Simone; Kuster, Bernhard
Pages
989-91
Publication year
2013
Publication date
Oct 2013
ISSN
15487091
e-ISSN
15487105
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
1458601363
Copyright
Copyright Nature Publishing Group Oct 2013