Dynamics of Sylvatic Chagas Disease Vectors in Coastal Ecuador Is Driven by Changes in Land Cover: e2960

Abstract

Background

Chagas disease is a serious public health problem in Latin America where about ten million individuals show Trypanosoma cruzi infection. Despite significant success in controlling domiciliated triatomines, sylvatic populations frequently infest houses after insecticide treatment which hampers long term control prospects in vast geographical areas where vectorial transmission is endemic. As a key issue, the spatio-temporal dynamics of sylvatic populations is likely influenced by landscape yet evidence showing this effect is rare. The aim of this work is to examine the role of land cover changes in sylvatic triatomine ecology, based on an exhaustive field survey of pathogens, vectors, hosts, and microhabitat characteristics' dynamics.

Methodology and Principal Findings

The study was performed in agricultural landscapes of coastal Ecuador as a study model. Over one year, a spatially-randomized sampling design (490 collection points) allowed quantifying triatomine densities in natural, cultivated and domestic habitats. We also assessed infection of the bugs with trypanosomes, documented their microhabitats and potential hosts, and recorded changes in landscape characteristics. In total we collected 886 individuals, mainly represented by nymphal stages of one triatomine species Rhodnius ecuadoriensis. As main results, we found that 1) sylvatic triatomines had very high T. cruzi infection rates (71%) and 2) densities of T. cruzi-infected sylvatic triatomines varied predictably over time due to changes in land cover and occurrence of associated rodent hosts.

Conclusion

We propose a framework for identifying the factors affecting the yearly distribution of sylvatic T. cruzi vectors. Beyond providing key basic information for the control of human habitat colonization by sylvatic vector populations, our framework highlights the importance of both environmental and sociological factors in shaping the spatio-temporal population dynamics of triatomines. A better understanding of the dynamics of such socio-ecological systems is a crucial, yet poorly considered, issue for the long-term control of Chagas disease.

Abstract

Background

Chagas disease is a serious public health problem in Latin America where about ten million individuals show Trypanosoma cruzi infection. Despite significant success in controlling domiciliated triatomines, sylvatic populations frequently infest houses after insecticide treatment which hampers long term control prospects in vast geographical areas where vectorial transmission is endemic. As a key issue, the spatio-temporal dynamics of sylvatic populations is likely influenced by landscape yet evidence showing this effect is rare. The aim of this work is to examine the role of land cover changes in sylvatic triatomine ecology, based on an exhaustive field survey of pathogens, vectors, hosts, and microhabitat characteristics' dynamics.

Methodology and Principal Findings

The study was performed in agricultural landscapes of coastal Ecuador as a study model. Over one year, a spatially-randomized sampling design (490 collection points) allowed quantifying triatomine densities in natural, cultivated and domestic habitats. We also assessed infection of the bugs with trypanosomes, documented their microhabitats and potential hosts, and recorded changes in landscape characteristics. In total we collected 886 individuals, mainly represented by nymphal stages of one triatomine species Rhodnius ecuadoriensis. As main results, we found that 1) sylvatic triatomines had very high T. cruzi infection rates (71%) and 2) densities of T. cruzi-infected sylvatic triatomines varied predictably over time due to changes in land cover and occurrence of associated rodent hosts.

Conclusion

We propose a framework for identifying the factors affecting the yearly distribution of sylvatic T. cruzi vectors. Beyond providing key basic information for the control of human habitat colonization by sylvatic vector populations, our framework highlights the importance of both environmental and sociological factors in shaping the spatio-temporal population dynamics of triatomines. A better understanding of the dynamics of such socio-ecological systems is a crucial, yet poorly considered, issue for the long-term control of Chagas disease.

Author Summary

Globally, more than 10 million people are infected with Trypanosoma cruzi. The emergence and perpetuation of Chagas disease in some endemic countries, such as Ecuador, depends largely on sylvatic populations of T. cruzi-infected vectors that frequently infest houses after insecticide treatment thereby hampering long-term control prospects in vast geographical areas. Our study describes, for the first time in an agricultural landscape, how the temporal dynamics of sylvatic vector, host, and pathogen populations interact spatially in a farming community of coastal Ecuador. In particular, we found that land cover changes due to both farming activities and vegetation phenology affect rodent host distribution and consequently the relative abundance of vectors involved in the transmission cycle of T. cruzi.

Citation: Grijalva MJ, Terán D, Dangles O (2014) Dynamics of Sylvatic Chagas Disease Vectors in Coastal Ecuador Is Driven by Changes in Land Cover. PLoS Negl Trop Dis 8(6): e2960. doi:10.1371/journal.pntd.0002960

Editor: Ricardo E. Gürtler, Universidad de Buenos Aires, Argentina

Received: November 21, 2013; Accepted: May 8, 2014; Published: June 26, 2014

Copyright: © 2014 Grijalva et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Funding: This work was supported by Pontifical Catholic University of Ecuador, Plan International Ecuador, the Ecuadorian Public Health Ministry, Ohio University Heritage College of Osteopathic Medicine, Children's Heartlink and the European Commission Framework Programme Project "Comparative epidemiology of genetic lineages of Trypanosoma cruzi" ChagasEpiNet, Contract No. 223034. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing interests: The authors have declared that no competing interests exist.

Introduction

Chagas disease is a vector-borne disease caused by a flagellated protozoan parasite (Trypanosoma cruzi) that affects approximately 10 million people in Latin America and the Caribbean region [1], with an increasing number of cases reported in non-endemic countries [2]. The vectors are blood-sucking reduviid bugs of the subfamily Triatominae, of which 70 of the over 140 Triatominae species described [3], [4] have been found to be naturally infected with T. cruzi [5]. Originally restricted to sylvatic habitats, Chagas disease began to present a risk to human health as its vectors acquired the ability to colonize and sustain populations in human dwellings, resulting in the domiciliary transmission of T. cruzi [6]. In addition to domiciliated species, there is increasing evidence from several countries (e.g., Bolivia [7], Ecuador [8], [9], Brazil [4], Argentina [10]) that some sylvatic individuals frequently colonize human habitats and may play a key role in the transmission cycle of T. cruzi. Moreover, recent studies conducted in Ecuador indicate that house infestation, likely originating from sylvatic areas, limits the effectiveness of insecticide-based control interventions [11]. To improve our understanding of the colonization and domiciliation processes, monitoring and understanding the dynamics of sylvatic species/individuals that may colonize anthropogenic habitats has therefore become a cornerstone issue in Chagas disease research field [3].

The temporal and spatial dynamics of vector populations in sylvatic habitats is strongly influenced by the structure of the landscape and its evolution (either under natural or anthropogenic drivers) through time [12]-[14]. For example, in the Pantanal region of Brazil, changes in vegetation cover according to variations in multi-year flooding intensity strongly affect the availability of habitats occupied by rodent hosts, resulting in complex trypanosome transmission cycles [15]. Consequently, land cover modifications (e.g. ranching extension, deforestation) may affect the role that small mammals play in the transmission cycle of trypanosomes [16]. More generally, anthropogenic and natural habitat modifications increase the contact between wildlife and domestic animals, or vectors and hosts, and modify the epidemiological profiles of transmission cycles, with potential consequences on disease emergence or re-emergence patterns [17]. Moreover, the transmissibility pathways of multiple host parasites, as it is the case for T. cruzi, may change according to any factor affecting the population dynamics of their hosts [16]. Monitoring the sylvatic transmission cycles of these trypanosomatids is therefore necessary to prevent re-emergence of Chagas disease.

The objective of this work was to assess whether temporal changes in land cover characteristics may affect the spatial distribution and host association of T. cruzi vectors. The study was performed in agricultural landscapes of coastal Ecuador as a study model. In this country, around 3.8 million people are at risk of acquiring Chagas disease and some 200,000 individuals are currently infected with the disease, making it a serious public health issue [18]. Over one year, we sampled triatomine individuals in natural (forest or shrub), cultivated and domestic habitats; assessed their infection with trypanosomes; and recorded changes in landscape characteristics. As the distribution of sylvatic triatomine species has been found to be related to the presence of rodent hosts (mainly mice, rats and squirrels [8]) themselves influenced by vegetation type and cover, we hypothesized that temporal land cover changes partly related to the crop production cycle (planting, harvesting, absence of crop) may affect the presence of rodent hosts and consequently that of associated triatomines.

Materials and Methods

Ethics Statement

We obtained informed consent from the head of the household following protocols approved by the institutional review boards of Ohio University and Catholic University of Ecuador (PUCE).

Study Site

Our study was conducted in the rural community of El Bejuco located in Portoviejo County, Manabí Province (0°57'37.80"S; 80°14'0.51"W) in northwestern Ecuador. This community was chosen for the high triatomine baseline and post-insecticide spraying household infestation [11] and the presence of a landscape composed of both natural and cultivated areas. This community comprised 98 houses with an approximate number of 450 inhabitants. Most houses are constructed with bamboo walls, concrete bricks and timber with roofs made of corrugated metal sheets or Phytelephas aequatorialis Spruce palm leaves [19]. The peridomestic areas contained dogs, chickens, pigs and cows dwelling among firewood piles and trees [11]. Average monthly temperatures range from 18.7 to 30.8°C with a mean value of 24.8°C. The precipitation regime is characterized by two main seasons: 1) a rainy season (total precipitation = 779 mm) between January and April and 2) a dry season (total precipitations = 26 mm) between July and October (data from the WorldClim data set). The average relative humidity ranges between 90 and 97% over the year [8].

Triatomine Sampling

Our sampling area in the community was defined as a 1000 m×600 m quadrat located along the main road of the village (Figure 1). This quadrat included the different environments found in the community (synanthropic [defined as peridomestic and domestic habitats], cultivated areas [crops], and natural vegetation [forest or shrub]). Sampling was performed on seven visits from June 2009 to June 2010, with intervals of two months between visits. Two visits (February and April) were performed during the rainy season, two (August and October) during the dry season, and three (June and December 2009, and June 2010) during the transition periods. Triatomine sampling in synanthropic environments (12 houses located in the sampling area) was conducted on every visit by a three-person team using standardized protocols during timed manual collections as previously described [20]. If at least one live triatomine was found in a domestic unit (DU), this house was classified as being infested and was sprayed by trained field workers from the Ministry of Health with deltamethrin at a rate of 25 mg a.i per m2 as previously described [11]. Spraying included all structures found in the domicile and peridomicile and were supervised by staff from PUCE. Houses found to be not-infested were not sprayed.

[Figure omitted, see PDF]

Figure 1. Map of the study area indicating the location of the quadrat, digital representation of cultivated areas [crops], and natural vegetation [forest or shrub]) and an overlay of the road and house locations.

Insert indicates the location of the study area within the political map of Ecuador.

Sylvatic populations of triatomines (defined as populations sampled in both cultivated and natural vegetation) were sampled following the randomized sampling design developed by Suarez-Davalos et al. [8]. During each visit the coordinates of 70 points were randomly generated within the quadrat using the Spatstat package of R Software (R Development Core Team 2012). In the case that the distance between two points was <3 m, the whole randomization was rerun (see [8] for more details). For all dates, the minimal distance between each collection point was 10.2 m. These coordinates were then transferred to a GPS receiver (Meridian Platinum, Magellan, San Dimas, United States) for field sampling. At each point, standard manual searches for triatomines were performed for 30 min by three-person teams in different microhabitats (nests, burrows, tree holes, and under trunks and rocks) within a three-meter radius area [21]. When palms were found within the sampling radius, manual searches were conducted by skilled personnel. Briefly, one skilled searcher used a ladder to perform the search from the base of the leaves. Manual searches for triatomines were carried out within the foliage. Any nest found was carefully extracted and dissected on a 2 m2 yellow plastic sheet placed at the base of the palm. Underground mammal burrows were searched by extracting all material found within placed onto a yellow plastic sheet. The total area sampled on each occasion was 1981 m2. In each microhabitat, we recorded information regarding habitat type (forest, shrub, or crop; see below), occurrence of vertebrate hosts (identified to species level based on nest occurrence), and nest height from ground level. At each date, sampling lasted for 5 days, representing a total sampling effort of 840 person-hours for the whole study.

Landscape Features and Dynamics

About 50% of the total land cover of the studied community was composed of cultivated areas, mainly corn, papaya, and peanut crops. The rest of the landscape was vegetated with a lowland semideciduous forest composed of thorny plants and trees that lose their leaves once a year (e.g., Ceratonia siliqua L. 1858 and Guazuma ulmifolia Lam. 1789) [8]. In this region, there is only one harvest of short-cycle maize per year as there is not enough precipitation during the dry season and most farmers do not have irrigation facilities. Maize is generally planted during the rainy season and is harvested during the following transition period (May-June).

For the seven sampling periods, each of the 70 sampled points was assigned to a habitat type as follows: 1) forest, characterized by a high density of G. ulmifolia tree, 2) shrub, characterized by high density of Cordia lutea Lam. and 3) crops. While elaborated landscape analyses can be performed using indices describing the landscape based on the composition and disposition of its constituents [22], we realized that, at our study scale, a simple characterization of land cover modification over the whole year at each of the 490 triatomine sampling points (70 sampling points×7 months) and 12 houses was sufficient to describe the dynamics of this agricultural landscape [23].

Triatomine Identification and T. cruzi Analyses

Once collected, triatomines were placed in individually labeled plastic containers and transported to the Center for Infectious Disease Research (PUCE, Quito) where they were counted and identified to the species and instar level. Species identification was based on morphological criteria [24], [25] and comparisons with pinned specimens of the PUCE's Entomology Museum (QCAZ). The presence of T. cruzi-like organisms was determined by examination of the feces and intestinal content at ×400 magnification, using a direct light microscope and by PCR using S35/S36 and 121/122 primer sets [26], [27]. Discrete typing units (DTU) were determined as described in Ocaña-Mayorga et al. [28].

Data Analyses

Triatomine infestation.

Four entomological indices were calculated to describe triatomine infestation levels in both synanthropic and sylvatic environments over the 7 sampling dates: (1) infestation index (number of positive houses or sylvatic collection point/number of houses or sylvatic collection points examined×100), (2) density (number of triatomines collected/number of houses or nests examined), (3) crowding (number of triatomines collected/number of positive houses or nests), and (4) colonization index (number of houses or nests with nymphs/number of positive houses or nests×100) [11], [29].

Triatomine density interpolation mapping.

To visualize the spatio-temporal dynamics of triatomine abundance in the studied community we built density interpolation maps using information on the number of triatomines collected at the coordinates of the 70-quadrat points at each visit. The data were adjusted at a surface z(x,y) form, by means of the Gridfit function of the Software MATLAB 7.0 [30]. Interpolations relating information between nearest neighbors were obtained to develop a Gridfit-modeled surface compiled with the plot of the sampled points (see Dangles et al. [31] for more details). These plots were overlapped with maps of synanthropic infestation generated in ArcMap 9.3 (ESRI Inc. 1999-2008).

Multivariate and path analyses.

We used ordination techniques coupled with path analyses to examine the direct and indirect effects of season (reflected by precipitation levels) on T. cruzi-infected abundance. First, in order to test whether landscape changes over the year (i.e. 7 sampling dates) affected the distribution of triatomines, multivariate analysis of variance (MANOVA) and canonical variate analyses (CVA) were performed on habitat type variables (crop, shrub, forest) and rodent host variables (squirrel, rat and mouse) associated with the locations where triatomines were found. This analysis is analogous to a principal component of the main directions of variation among dates and allowed us to quantify the environmental spaces of infected triatomines and their temporal variation. Non-parametric MANOVA (10,000 permutations, p-values Bonferroni corrected) was performed on the Euclidean distances among pair of points [32]. The Wilks' k from the MANOVA represents the ratio between pooled within-groups and total covariance matrices. Values close to 1.0 indicate equality of covariance matrices within and among dates and, thus, no statistical differences among their centroids. CVA results were cross-validated using the leave-one-out procedure (Jackknife procedure) on the classifier matrix, where a concordance between classification success rates in the non-validated and validated analyses indicates that group discrimination was not based on a one-case contribution. All these analyses were run in PAST 2.0 using both total triatomine and T. cruzi-infected triatomine. As both analyses gave similar results, only those concerning infected triatomines are presented.

Second, we used path analysis via structural equation modeling as a powerful method exploring causal mechanisms that link triatomine abundance to environmental changes over one year [33]. Based on prior ecological knowledge on Ecuadorian triatomines in agricultural landscapes [8], [20], [21], [34], we used a relatively simple model in which the dependent variable was the abundance of T. cruzi-infected triatomine while the independent variable was season, expressed as rainfall. We used this model to assess how strong the season effect was on triatomine abundance and whether it was acting directly or indirectly through other factors such as habitat availability (either crop or shrubs and forest) and host occurrence (mice, rats and squirrels). More complex models were not used because sample size would not support them. All variables were transformed to have a normal distribution using z scores and log transformation prior to analysis. Path analyses were run in the sem package [35] for R, version 2.15.1 (R Development Core Team 2013).

Generalized linear model.

Generalized linear model analyses (GLM) were further used to test the effect of specific environmental variables on the density of triatomines. The variables included in the Poisson log-linear models were: date, habitats (forest, shrub, and crop), host (mouse, rat and squirrel), nest height, and the number of houses sprayed at the preceding sampling date. To avoid multicollinearity, some of the environmental variables that were highly correlated (| r |>0.7) (e.g. nest height and host) were not included in the same model. We also included a "collection point" variable to allow within-collection point comparisons while controlling for variation resulting from unmeasured collection point-specific parameters. Site was treated as fixed effects, but we obtained similar results by means of the generalized linear mixed model function (glmmPQL; MASS library for R), with sites as random effects. Change in triatomine densities due to each factor was modeled considering each factor independently and in combination with other factors, including biologically reasonable two-way interactions, and squared variables. The more parsimonious model was identified using the Akaike's Information Criterion (AIC, see [36]) in likelihood ratio tests to find the difference between the initial model and the reduced model, dropping an "effect" term. All analyses were performed on log-transformed data in R [37] using both total triatomine and T. cruzi-infected triatomine. As both analyses gave similar results, only those concerning infected triatomines are presented.

Results

A total of 886 triatomine specimens were collected over the seven sampling dates. Ninety nine percent of all collected specimens were Rhodnius ecuadoriensis Lent & León, 1958. The remaining five specimens were Panstrongylus howardi Neiva 1911 (3 individuals) collected in a squirrel nest in June 2009, one, P. geniculatus Latreille 1811 (1 individual) collected in the peridomicile in June 2009 and one P. rufotuberculatus collected in a squirrel nest in December 2009. Of the 886 specimens, 69% were collected in sylvatic habitats. As a general pattern, we collected a high number of first instar nymphs but few adults of R. ecuadoriensis in both sylvatic and synanthropic habitats. The majority (78.1%) of sylvatic triatomines were found in animal nests. These nests were either of squirrel (Sciurus stramineus Eydoux & Souleyet 1841, 83.1%), mouse/rat (10.9% e.g Sigmodon sp, Proechimys sp, Rhipidomys sp, Mus musculus L.,), bird (4.9%, e.g., Campylorhynchus fasciatus Swainson 1837) or other species (e.g., the opossum Didelphis marsupialis L. 1858, 1.1%). While triatomines were commonly found in squirrel nests all year round, they were associated with mouse/rat nests mostly during the rainy and transition seasons. Triatomines were not found in an abandoned structure, which was the only bat habitat found within the quadrat.

The infestation index ranged from 4.3% to 22.9% and 0% to 41.7% in sylvatic and synanthropic habitats, respectively (Table 1). The density index was generally lower in sylvatic than synanthropic habitats (except in April and June 2010) while the crowding index greatly varied among dates in both habitats. Colonization indices were always >85% in both habitats (except in December, April and June in houses). As a general pattern, the value of the three first entomological indices tended to be low for the last sampling dates (February to June 2010). This tendency was consistent with lower triatomine abundances and number of infested points found at the end of the sampling period (see Figure 2). Overall, half of the 12 sampled houses presented triatomine infestation at least once during the year-long survey. Of those, five houses had multiple infestations at different time points (Table S1).

[Figure omitted, see PDF]

Figure 2. Box-plot representation of abundance of triatomines expressed as the number of individuals collected by collection point in both sylvatic and domestic habitats for the 7 sampling dates.

Number above/below the box-plots, indicate the number of infested points. Box-plots were built using data from the 70 sampled sylvatic points in the quadrat and the seven infested houses (see Table S1).

Table 1. Entomological indices of Triatomine (R. ecuadoriensis) infestation calculated for both synanthropic (domestic-peridomestic, 12 DUs at each visit) and sylvatic habitats (70 sampling point at each visit) over the seven field visits.

Overall, triatomine abundance was highly variable among collection points at a given date (see Figure 2), resulting in the observed aggregated pattern of triatomine spatial distribution (Figure 3). Interestingly, these "hotspots" of triatomine density were located both nearby (<50 m) and relatively far (>200 m) from the houses.

[Figure omitted, see PDF]

Figure 3. Triatomine density interpolation maps of the study community for the 7 sampling dates.

Maps are based on data from both sylvatic and domestic habitats. Black lines represent principal and secondary roads in the community. Circles indicate houses that were visited. Orange stars represent collection points where triatomines infected by T. cruzi were sampled.

Infections with Trypanosoma spp were detected by PCR in 90% of the 105 sylvatic triatomines analyzed. Infections with T. cruzi were the most common (71%) followed by infections with T. rangeli (15%) and mixed infections T. cruzi/T. rangeli (4 cases, 4%). Infection rates were lower for individuals sampled in houses (60%, N = 55) with a large predominance of T. cruzi infections (58%), the remaining being infected by T. rangeli. The prevalence of triatomines' infection for each date and habitat is given in Figure 4.

[Figure omitted, see PDF]

Figure 4. Prevalence of triatomines' infection by T. cruzi, T. rangeli and mixed infections for each sampling date and habitat.

X axis labels correspond to C = crops, F = forest, S = shrub and H = House.

Multivariate analyses (MANOVA and CVA) showed that the environmental spaces of T. cruzi-infected triatomines were significantly different among dates (Wilks' k from MANOVA = 0.119; P<0.001). The first canonical variate (axis 1), primarily associated with crop and mice, explained 64.7% of the variability among dates and clearly discriminated the environmental spaces from dry (August-October) to rainy season (April; Figure 5). The second canonical variate (axis 2, 23.7%) was primarily associated with shrubs and tended to separate the transition season following the dry season (December) from the transition season following the rainy season (June; Figure 5). Note that May-June 2009 experienced unusually low precipitation, which explains the position of its environmental space together with August and October. Path analyses further suggested that rainfall significantly determined the abundance of T. cruzi-infected triatomines via indirect paths involving habitats and rodent hosts (Figure 6). Rainfall had a significantly positive and negative influence on crop and forest/shrub cover, respectively. In turn, these habitats significantly influence the occurrence of rodent hosts and thereby the abundance of T. cruzi-infected triatomines.

[Figure omitted, see PDF]

Figure 5. Canonical ordination of the environmental variables associated with the presence of T. cruzi-infected triatomines for the different sampling dates between June 2009 and June 2010.

Polygons represent the "environmental spaces" (see main text) of infected triatomines at each date. The first canonical axis explains 64.7% of the variation among groups.

[Figure omitted, see PDF]

Figure 6. Path diagram resulting from structural equation modelling of factors affecting populations of T. cruzi-infected triatomines in Manabi, Coastal Ecuador (Bentler CFI = 0.91).

Path coefficient and significance (* P≤0.05; ** P≤0.01; *** P≤0.001) are given above each arrow. Arrow thickness is scaled by the standardized loading coefficients for each connection. Dashed arrows are non significant.

Of the five factors included in the GLM analysis (see Materials and Methods) three of them, date, habitat, and host, significantly affected the abundance of infected triatomines with 'habitat' and 'date' showing the highest ΔAIC values (Table 2). Moreover, the significant 'date×habitat' interaction term revealed that changes in habitat structure over the year influenced significantly the abundance of infected triatomines. We also found a significant 'date×vector' interaction term which supports that changes in rodent host populations affect the distribution of T. cruzi-infected triatomine through time. Note that similar results were found when performing the analyses with all triatomine data (infected and non-infected, Table S2).

Table 2. Results of the generalised linear model deviance analysis on the number N of triatomines (R. ecuadoriensis) infected by T. cruzi.

Discussion

Our study confirms the prominent role of sylvatic triatomines in Chagas disease epidemiology due to their very high infection rates by T. cruzi (71%). We also provide strong evidence that environmental spaces of triatomines (either infected or not) varied predictably over the year due to changes in land cover (in particular the cultivated-natural habitat switch) and associated occurrence of rodent hosts. This highlights the importance of both environmental and sociological factors (e.g., agricultural scheduling) in shaping the spatio-temporal population dynamics of triatomines.

Triatomine Spatio-temporal Dynamics

Our study reports that the species R. ecuadoriensis, the most important Chagas disease vector in coastal Ecuador, has well established T. cruzi-infected sylvatic populations all year round. Although the occurrence of these vectors in Ecuador is well known [8], [34], [38], no detailed data on their temporal dynamics were available. Our findings confirm that the demographic structure of sylvatic R. ecuadoriensis population was dominated by nymphal stages over the year [8] and that aggregations occurred both in sylvatic habitats and closer to homes [8], [39]. In agricultural landscapes such as coastal Ecuador, the impact of human activities through crop planting and deforestation can extend far beyond the close periphery of households, which may explain the presence of triatomine aggregation areas relatively far from populated areas [40], [41]. As previously reported by [14], these results emphasize the importance of sylvatic populations for the transmission cycle of T. cruzi. Currently there are no genetic markers available to conduct population genetic studies that compare populations of R. ecuadoriensis collected in houses and sylvatic environments. However, morphometric analyses using antennae sencilla and wing geometry failed to detect significant differences between these populations [42].

As found in other studies (e.g., Mexico [23], USA [43]), triatomines were strongly associated with squirrels which probably represent one of the most important host species [21], [34]. The relatively large body size of squirrels and the characteristics of their nests (large, 30 cm diameter, loose construction, and usually located at >5 m from the ground) could improve food source availability and protection for triatomines [8]. The spatio-temporal dynamics of triatomine bugs in our study area was therefore highly influenced by variations in the occurrence of squirrels over the year and their distribution among the different habitat types (see below). These results confirm a previous study by Gottdenker et al. [12], [13] who found that changing host community structure following anthropogenic landscape disturbance in Panama may increase vector infection with T. cruzi. These authors further showed the importance of host reproductive rates as an important determinant of vector infection, suggesting that further research on rodent host life history in coastal Ecuador would be needed to improve our knowledge on T. cruzi epidemiology.

T. cruzi-Infected Sylvatic Triatomine Cycle

Several studies have been performed to characterize and correlate sylvatic and synanthropic habitats with triatomine presence/absence using environmental parameters such as climate and topography [44], [45], associations with mammal hosts [16], [46], birds and palm trees [9], [47]; (Grijalva unpublished data), or specific land-cover types [48]. As revealed by previous studies from other parts of Latin America [12]-[16], the spatio-temporal distribution cycle of R. ecuadoriensis in coastal Ecuador comes as a result of a combination of multiple ecological factors. Because climatic conditions of the Ecuadorian Coast are favorable for triatomine development all year round (all months are moist and warm), land cover features represent the key variables that shape the population dynamics of T. cruzi vectors on a local scale [8]. Moreover, in view of the importance of agricultural activities in coastal Ecuador, land cover patterns and dynamics are likely influenced by land use practices of rural communities [11].

Our quantitative results from both the MANOVA-CVA, the path analysis and the GLM analyses (themselves based on an extensive sampling of 886 triatomine individuals and associated micro-environmental parameters over one year) strongly suggest that variations in land cover over one year, and subsequent effect on the distribution of rodent hosts, drive R. ecuadoriensis population dynamics in coastal Ecuador. While, for logistical reasons, our sampling was limited to one year in one location, results found are in agreement with other studies performed by our team over the last 10 years in other regions of Ecuador at different periods of the year (e.g. [8], [11], [20], [34], [38]), reinforcing their general relevance. Based on this accumulated knowledge, we propose a schematic framework identifying the factors affecting the distribution of sylvatic T. cruzi vectors over one year (Figure 7). During the rainy season (January-April), triatomines are mainly associated with squirrels whose nests are abundant in leafy bushes, hidden from predators. Some triatomines are also found in mouse nests in growing crops. A few months later, crops mature and food availability increases the association of triatomine with mice/rats, which become important hosts for them. During the dry season (July-October) triatomines mainly colonize squirrel nests located in trees. As many tree and shrub species lose their leaves at this season, it may be more secure for squirrels to nest high in the trees, generally >5 m, [8] than in shrubs. Finally, during the transition period that follows the dry season, triatomines are only found at low densities in squirrel nests associated with tree and leafing bushes. This season seems to be the most critical for the survival of sylvatic triatomines due to poor resource conditions in term of both habitat and hosts. Overall, land cover changes due to both farming activities and vegetation phenology affect rodent host distribution and, consequently, that of triatomines. Such findings extend to agricultural systems the results of previous studies in forested habitats that have shown that the composition and relative abundance of small mammal fauna involved in the transmission cycle of Chagas disease can be influenced by landscape structure and its changes over the year [16].

[Figure omitted, see PDF]

Figure 7. Cycle of infected sylvatic triatomines in coastal Ecuador resulting from changes in land cover (crop, forest, and shrub) and associated host distribution (mice and squirrels).

The relative size of land cover and animal icons refers to the relative importance of these factors. Rainy period: Jan-Apr, Transition period 1: May-June, Dry period: July-Oct., Transition period 2 = Nov.-Dec.

Still, causal mechanisms that link triatomine abundance to seasonal environmental changes are poorly understood due to the difficulty in performing large-scale landscape manipulation experiments and evaluating contributions from direct and indirect factors that can have opposing effects on triatomine populations [13]. However, our path analysis helped to stress the importance of indirect effects from field data. The triatomine population studied here clearly responded primarily to local habitat and host availability changes, which were changing over the year due to differences in precipitation. Additional work is needed to fully understand the epidemiological cycle of T. cruzi in changing landscapes. Our path analysis suggests individual hypotheses that can be tested experimentally or further investigated with more detailed observations as each arrow or path identifies a putative causal relationship between the triatomine populations and seasonality-driven environmental aspects.

Implications for Control

The burden of endemic neglected zoonoses generally falls heavily on rural communities with limited resources. In Ecuador, rural communities with subsistence-farming practices are high-risk areas for acquiring Chagas disease [20]. As control of Chagas disease should rely on the interruption of parasite transmission to domestic hosts [8], a sound understanding of infection risk factors in both sylvatic and synanthropic vector populations is needed to effectively assist the development of effective prevention programs [49]. In this context, the results of this study have three major implications. First, our study emphasizes the importance of sylvatic triatomine populations as a main component of the transmission cycle of T. cruzi. While insecticide spraying in houses have allowed the control of domestic individuals (see Figure 2), numerous sylvatic individuals remain in the vicinity of households, which constitute a potential source of vectors for re-colonization [11]. Second, while there is a decline in the number of individuals over time, our results corroborate the previously reported low effectiveness of deltamethrin and suggest a short lasting residual effect of deltamethrin in the environmental conditions of coastal Ecuador. This has been observed in other areas such as the Chaco region [50]. Third, our findings highlight the importance of both environmental and sociological (farming practices) factors in shaping the spatio-temporal population dynamics of T. cruzi vectors. We propose that further research on T. cruzi transmission cycles should follow a social-ecological approach (e.g. using methods developed for the study of socio-ecological systems, such as agent-based models, [51]), in which the coupling of human and natural systems would reveal the complex patterns and processes emerging from their interactions [52]. For example, if farming communities of coastal Ecuador would develop irrigation to support cultivation of maize during the dry season, this may have an impact on the sylvatic triatomine cycle through an increase of mouse/rat and squirrel occurrence during the dry and following transition seasons. However, two maize harvests per year would boost farmers' income and probably improve their living conditions, thereby decreasing their vulnerability to Chagas disease [1] and potentially reducing T. cruzi transmission [53]. Indeed, even low cost home improvements that limit areas of vector refuge in nearby houses can be highly effective at keeping infestation low [54]. Understanding such complex effects of land use changes on T. cruzi transmission and overall potential negative and/or positive feedback between farming practices, habitat availability, hosts and vectors would provide crucial, yet poorly considered, information with strong implications for vector surveillance and control.

Supporting Information

Table S1.

Number of triatomines per house collected in the 12 sampled individual houses within the quadrat over the study period (June 2009-June 2010).

(DOCX)

Table S2.

Results of the generalised linear model deviance analysis on the total number N of triatomines (R. ecuadoriensis). AIC = Akaike's information criterion of the initial model after the removal of the 'effect' term.

(DOCX)

Acknowledgments

The authors are grateful to people of visited communities and entomologists from the Ecuadorian National Chagas Control Program for their helpful collaboration. They also thank Anita G. Villacís, Sofia Ocaña-Mayorga, Sofía Muñoz, César A. Yumiseva, and Esteban G. Baus for their technical assistance, and Frédérique Brénière, Claudio Lazzari, Ana Lucia Moncayo, Sofia Ocaña-Mayorga, Anita Villacís and three anonymous reviewers for the critical review of the manuscript.

Author Contributions

Conceived and designed the experiments: MJG DT OD. Performed the experiments: DT OD. Analyzed the data: DT OD. Contributed reagents/materials/analysis tools: MJG DT OD. Wrote the paper: MJG DT OD.

References

Hotez PJ, Dumonteil E, Woc-Colburn L, Serpa JA, Bezek S, et al. (2012) Chagas disease: The New HIV/AIDS of the Americas. PLOS Negl Trop Dis 6: e1498. doi: 10.1371/journal.pntd.0001498

Tanowitz HB, Weiss LM, Montgomery SP (2011) Chagas disease has now gone global. PLOS Negl Trop Dis 5: e1136. doi: 10.1371/journal.pntd.0001136

Leite GR, dos Santos CB, Falqueto A (2011) Influence of the landscape on dispersal of sylvatic triatomines to anthropic habitats in the Atlantic Forest. J Biogeogr 38: 651-663. doi: 10.1111/j.1365-2699.2010.02442.x

Gurgel-Goncalves R, Galvao C, Costa J, Peterson A (2012) Geographic distribution of Chagas disease vectors in Brazil based on ecological niche modeling. J Trop Med 2012: 705326. doi: 10.1155/2012/705326

Zeledon R, Rabinovich JE (1981) Chagas disease - an ecological appraisal with special emphasis on its insect vectors. Annu Rev Entomol 26: 101-133. doi: 10.1146/annurev.en.26.010181.000533

Guhl F, Jaramillo C, Vallejo GA, Cardenas AAF, Aufderheide A (2000) Chagas disease and human migration. Mem Inst Oswaldo Cruz 95: 553-555. doi: 10.1590/s0074-02762000000400018

Noireau F, Bosseno MF, Carrasco R, Telleria J, Vargas F, et al. (1995) Sylvatic triatomines (Hemiptera, Reduviidae) in Bolivia - trends toward domesticity and possible infection with Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae). J Med Entomol 32: 594-598

Suarez-Davalos V, Dangles O, Villacis AG, Grijalva MJ (2010) Microdistribution of sylvatic triatomine populations in central-coastal Ecuador. J Med Entomol 47: 80-88. doi: 10.1603/033.047.0111

Abad-Franch F, Palomeque FS, Aguilar HM, Miles MA (2005) Field ecology of sylvatic Rhodnius populations (Heteroptera, Triatominae): risk factors for palm tree infestation in western Ecuador. Trop Med Int Health 10: 1258-1266. doi: 10.1111/j.1365-3156.2005.01511.x

Ceballos LA, Piccinali RV, Marcet PL, Vazquez-Prokopec GM, Cardinal MV, et al. (2011) Hidden sylvatic foci of the main vector of Chagas disease Triatoma infestans: threats to the vector elimination campaign? PLOS Negl Trop Dis 5: e1365. doi: 10.1371/journal.pntd.0001365

Grijalva MJ, Villacis AG, Ocana-Mayorga S, Yumiseva CA, Baus EG (2011) Limitations of selective deltamethrin application for triatomine control in central coastal Ecuador. Parasit Vectors 4: 20. doi: 10.1186/1756-3305-4-20

Gottdenker NL, Calzada JE, Saldana A, Carroll CR (2011) Association of anthropogenic land use change and increased abundance of the Chagas disease vector Rhodnius pallescens in a rural landscape of Panama. Am J Trop Med Hyg 84: 70-77. doi: 10.4269/ajtmh.2011.10-0041

Gottdenker NL, Chaves LF, Calzada JE, Saldana A, Carroll CR (2012) Host life history strategy, species diversity, and habitat influence Trypanosoma cruzi vector infection in changing landscapes. PLOS Negl Trop Dis 6: e1884. doi: 10.1371/journal.pntd.0001884

Ramsey JM, Gutierrez-Cabrera AE, Salgado-Ramirez L, Peterson AT, Sanchez-Cordero V, et al. (2012) Ecological connectivity of Trypanosoma cruzi reservoirs and Triatoma pallidipennis hosts in an anthropogenic landscape with endemic Chagas disease. PLOS One 7: e46013. doi: 10.1371/journal.pone.0046013

Herrera HM, Rocha FL, Lisboa CV, Rademaker V, Mourao GM, et al. (2011) Food web connections and the transmission cycles of Trypanosoma cruzi and Trypanosoma evansi (Kinetoplastida, Trypanosomatidae) in the Pantanal Region, Brazil. Trans R Soc Trop Med Hyg 105: 380-387. doi: 10.1016/j.trstmh.2011.04.008

Herrera HM, Rademaker V, Abreu UGP, D'Andrea PS, Jansen AM (2007) Variables that modulate the spatial distribution of Trypanosoma cruzi and Trypanosoma evansi in the Brazilian Pantanal. Acta Trop 102: 55-62. doi: 10.1016/j.actatropica.2007.03.001

Hassell M (2000) The spatial and temporal dynamics of host-parasitoid interactions. Oxford: Oxford University Press. viii, 200 pp

Pan American Health Organization - PAHO (2006) Technical Report: Quantitative Chagas disease estimates: Pan American Health Organization. OPS/HDM/CD/425-06. 28 p

Black CL, Ocana S, Riner D, Costales JA, Lascano MS, et al. (2007) Household risk factors for Trypanosoma cruzi seropositivity in two geographic regions of Ecuador. J Parasitol 93: 12-16. doi: 10.1645/ge-899r.1

Grijalva MJ, Palomeque-Rodriguez FS, Costales JA, Davila S, Arcos-Teran L (2005) High household infestation rates by synanthropic vectors of Chagas disease in southern Ecuador. J Med Entomol 42: 68-74. doi: 10.1603/0022-2585(2005)042[0068:hhirbs]2.0.co;2

Grijalva MJ, Villacis AG (2009) Presence of Rhodnius ecuadoriensis in sylvatic habitats in the southern highlands (Loja Province) of Ecuador. J Med Entomol 46: 708-711. doi: 10.1603/033.046.0339

Turner MG (2005) Landscape ecology: What is the state of the science? Annu Rev Ecol Evol Syst 36: 319-344. doi: 10.1146/annurev.ecolsys.36.102003.152614

Ramsey JM, Alvear AL, Ordonez R, Munox G, Garcia A, et al. (2005) Risk factors associated with house infestation by the Chagas disease vector Triatoma pallidipennis in Cuernavaca metropolitan area, Mexico. Med Vet Entomol 19: 219-228. doi: 10.1111/j.0269-283x.2005.00563.x

Lent HWP (1979) Revision of the Triatominae (Hemiptera: Reduviidae), and their significance as vectors of Chagas disease. Bull Am Mus Nat Hist 163: 123-520

Carcavallo RU, Galíndez-Giron I, Jurberg J, Galvao C, Lent H (1998) Pictorial keys for tribes, genera and species of the subfamily Triatominae. In: Carcavallo RU, Galíndez-Giron I, Jurberg J, Lent H, editors. Atlas of Chagas' Disease Vectors in the Americas. Rio de Janeiro: Editora Fiocruz. pp. 107-244

Vallejo GA, Guhl F, Chiari E, Macedo AM (1999) Species specific detection of Trypanosoma cruzi and Trypanosoma rangeli in vector and mammalian hosts by polymerase chain reaction amplification of kinetoplast minicircle DNA. Acta Trop 72: 203-212. doi: 10.1016/s0001-706x(98)00085-0

Virreira M, Alonso-Vega C, Solano M, Jijena J, Brutus L, et al. (2006) Congenital Chagas disease in Bolivia is not associated with DNA polymorphism of Trypanosoma cruzi. Am J Trop Med Hyg 75: 871-879

Ocana-Mayorga S, Llewellyn MS, Costales JA, Miles MA, Grijalva MJ (2010) Sex, subdivision, and domestic dispersal of Trypanosoma cruzi lineage I in southern Ecuador. PLOS Negl Trop Dis 4: e915. doi: 10.1371/journal.pntd.0000915

World Health Organization - WHO (2002) Control of Chagas disease: Second Report of the WHO Expert Committee: World Health Organization. i-vi, 1-109

D'Errico JR (2006) Understanding Gridfit. Available: http://www.mathworks.com/matlabcentral/fileexchange/8998-surface-fitting-using-gridfit

Dangles O, Carpio C, Barragan AR, Zeddam JL, Silvain JF (2008) Temperature as a key driver of ecological sorting among invasive pest species in the tropical Andes. Ecol Appl 18: 1795-1809. doi: 10.1890/07-1638.1

Hammer O, Harper DAT, Ryan PD (2001) PAST: Paleontological statistics software package for education and data analysis. Palaeontol Electron 4: 9

Shipley B (2000) Cause and correlation in biology: a user's guide to path analysis, structural equations, and causal inference. Cambridge, UK: Cambridge University Press. 317 pp

Grijalva MJ, Suarez-Davalos V, Villacis AG, Ocana-Mayorga S, Dangles O (2012) Ecological factors related to the widespread distribution of sylvatic Rhodnius ecuadoriensis populations in southern Ecuador. Parasit Vectors 5: 17. doi: 10.1186/1756-3305-5-17

Fox J, Bymes J (2011) SEM: structural equation models. R package, version 2.1-1. Available: http://CRAN.R-project.org/packagepsem

Venables WN, Ripley BD, Venables WN (2002) Modern applied statistics with S. New York: Springer. 495 pp

R-Development-Core-Team (2012) R. A language and environment for statistical computing. Foundation for statistical computing Available: http://web.mit.edu/r_v3.0.1/fullrefman.pdf

Grijalva MJ, Palomeque FS, Villacis AG, Black CL, Arcos-Teran L (2010) Absence of domestic triatomine colonies in an area of the coastal region of Ecuador where Chagas disease is endemic. Mem Inst Oswaldo Cruz 105: 677-681. doi: 10.1590/s0074-02762010000500013

Vazquez-Prokopec GM, Cecere MC, Canale DM, Gurtler RE, Kitron U (2005) Spatiotemporal patterns of reinfestation by Triatoma guasayana (Hemiptera: Reduviidae) in a rural community of northwestern Argentina. J Med Entomol 42: 571-581. doi: 10.1603/0022-2585(2005)042[0571:sporbt]2.0.co;2

Jansen AM, de Pinho APS, Lisboa CV, Cupolillo E, Mangia RH, et al. (1999) The sylvatic cycle of Trypanosoma cruzi: A still unsolved puzzle. Mem Inst Oswaldo Cruz 94: 203-204. doi: 10.1590/s0074-02761999000700030

Ramirez-Sierra MJ, Herrera-Aguilar M, Gourbiere S, Dumonteil E (2010) Patterns of house infestation dynamics by non-domiciliated Triatoma dimidiata reveal a spatial gradient of infestation in rural villages and potential insect manipulation by Trypanosoma cruzi. Trop Med Int Health 15: 77-86. doi: 10.1111/j.1365-3156.2009.02422.x

Villacis AG, Grijalva MJ, Catala SS (2010) Phenotypic variability of Rhodnius ecuadoriensis populations at the Ecuadorian central and southern Andean region. J Med Entomol 47: 1034-1043. doi: 10.1603/me10053

Navin TR, Roberto RR, Juranek DD, Limpakarnjanarat K, Mortenson EW, et al. (1985) Human and sylvatic Trypanosoma cruzi infection in California. Am J Public Health 75: 366-369. doi: 10.2105/ajph.75.4.366

Costa J, Peterson AT, Beard CB (2002) Ecologic niche modeling and differentiation of populations of Triatoma brasiliensis Neiva 1911, the most important Chagas' disease vector in northeastern Brazil (Hemiptera, Reduviidae, Triatominae). Am J Trop Med Hyg 67: 516-520

Dumonteil E, Gourbiere S (2004) Predicting Triatoma dimidiata abundance and infection rate: A risk map for natural transmission of Chagas disease in the Yucatan Peninsula of Mexico. Am J Trop Med Hyg 70: 514-519

Peterson AT, Sanchez-Cordero V, Ben Beard C, Ramsey JM (2002) Ecologic niche modeling and potential reservoirs for Chagas disease, Mexico. Emerg Infect Dis 8: 662-667. doi: 10.3201/eid0807.010454

Gurgel-Goncalves R, Cuba CAC (2009) Predicting the potential geographical distribution of Rhodnius neglectus (Hemiptera, Reduviidae) based on ecological niche modeling. J Med Entomol 46: 952-960. doi: 10.1603/033.046.0430

Ibarra-Cerdena CN, Sanchez-Cordero V, Peterson AT, Ramsey JM (2009) Ecology of North American Triatominae. Acta Trop 110: 178-186. doi: 10.1016/j.actatropica.2008.11.012

Lannes-Vieira J, de Araujo-Jorge TC, Soeiro MDC, Gadelha P, Correa-Oliveira R (2010) The Centennial of the discovery of Chagas disease: facing the current challenges. PLOS Negl Trop Dis 4: e645. doi: 10.1371/journal.pntd.0000645

Gurtler RE, Canale DM, Spillmann C, Stariolo R, Salomon OD, et al. (2004) Effectiveness of residual spraying of peridomestic ecotopes with deltamethrin and permethrin on Triatoma infestans in rural western Argentina: a district-wide randomized trial. Bull World Health Organ 82: 196-205

Rebaudo F, Dangles O (2011) Coupled information diffusion-pest dynamics models predict delayed benefits of farmer cooperation in pest management programs. PLOS Comput Biol 7: e1002222. doi: 10.1371/journal.pcbi.1002222

Liu JG, Dietz T, Carpenter SR, Alberti M, Folke C, et al. (2007) Complexity of coupled human and natural systems. Science 317: 1513-1516. doi: 10.1126/science.1144004

Moreno ML, Hoyos L, Cabido M, Catala SS, Gorla DE (2012) Exploring the association between Trypanosoma cruzi infection in rural communities and environmental changes in the southern Gran Chaco. Mem Inst Oswaldo Cruz 107: 231-237. doi: 10.1590/s0074-02762012000200013

Lucero DE, Morrissey LA, Rizzo DM, Rodas A, Garnica R, et al. (2013) Ecohealth interventions limit triatomine reinfestation following insecticide spraying in La Brea, Guatemala. Am J Trop Med Hyg 88: 630-637. doi: 10.4269/ajtmh.12-0448