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Appl Biochem Biotechnol (2012) 168:11011107
DOI 10.1007/s12010-012-9844-z
Farshad Darvishi & Jacqueline Destain & Iraj Nahvi & Philippe Thonart & Hamid Zarkesh-Esfahani
Received: 12 November 2011 /Accepted: 14 August 2012 /
Published online: 30 August 2012# Springer Science+Business Media, LLC 2012
Abstract The extracellular lipase of Yarrowia lipolytica presents numerous potentialities for biotechnological applications. This work describes the development and storage of powders obtained from supernatants containing Y. lipolytica lipase by freeze-drying as downstream process that is important in obtaining a stable lipase powder with high enzymatic activity. Lipase was produced by Y. lipolytica U6 mutant strain in 20-L bioreactor. Non-concentrated cell-free culture supernatant samples were supplemented with different concentrations (0.51 %) of maltodextrin and glycerol as additives to freeze-drying. Effects of additives, temperature, pH, and storage time on lipase powders were determined. After addition of additives, freeze-drying yield increased 3.5-fold compared to supernatant without additive. Maltodextrin with0.5 % concentration gave the best protection of lipase during dehydration treatment and its freeze-drying yield (77 %) is better than other formulations. Lipase powders were stored at 4 and 25 C for 46 weeks without loss of lipase activity. A common impediment to the production of commercial enzyme is their low-stability aqueous solutions. The present study shows that freeze-dried lipase powders of Y. lipolytica have good stability for storage and various applications.
Keywords Lipase . Yarrowia lipolytica . Downstream processing . Formulation . Freeze-drying
F. Darvishi (*)
Department of Microbiology, Faculty of Science, University of Maragheh, Maragheh 55181-83111, Iran e-mail: [email protected]
I. Nahvi : H. Zarkesh-Esfahani
Department of Biology, Faculty of Science, University of Isfahan, Isfahan 81746-73696, Iran
J. Destain : P. Thonart
Centre Wallon de Biologie Industrielle, Unit de Bio-industries, Universit de Lige, Gembloux Agro-Bio Tech, Passage des Dports 2, 5030 Gembloux, Belgium
Effect of Additives on Freeze-Drying and Storage of Yarrowia lipolytica Lipase
1102 Appl Biochem Biotechnol (2012) 168:11011107
Introduction
Lipases (EC 3.1.1.3) are one of the most important classes of industrial enzymes. This class of enzymes presents a broad range of biotechnological applications. Therefore, interest to lipases has been greatly increased in recent years [1]. Extracellular lipase of Yarrowia lipolytica has many potential applications for the food industry, pharmacology, and environment [24]. Enantioselectivity characteristic of Y. lipolyticas lipase could be used to produce enantiomerically pure...