Effect of Ethanol Extract Sonchus arvensis Linn Leaf on BUN, SCr, ALT, AST in Healthy Male Albino mice (Rattus norvegicus).

Abstract

Sonchus leaves are traditional plants whose effects have been widely studied including immunostimulants and diuretics. Both of these effects have benefits for maintaining health and treating diseases. The purpose of this study was to determine the effect of ethanol extract sonchus on levels of BUN, SCr, ALT, AST and acute toxic effects on healthy male albino mice after being extracted 1 time a day for 14 days. Each group of test animals was given a dose of 100 mg/KgBW (n=9) for the BUN, SCr, ALT, AST test and every 10 healthy male albino mice (n=40) with a dose of 700, 1400, 2800, 5600 (mg/KgBW) for acute toxicity test. Blood sampling performed on day 0 and day 14 (BUN, SCr, ALT, AST) and toxicity tests were observed for 14 days. The data obtained were statistically tested with Shapiro Wilk normality test, or Wilcoxon T test and one way Annova or Friedman test and calculated LD50. Statistics show that there were no significant differences (p>0.005) in the administration of extracts between day 0 and day 14 of BUN, SCr, and AST levels but for ALT had significant difference (p<0.005). The results of this study concluded that the ethanol extract of sonchus leaves did not affect the levels of BUN, SCr, AST but neither for ALT on healthy male albino mice.