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J Membrane Biol (2013) 246:427433 DOI 10.1007/s00232-013-9541-z
ORIGINAL ARTICLES
The Effect of Glutathione Treatment on the Biochemical and Immunohistochemical Prole in Streptozotocin-Induced Diabetic Rats
Fatmagl Yur Semiha Dede Turan Karaca
Sevim ifti Yegin Yeter Deger Hlyazdemir
Received: 16 December 2012 / Accepted: 22 March 2013 / Published online: 17 May 2013 Springer Science+Business Media New York 2013
Abstract This study investigated the possible role of glutathione (GSH) in diabetic complications and its biochemical safety in experimental diabetic rats. Serum biochemical parameters and the histology of the pancreas were investigated. Seven rats were separated as controls. To create the diabetes in rats, 45 mg/kg single-dose streptozotocin (STZ) was administered i.p. The treatment was continued for 1 month. STZ was administered to the diabetes ? GSH group, then reduced GSH, dissolved in iso-tonic salt solution (200 mg/kg), was applied i.p. two times a week. The GSH group received i.p. GSH. Serum biochemical parameters were determined by autoanalyzer. Immunohistochemical procedures were used to determine the percentage of the insulin-immunoreactive b-cell area in the islets of Langerhans. The biochemical parameters changed to different degrees or did not change. Pancreatic cells of the control and GSH groups were healthy, but in the diabetic and GSH-treated diabetic groups we found damage in different numbers. The results from these analyses show that GSH supplementation can exert benecial effects on pancreatic cells in STZ-induced diabetic
rats and can safely be used for therapy in and protection from diabetes and complications of diabetes.
Keywords Biochemical parameter Diabetes
Glutathione Immunohistochemistry Rat
Introduction
Diabetes mellitus is a common illness with high morbidity and an early mortality rate, causing vascular, renal, retinal and neuropathic disorders in the long term as well as acute metabolic complications. Research has shown that long-term hyperglycemia causes adverse changes in blood vessels. Hyperglycemia, which occurs long before diagnosis, may cause organ damage. Therefore, early diagnosis is important for the treatment of patients with diabetes mellitus (Molitch 1990; Borodaco 2007; ADA 2011).
Glutathione (GSH) plays a protective role as an anti-oxidant defense system against reactive oxygen species (ROS)-mediated tissue damage. Diabetes causes a number of alterations in the antioxidant defense system (Lim et al. 2010). Alterations in its concentration have also been demonstrated to be a common feature of many pathological conditions including...