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J Mater Sci: Mater Med (2013) 24:12611269 DOI 10.1007/s10856-013-4892-7
Effect of nano-sized bioactive glass particles on the angiogenic properties of collagen based composites
Gabriela E. Vargas Luis A. Haro Durand Vanesa Cadena Marcela Romero
Rosa Vera Mesones Mirza Makovi Stefanie Spallek Erdmann Spiecker
Aldo R. Boccaccini Alejandro A. Gorustovich
Received: 11 September 2012 / Accepted: 10 February 2013 / Published online: 22 February 2013 Springer Science+Business Media New York 2013
Abstract Angiogenesis is essential for tissue regeneration and repair. A growing body of evidence shows that the use of bioactive glasses (BG) in biomaterial-based tissue engineering (TE) strategies may improve angiogenesis and induce increased vascularization in TE constructs. This work investigated the effect of adding nano-sized BG particles (n-BG) on the angiogenic properties of bovine type I collagen/n-BG composites. Nano-sized (2030 nm) BG particles of nominally 45S5 Bioglass composition were used to prepare composite lms, which were characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The in vivo angiogenic response was evaluated using the quail chorioallantoic membrane (CAM) as an model of angiogenesis. At 24 h post-implantation, 10 wt% n-BG containing collagen lms stimulated angiogenesis by increasing by 41 % the number of blood vessels branch points. In contrast, composite lms containing 20 wt% n-BG were found to inhibit angiogenesis. This experimental study provides
the rst evidence that addition of a limited concentration of n-BG (10 wt%) to collagen lms induces an early angiogenic response making selected collagen/n-BG composites attractive matrices for tissue engineering and regenerative medicine.
1 Introduction
Biodegradable composite systems incorporating nano-sized bioactive glasses (n-BG) have emerged recently as a new family of nano-structured biomaterials for biomedical applications [1]. The combination of bioactive glass nanoparticles or nanobers with biocompatible polymers, including polyesters such as poly(lactic acid), poly (hydroxybutyrate) and poly(caprolactone), and natural-based polymers such as proteins (collagen, silk broin) or polysaccharides (chitin, chitosan, starch) enables the production of nanocomposites with potential to be used in tissue engineering and regenerative medicine [13].
The physico-chemical, mechanical and biological advantages of incorporating n-BG in biodegradable nano-composites in comparison to conventional (micron-sized) bioactive glasses (l-BG) have been reviewed [1]. The use of n-BG is expected to improve both the mechanical and biological properties of polymeric matrices. The higher specic surface area of n-BG particles...