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OBJECTIVE: The purpose of this study was to determine the effects of glucosamine and celadrin on platelet function.
DESIGN: Baseline values were determined on the Chronolog 570VS platelet aggregometer with whole blood aggregation impedance readings using 2 different concentrations of ADP (5µ?, ??µ?), collagen (lpg/mL), arachidonic acid (0.5mM/L) and an Accumetrics whole blood platelet aggregation cartridge assay for P2Y12 receptors were obtained from 24 healthy volunteers. These subjects then took the suggested doses of Glucosamine with Celadrin (Stockbridge Naturals) as advertised (estimated 1500mg daily) for 2 weeks. Platelet aggregation analyses, as described above, were obtained after treatment. Statistics performed via a McNemar test.
MAIN OUTCOME: Five of twenty-four subjects had at least a 20% difference in whole blood aggregation using the 5µ? concentration of ADP. A total of 6 and 7 subjects also showed a significant difference in platelet aggregation with administration of collagen and arachidonic acid, respectively. No significant differences were found with Accumetrics assay for P2Y12 in any of the subjects.
CONCLUSION: Glucosamine and celadrin may inhibit platelet aggregation in some individuals via aspirin-like effects as well as inhibition of ADP receptor P2Y1 but not P2Y12.
ABBREVIATIONS: ADP = adenosine diphosphate, NSAID = non-steroidal anti-inflammatory drug, EDTA = ethylenediaminetetraacetic acid, WBA = whole blood aggregation, CBC = complete blood count, PGE = prostaglandin-E
INDEX TERMS: Blood platelets, Platelet aggregation, Glucosamine, Celadrin
Clin Lab Sci 2010;23(1):32
INTRODUCTION
The use of dietary supplements in the United States is on the rise. A national survey conducted by the Center for Disease Control in 20071 found that 17-7% of American adults had used "natural products" in the previous 12 months as compared to 2.1% in 1990.2 The most popular products used were fish oil/omega 3/DHA (37.4%), glucosamine (19.9%), and echinacea (19.8%).! Herbal and dietary supplements are not regulated by the Food and Drug Administration, dius raising concerns over potential side effects and drug interactions with conventional medicines. This use of supplements by adults suggested to our cohort that glucosamine and celadrin may affect platelet function.
One of our subject controls, who has donated blood as a normal platelet aggregometry control for years, suddenly had abnormal values suggesting platelet inhibition. Whole blood aggregation using 5uM and ??µ? concentrations of ADP showed 5O and 0O, respectively (...