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J Cancer Res Clin Oncol (2015) 141:6168 DOI 10.1007/s00432-014-1789-x
ORIGINAL ARTICLE CANCER RESEARCH
The efcacy of EGFR gene mutation testing in various samplesfrom nonsmall cell lung cancer patients: a multicenter retrospective study
Pawe Krawczyk Rodryg Ramlau Joanna ChorostowskaWynimko Tomasz Powrzek
Marzena Anna Lewandowska Janusz Limon Bartosz Wasag Juliusz Pankowski Jerzy Kozielski
Ewa KalinkaWarzocha Aleksandra Szczesna Kamila WojasKrawczyk Micha Skroski Rafa Dziadziuszko Paulina Jagu Ewelina Antoszewska Justyna Szumio Bozena Jarosz Aldona Woniak Wojciech Jwicki Wojciech Dyszkiewicz Monika PasiekaLis Dariusz M. Kowalski Maciej Krzakowski Jacek Jassem
Janusz Milanowski
Received: 18 June 2014 / Accepted: 24 July 2014 / Published online: 3 August 2014 The Author(s) 2014. This article is published with open access at Springerlink.com
Methods Deletions in exon 19 and substitution L858R in exon 21 of EGFR gene were assessed using real-time PCR techniques in 1,138 small biopsies or cytological specimens and in 1,312 surgical samples.
Results Out of 2,450 diagnostic samples (containing >10 % of tumor cells), the occurrence of EGFR gene mutations was 9 %; more frequently in women (13.9 %) and adenocarcinoma patients (10 %), particularly with accompanying expression of TTF1 (13.0 %). The frequency of EGFR gene mutations was similar in cytological and histological specimens, and in primary and metastatic lesions,
Abstract
Introduction Testing for the epidermal growth factor receptor (EGFR) gene mutations requires considerable multidisciplinary experience of clinicians (for appropriate patient selection), pathologists (for selection of appropriate cytological or histological material) and geneticists (for performing and reporting reliable molecular tests). We present our experience on the efcacy of routine EGFR testing in various types of tumor samples and the frequency of EGFR mutations in a large series of Polish non-small cell lung cancer (NSCLC) patients.
P. Krawczyk (*) T. Powrzek K. Wojas-Krawczyk J. Milanowski
Department of Pneumonology, Oncology and Allergology, Medical University of Lublin, Jaczewskiego 8 St., 20-954 Lublin, Polande-mail: [email protected]
R. Ramlau
E.J. Zeyland Wielkopolska Center of Pulmonology and Thoracic Surgery, Poznan, Poland
R. Ramlau W. Dyszkiewicz
Cardiothoracic Surgery Department, Poznan University of Medical Science, Poznan, Poland
J. Chorostowska-Wynimko M. Skroski P. Jagu
Department of Genetics and Clinical Immunology, National Institute of Tuberculosis and Lung Diseases, Warsaw, Poland
M. A. Lewandowska
Molecular Oncology and Genetics Units, Department of Tumor Pathology and Pathomorphology, Franciszek Lukaszczyk Oncology Center, Bydgoszcz, Poland
M. A....