Estimation of exposure to dietary acrylamide based on mercapturic acids level in urine of Polish women post partum and an assessment of health risk

Journal of Exposure Science and Environmental Epidemiology (2016) 26, 288295

2016 Nature America, Inc. All rights reserved 1559-0631/16

http://www.nature.com/jes

Web End =www.nature.com/jes

ORIGINAL ARTICLE

Estimation of exposure to dietary acrylamide based on mercapturic acids level in urine of Polish women post partum and an assessment of health risk

Hanna Mojska1, Iwona Gieleciska1, Aleksandra Zieliska2, Joanna Winiarek2 and Wodzimierz Sawicki2

We determined metabolites of acrylamide and glycidamide concentrations (AAMA and GAMA, respectively) in urine of 93 women within the rst days after delivery, using LC-MS/MS. The median AAMA and GAMA levels in urine were 20.9 g/l (2.3399.0 g/l) and8.6 g/l (1.385.0 g/l), respectively. In smokers we found signicantly (Po0.01) higher levels of metabolites in comparison with the non-smoking women. As demonstrated by the 24-h dietary recall, acrylamide intake was low (median: 7.04 g/day). Estimated exposure to acrylamide based on AAMA and GAMA levels in the whole group of women was 0.16 g/kg b.w./day(1.15 g/kg b.w./day, P95). We found signicantly (Po0.05) higher exposure in women who consumed higher amount of acrylamide in the diet (10 g/day vs o10 g/day). A weak but signicant positive correlation between acrylamide intake calculated on the basis of urinary levels of AAMA and GAMA and estimated on the basis of 24-h dietary recall (r = 0.26, Po0.05) was found. The estimated margin of exposure values were below 10 000 and ranged from 156 for 95th percentile to 1938 for median

acrylamide intake. Our results have shown that even a low dietary acrylamide intake may be associated with health risk.

Journal of Exposure Science and Environmental Epidemiology (2016) 26, 288295; doi:http://dx.doi.org/10.1038/jes.2015.12

Web End =10.1038/jes.2015.12 ; published online 1 April 2015

Keywords: acrylamide; urinary metabolites; dietary exposure; Polish women post partum; risk assessment; LC-MS/MS

INTRODUCTIONAcrylamide (AA) is a chemical compound extensively used in the industry for the production of polyacrylamide polymers. They are used as occulants to clarify drinking and industrial water, as gels for electrophoretic separation of proteins, in paper, cosmetic and textile industries. Increased interest in acrylamide has been noted since 2002, when the Swedish National Food Agency in collaboration with scientists from the Stockholm University1 published for the rst time data on the high content of acrylamide in high-carbohydrate products undergoing thermal processing. Acrylamide forms in food mainly as a result...