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ABSTRACT:
The present study was designed to evaluate the antioxidant and antimicrobial activity of various extracts of Buxus wallichiana Baill wood. Extraction of wood was done by hot continuous percolation process by using suitable solvents and the concentrated extract was used for the study. Anti oxidant Activity is done by in vitro DPPH (2,2-diphenyl-1- picryl hydrazyl ) method and Nitric oxide radical inhibition activity method, where Antibacterial Activity is done by Cup Plate Method. The MEBW (Methanolic extract of Buxus wallichiana ) showed an IC50 value of 29.2+2.10, where as ABBW (Aqueous extract of Buxus wallichiana ), CEBW(cholroform extract of Buxus wallichiana ) and PEBW (Petroleum ether extract of Buxus wallichiana ) produced IC50 values of 190+5.33, 647.5+16.43 and 747.5+10.2 respectively in DPPH method. When assayed for Antibacterial Activity MEBW at 500 pg/ml produced zone of inhibition of 26mm against Klebsiella aerogenes organism, against proteus vulgaris organism, CEBW, AEBW at 500 pg/ml produced zone of inhibition of 19mm.Against bacillus premiles, b. aerogenes and b.coacoagulans MEBW at 500 pg/ml shown zone of inhibition of 17, 19 and 24 mm respectively. The result of this study reveal that Buxus wallichiana Baill could be considered as potential source of natural anti oxidant and anti bacterial plant.
KEYWORDS: Anti oxidant, antimicrobial, in vitro, DPPH, Antibacterial.
INTRODUCTION:
The Buxus wallichiana Baill wood is commonly called as Himalayan Box Wood belongs to family Buxaceae. Box Wood is an evergreen monoecious shrub growing to an height of six meters with variable form. The green branches are initially pubescent, later glabrous, oleogreeen angular and densely covered with ovate leaves, which are usually opposite. Leaves are nearly sessile, opposite, nearly lanceolate to ovate 2-7 cms long. Flowers are clusters, auxiliary open in early spring. The fruit is capsule oblong, 5-6mm long seed. (Fleming 1990, Chopra et al 1992) Box Wood traditionally used as antihelmentic, analgesic, bitter tonic, anti rheumatic, purgative anti syphilitic, vermifuge and as hair growth stimulant.(Husain et al 1992, Chopra et al 1992). The plant contains large number of alkaloids, the main alkaloids have been isolated including buxemenol E, buxaltine H, n-3-bezoyl cyclohexobuxidine F, buxatine, buxepidine (Kvaltinova et al 1991, Husain et al 1992). Steroidal alkaloid buxemenol E was found to have hypotensive effect in rats attributed by central and peripheral...