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Birkhuser Verlag, Basel, 2007
Inflamm. res. 56 (2007) 981041023-3830/07/030098-7DOI 10.1007/s00011-006-6058-2 Inflammation Research
Expression analysis of the TAB2 protein in adult mouse tissues
C. Orelio and E. Dzierzak
Erasmus University Medical Centre, Dept. of Cell Biology and Genetics, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands,
Fax: ++31 10 408 9468, e-mail: [email protected]
Received 10 April 2006; returned for revision 1 September 2006; accepted by J. Di Battista 13 September 2006
Abstract. Background: The Interleukin-1 (IL-1) signaling component TAK1 binding protein 2 (TAB2) plays a role in activating the NFkB and JNK signaling pathways. Additionally, TAB2 functions in the nucleus as a repressor of NFkB-mediated gene regulation.
Objective: To obtain insight into the function of TAB2 in the adult mouse, we analyzed the in vivo TAB2 expression pattern.
Materials and methods: Cell lines and adult mouse tissues were analyzed for TAB2 protein expression and localization.
Results: Immunohistochemical staining for TAB2 protein revealed expression in the vascular endothelium of most tissues, hematopoietic cells and brain cells. While TAB2 is localized in both the nucleus and the cytoplasm in cell lines, cytoplasmic localization predominates in hematopoietic tissues in vivo.
Conclusions: The TAB2 expression pattern shows striking similarities with previously reported IL-1 receptor expression and NFkB activation patterns, suggesting that TAB2 in vivo is playing a role in these signaling pathways.
Key words: TAB2 Map3k7ip2 NF-kappaB Interleukin-1 Hematopoiesis
Introduction
The cytokine Interleukin-1 (IL-1) is a well-known regulator within the hematopoietic system. IL-1 can act on several different hematopoietic cell types and plays a role in regulating their proliferation and differentiation, depending on the target cell and the presence of additional cytokines [13]. IL-1 has also been shown to play a role in the migration and radio-protection of immature hematopoietic cells [4, 5], indicating
its versatile role within the hematopoietic system. Moreover, IL-1 plays an important role in inflammatory reactions [6]. IL-1 is a potent inducer of fever and acute phase response in the adult [3] and importantly, IL-1 has been implicated in several inflammatory-related diseases, such rheumatoid arthritis, the neurodegenerative Alzheimers disease and in vascular diseases such as atherosclerosis [79].
The regulation of these different biological processes by IL-1 is mediated via signalling from the IL-1RI towards downstream signalling pathways, including the NFkB pathway [6]. Signalling via NFkB members...