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Antonie van Leeuwenhoek (2014) 105:731741 DOI 10.1007/s10482-014-0129-0
ORIGINAL PAPER
Frederiksenia canicola gen. nov., sp. nov. isolated from dogs and human dog-bite wounds
Bo_
zena M. Korczak Magne Bisgaard
Henrik Christensen Peter Kuhnert
Received: 19 November 2013 / Accepted: 28 January 2014 / Published online: 8 February 2014 Springer International Publishing Switzerland 2014
Abstract Polyphasic analysis was done on 24 strains of Bisgaard taxon 16 from ve European countries and mainly isolated from dogs and human dog-bite wounds. The isolates represented a phenotypically and genetically homogenous group within the family Pasteurellaceae. Their phenotypic prole was similar to members of the genus Pasteurella. Matrix-assisted laser desorption/ionization time-of-ight mass spec-trometry clearly identied taxon 16 and separated it from all other genera of Pasteurellaceae showing a characteristic peak combination. Taxon 16 can be further separated and identied by a RecN protein signature sequence detectable by a specic PCR. In all phylogenetic analyses based on 16S rRNA, rpoB, infB and recN genes, taxon 16 formed a monophyletic
branch with intraspecies sequence similarity of at least99.1, 90.8, 96.8 and 97.2 %, respectively. Taxon 16 showed closest genetic relationship with Bibersteinia trehalosi as to the 16S rRNA gene (95.9 %), the rpoB(89.8 %) and the recN (74.4 %), and with Actinobacillus lignieresii for infB (84.9 %). Predicted genome similarity values based on the recN gene sequences between taxon 16 isolates and the type strains of known genera of Pasteurellaceae were below the genus level. Major whole cell fatty acids for the strain HPA 21T are C14:0, C16:0, C18:0 and C16:1 x7c/C15:0 iso
2OH. Major respiratory quinones are menaquinone-8, ubiquinone-8 and demethylmenaquinone-8. We propose to classify these organisms as a novel genus and species within the family of Pasteurellaceae named Frederiksenia canicola gen. nov., sp. nov. The type strain is HPA 21T (= CCUG 62410T = DSM 25797T).
Keywords Bisgaard taxon 16
Pasteurellaceae Taxonomy Phylogeny
Zoonosis
Introduction
Presently, the family Pasteurellaceae is one of the largest bacterial families, with many taxa still awaiting proper classication (Christensen and Bisgaard 2008; Gregersen et al. 2009; Kuhnert et al. 2010; Foster et al. 2011; Christensen et al. 2011; Hansen et al. 2012). Some members of the Pasteurellaceae are frequently
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