Global Diversity of Ascidiacea

Abstract

The class Ascidiacea presents fundamental opportunities for research in the fields of development, evolution, ecology, natural products and more. This review provides a comprehensive overview of the current knowledge regarding the global biodiversity of the class Ascidiacea, focusing in their taxonomy, main regions of biodiversity, and distribution patterns. Based on analysis of the literature and the species registered in the online World Register of Marine Species, we assembled a list of 2815 described species. The highest number of species and families is found in the order Aplousobranchia. Didemnidae and Styelidae families have the highest number of species with more than 500 within each group. Sixty percent of described species are colonial. Species richness is highest in tropical regions, where colonial species predominate. In higher latitudes solitary species gradually contribute more to the total species richness. We emphasize the strong association between species richness and sampling efforts, and discuss the risks of invasive species. Our inventory is certainly incomplete as the ascidian fauna in many areas around the world is relatively poorly known, and many new species continue to be discovered and described each year.

About the Authors:

Noa Shenkar

* E-mail: [email protected]

Affiliation: Department of Biology, University of Washington, Seattle, Washington, United States of America

Billie J. Swalla

Introduction

Ascidians (Phylum Chordata, Class Ascidiacea), or sea squirts, are the largest and most diverse class of the sub-phylum Tunicata (also known as Urochordata). They comprise approximately 3000 described species found in all marine habitats from shallow water to the deep sea [1]–[3]. The group was initially difficult for zoologists to classify systematically, although ascidians were recognized as a distinct group as early as Aristotle [1]. The first clear description of an ascidian was made by Schlosser in 1756 in a letter entitled “An account of a curious, fleshy, coral-like substance”. This specimen was dredged along the British Islands and was actually what we know now as the widely distributed colonial ascidian Botryllus schlosseri [4].

The name “tunicate” (sub-phylum Tunicata) was first coined by Lamarck [5] for ascidians, pyrosomes, and salps [6], [7]. The name originates from the polysaccharide-containing tunic that envelops the animal and forms a somewhat flexible skeleton [1]. Milne Edwards [8] mistakenly included the Bryozoa in this group, and both, together with the Brachiopoda, were included in the Mollusca by Hancock [9]. Savigny [10] also recognized the Tunicata (ascidians, salps, doliolids, and appendicularians) as a distinct group separate from the Mollusca [6]. Finally, the chordate nature of the ascidian tadpole larva was recognized by Kowalevsky [11], and they were reclassified with chordates [12]. The name Urochordata was not used until Balfour [13] created it as a replacement name for Tunicata, presumably to emphasize the chordate affinity. Indeed, recent phylogenomic studies place the tunicates as the sister group to the vertebrates [14]–[16], suggesting that they are our closest relatives among the invertebrates, which provides a fertile ground for evolutionary and developmental studies [17].

Following the original classification of Lahille [18], the class Ascidiacea is now divided into three orders based on the structure of the adult branchial sac: Aplousobranchia (simple), Phlebobranchia (vascular) and Stolidobranchia (folded) (Fig. 1). This is the current classification used by most ascidian taxonomists that also corresponds to molecular phylogenetic analysis based on the 18S rDNA [7], [19] as opposed to Perrier's [20] division that was based upon the position of the gonads and other morphological considerations and comprised only two orders: Enterogona and PleurogonaAscidians belonging to the order Aplousobranchia are all colonial while the Phlebobranchia and Stolidobranchia include both colonial and solitary species [7].

[Figure omitted. See PDF.]

Figure 1. Ascidian branchial sac structure, a distinguishing taxonomic character.

a) A simple branchial arrangement in an aplousobranch (Didemnum sp.). Arrows pointing out the straight stigmata rows. Photo: A. Shoob. Scale bar 1 mm; b) phlebobranch (Ascidia sp.) with longitudinal blood vessels; c) stolidobranch (Herdmania momus) with branchial folds. Photos: N Shenkar. Scale bar 1 mm and 4 mm respectively.

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Adult ascidians are sessile, inhabiting a wide variety of habitats such as soft sediments, coral reefs and rocky substrates. They successfully foul various artificial substrata such as jetties, ship hulls, floating docks and other man-made structures all over the world [21], [22]. They remain sessile following larval settlement throughout their adult life, so they cannot avoid salinity or temperature changes, and thus larval behavior is critical [23], [24]. Only a few species can survive in salinities below 20–25‰ [22], [25], or above 44‰ [26], (Shenkar N. unpublished results). The tropical Ecteinascidia thurstoni has been recorded along the Suez Canal in habitats with salinity reaching 46‰ overgrowing metal pilings of jetties [26], while several species inhabit marine lakes in Indonesia with salinity of 28.5‰ [27]. In salinities below 22‰ larval development is severely affected [24], as is the health of adult zooids [28]. Nonetheless, highly tolerant species such as Ciona intestinalis survive a wide range of salinities (12–40‰), and can withstand short periods of lower salinities (<11‰) [29], [30]. In general, ascidians exhibit a wider tolerance to temperature range than salinities [22], [31]. Antarctic species can tolerate temperature as low as -1.9°C [32], while others can survive seawater temperature higher than 35°C in the Arabian Gulf [33].

Both salinity and temperature are among the most important environmental variables influencing ascidian recruitment and reproduction [31], [34]–[36]. Other factors that may affect spatial distribution and recruitment include light, substrate type, hydrodynamics, predation and competition [22], [34], [37]. Understanding the role of these factors in ascidian recruitment, dispersal and survival is crucial to our understanding of ascidian global distribution patterns.

Ascidians are a key ecological group because of their invasive potential and ability to thrive in eutrophic (nutrient-rich) environments. Introductions of non-indigenous ascidians into harbors in both tropical and temperate waters are now commonplace, with the rate of introductions increasing, sometimes creating severe damage to natural fauna by overgrowth [1], [22], [38]–[41] (reviewed in a special issue of Aquatic Invasions January 2009). For example, the solitary ascidians Styela clava and Ciona intestinalis have had an adverse effect on aquaculture along Canada's east coast, mainly on mussel culture [42]–[44]. S. clava, when extremely abundant, may result in significantly decreased mussel growth and also cause severe problems in crop handling, resulting in increased production costs estimated at $4.5 million [45]. In contrast, several species of ascidians are cultured for food primarily in Japan, Korea and France. The solitary ascidian Halocynthia roretzi has long been a popular seafood in Japan and Korea, with a market value of $18 million in 2006 [46]. Recently, a unique infectious agent has been identified as the cause of mass mortality of these cultured ascidians [47].

Ascidians provide a fertile ground for studies in the field of natural products. Similar to sponges and bryozoans, many ascidians avoid predation or fouling by producing noxious secondary metabolites [48]–[52]. Because of these properties, numerous species of ascidians may thus be a potential source of new anti-cancer compounds [53], [54]. Trabectedin (earlier known as ecteinascidin-743, commercial name Yondelis®), a marine-derived alkaloid isolated from extracts of Ecteinascidia turbinate, is now being used in treatment of soft-tissue sarcomas [55], [56]. Antimalarial compounds have been isolated from the solitary ascidians Microcosmus helleri, Ascidia sydneiensis and Phallusia nigra [57], and numerous other compounds with anti-cancer, anti-viral and anti-bacterial capabilities are in various clinical trial stages by the pharmaceutical industry. The management and use of these organisms as sources of natural products is dependent, however, on understanding their taxonomy, the integrative basis of biology.

Ascidians have a poor fossil record [58]. Although calcareous spicules of distinctive shapes are found in some species of the families Polycitoridae, Pyuridae, and especially the Didemnidae [59], [60], their fossils are rarely reported by paleontologists [61]. This is possibly due to their susceptibility to dissolution, and small size; many are less than 0.1 mm [60]. Fossil didemnid ascidian spicules were encountered in rocks from various regions around the world, usually dating to the Late Pliocene-Early Pleistocene period [61], [62]. Eight specimens of a solitary fossil tunicate have been discovered with a body size of 2–4 cm; they resemble the extant Clavelina genus and are presumably ∼520 million years old [63].

Currently there are numerous web-based sources of taxonomic inventories (e.g., Encyclopedia of Life http://www.eol.org, Integrated Taxonomic Information System http://www.itis.gov), but only a few websites are dedicated to ascidians (e.g., The Dutch ascidians Home Page http://www.ascidians.com, Ascidian Home Page for United States http://depts.washington.edu/ascidian/), and they do not aim to provide an inventory list. Unfortunately, most web-based datasets often lack updates due to limitations in funding and expertise. The Ascidiacea World Database (http://www.marinespecies.org/ascidiacea/), which is a part of the World Register of Marine Species (WoRMS), is unique; it contains a comprehensive list of ascidian species, including information on synonymy, taxonomic literature, and distribution [64]. This database is the result of a joint effort of several ascidian taxonomists who constantly update and revise the information. With the aid of this database and the large taxonomic literature, our aim is to provide a systematic review of the class Ascidiacea, describe the main regions of highest biodiversity, discuss the risks of invasive species, and summarize the current trends in ascidian global distribution patterns.

Methods

Biogeographic distribution

Ascidian specimens are held by museums and similar institutions all over the world. However, only a few institutions provide reliable on-line options to search their collections (e.g., Smithsonian Invertebrate Zoology Collections, The Santa Barbara Museum of Natural History, Yale Peabody Museum Catalog Service, The Online Zoological Collections of Australian Museums). In these on-line collections we were able to find invaluable unpublished information regarding species distribution, and verify the occurrence of certain species in their native or introduced range. In addition, a literature search was done to record the number of species identified in various regions of the world in order to provide an estimate of global species richness. It is important to note that the numbers we provide represent the exact number of species mentioned in each citation.

Maps and geographic regions

Species distribution information was compiled based on the geographic regions of the Exclusive Economic Zone division v5 standard map provided by VLIZ Maritime Boundaries Geodatabase [65].

Species names and systematic validation

We followed the taxonomic classification and used the tabular keys of Monniot et al. 1991 [1] (revised by F. Monniot and G. Lambert 2008–2009, unpublished data). Annual check-lists are published on-line by the Catalogue of Life [66], and the Encyclopedia of Life [67]. Both databases are connected to the World Register of Marine Species (WoRMS) check list, to which the Ascidiacea World Database belongs [64]. Therefore, to avoid confusion, only the valid names and classification provided by the Ascidiacea World Database http://www.marinespecies.org/ascidiacea/ were used for systematic analysis of families, genera etc. Division of colonial versus solitary species was based on the Monniot et al. 1991 keys. Taxonomic contribution was analyzed according to the authority index in the World Ascidiacea Database, and only first authors were taken into consideration.

Records of non-indigenous ascidians

In order to compile a current list of non-indigenous ascidians, we gathered data not only from the available literature but also from different governmental reports which often provide the first record of an introduced species. In addition, valuable information was obtained from the proceedings of the International Invasive Sea Squirt conferences (J Exp Mar Biol Ecol 342 (1), 2007 and Aquat Inv 4 (1) 2009). The list includes only species that are mentioned as introduced or non-indigenous. Species that are classified as “cryptogenic” (species that cannot be reliably demonstrated as being either introduced or native, 68) were not included.

Results

Systematic division of ascidian species

Our systematic list includes 2815 valid species of ascidians. The highest number of species and families is found in the order Aplousobranchia, with approximately 50% of the species (1480) in the class Ascidiacea (Fig. 2a). Based on the classification of the Ascidiacea World Database, there are currently 26 families in the class Ascidiacea, of which 13 belong to the Aplousobranchia, with the Didemnidae having the highest number of species (578). The genera with the highest number of described species are Aplidium (259) and Didemnum (228), in the Aplousobranchia. However, the highest number of genera per family was found in the Styelidae (38), order Stolidobranchia, which also has the second highest number of species (535) (Fig. 2b). The majority of described species in the Ascidiacea are colonial (1730, 61.5%) (Table S1, supporting material).

[Figure omitted. See PDF.]

Figure 2. Systematic division of ascidian species.

a) Number and percentage of species per order; b) number of species by family within each order.

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Discovery rate and author contribution

The discovery rate of ascidian species from 1756 until 2010 is presented in Fig. 3a. The rate of discovery has accelerated since 1950, when the major taxonomists of this group, P. Kott, C. and F. Monniot, and R.H. Millar began publishing. Over 1600 species have been described by these experts including the numerous descriptions by C.P. Sluiter and W.A. Herdman from the late 19th century-beginning of the 20th century. Figure 3b summarizes the contribution of the major taxonomists to total ascidian species described. Only authors responsible for more than 100 descriptions are mentioned by name; Claude and Françoise Monniot were grouped together due to their numerous collaborative publications.

[Figure omitted. See PDF.]

Figure 3. Discovery rate and author contribution.

a) Cumulative number of valid ascidian species described between 1750–2010; b) Percentage and number of species described per taxonomic authority. Note: only taxonomic authorities with more than 100 species are mentioned by name.

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Non-indigenous ascidians

Review of the literature resulted in records of 64 non-indigenous species (Table 1). Thirty three species are colonial. Half of the introduced species (32) belong to the order Stolidobranchia, the rest divide between the other two orders. Almost half of the species (30) have records only from the northern hemisphere, 13 have records only from the southern hemisphere, and 21 have records from both sections.

[Figure omitted. See PDF.]

Table 1. Documented locations of non-indigenous ascidians.

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Records of introduction of ascidians in tropical waters are mainly from Hawaii, Guam and Panama. Of the 64 documented global non-indigenous species, 27 species have records in tropical regions. However, only 14 have records that are restricted to tropical environments (Ascidia archaia, Cnemidocarpa areolata, Cnemidocarpa irene, Didemnum cineraceum, Didemnum sp. (Hawaii), Distaplia stylifera, Herdmania pallida, Lissoclinum fragile, Polyandrocarpa anguinea, Polyandrocarpa sp., Polycarpa aurita, Polycarpa tumida, Polyclinum constellatum, Pyura vittata). The majority of species (50) have introduction records from temperate environments, including both the northern and southern hemispheres. There are no records yet of non-indigenous ascidians from the arctic.

Ascidian global distribution

Fig. 4 provides a summary of species richness in different regions of the world. A complete list of sites and references is provided in Table 2. The highest number of ascidian species is found in the Indo-Pacific, with inventory numbers such as 317 species from New-Caledonia, 187 species from the Western Pacific ocean, 117 and 102 species from Guam and Gulf of Siam area (numbers represent the exact number of species mentioned in each citation). The ascidian fauna along the coasts of Australia and New Zealand was studied extensively, resulting in records of 717 species from Australia, 249 species from Tasmania, and 124 species from New Zealand. At higher latitudes, the Mediterranean and Japan each represent areas with high number of species with about 229 species from the Mediterranean and 163 species in Japan. Antarctica and South Africa also have extensive records of ascidian species of 107 and 168 species respectively. The North American coasts have been studied thoroughly with approximately 170 species along both the Atlantic and Pacific coasts.

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Figure 4. Ascidian global distribution (abyssal species not included).

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[Figure omitted. See PDF.]

Table 2. Ascidian regional species richness.

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Discussion

Even though the class Ascidiacea has been the object of much scientific interest in the last decade [170], there are extensive regions around the world where very little collecting of ascidians has been done, resulting in very low number of described ascidian species and general lack of data (e.g., South and Central America, Canada, Alaska, and many thousands of islands in the tropical west Pacific). The current study reveals a strong association between species richness and sampling efforts. In addition, there is a clear trend of arrival and spread of non-indigenous species that put the endemic fauna at risk. Both of these issues emphasize the need for additional research in the field of ascidian biodiversity and biogeography.

In geographical areas where taxonomists have long been active, we typically found high numbers of species. The majority of the described ascidian species (more than 60%) are attributed to only seven taxonomic experts. This is demonstrated in the high species richness found in Australia [121], New Caledonia [122], Japan [128], the Caribbean Sea [171], and South Africa [172]–[174]. In contrast, along the coasts of South America, Indian Ocean, and Eastern Atlantic, there are vast areas with only scarce information regarding the occurrence of ascidians, and in some cases the only information comes from studies that may be out of date and not representative of the diversity these areas currently exhibit [154], [175]. For instance, the ascidian fauna of the Western Mediterranean has been studied in great detail and has been recorded in number of publications with an estimate of 165 described species [127]. The nearby Red Sea, which supports one of the most diverse ecosystems in the world [176] is represented by only 47 described species [152]. Thus, this discrepancy appears to be a result of less research and fewer sampling efforts, rather than a decrease in ascidian diversity [177]. Our inventory of 2815 described species of ascidians is certainly incomplete, with an estimation that approximately 3000 species remain to be discovered and described (Appeltans et al. 2011 unpublished data). Applying molecular approaches may further assist in locating cryptic speciation of a single species.

The high diversity of some of the ascidian families is remarkable. With approximately 26 families in the class Ascidiacea, the colonial Didemnidae family contains 20% of the described species, possibly due to highly diverse Didemnum genera, with more than 200 species. The Styelidae family is also highly diverse with 38 genera, and 535 described species, colonial and solitary. Colonial species characterize more than 60% of the described species. The high diversity of colonial ascidians is increasingly important since many contain very active secondary metabolites important to the pharmaceutical industry [178].

In general, it has been shown that in tropical environments colonial species dominate the substrate [179]. This is attributed to their asexual reproduction and indeterminate growth which provide them with a significant advantage for the exploitation of tropical habitats. Thus there are many more colonial ascidian species than solitary species in the tropics, representing about 80% of the species [125], [126], [131], [143], [180]. Although colonial ascidians are generally considered a minor benthic component on exposed surfaces of the natural coral reefs they can rapidly overgrow corals and outcompete them for space during periods of nutrient enrichment [181]–[183]. Since ascidians are able to filter even minute particulate matter [184], [185], any rise in nutrient levels and organic material in coastal waters will have a direct influence on their abundance.

In temperate waters solitary ascidians comprise 52% of the American fauna [154], and 75% in European waters (but this includes abyssal forms, almost all of which are solitary), [125], [186]. In the Antarctic, 58% of the species are solitary [32]. It is possible that solitary ascidians in the Antarctic and the deep sea, many of which are stalked, have an advantage over encrusting colonial species since most of the benthos is composed of soft sediments, so their vertical growth lifts them above the sediment. This three dimensional structure may improve food capture and assimilation during periods of winter inactivity and sedimentation [187]. In addition, since in solitary ascidians fertilization and larval development usually occur in the water column (in contrast to colonial species which are brooders), it is possible that they have a higher potential for dispersal [74]. This may also be advantageous in the Antarctic in cases of anchor ice formation [188], and ice scouring [189] which have a key role in determining marine biodiversity in high latitudes, emphasizing the importance of larval dispersal processes.

Historical baselines for comparison to present day from museum collections and published literature are required in order to understand and respond to changes in global biodiversity [190]. The current study provides a list of 64 non-indigenous ascidians (NIAs) with published records of introduction. This number is likely to be an underestimate, due to difficulty in taxonomic identification of aplousobranch species in particular. In some cases it may be difficult to determine if a certain species record is of a new introduction, or of a previously undiscovered natural population [113]. Lambert [40] suggests two criteria for the designation of NIAs in Guam, following the general guidelines of Chapman and Carlton [68] for determining non-indigenous species: (1) restricted to artificial surfaces and (2) an extra Indo-West Pacific distribution. The first criterion may be especially important especially in tropical environments which may be more resistant to invasion due to their diverse communities [191], [192]. In temperate and cold water environments there are records of rapid spread of NIAs on natural substrates such as Didemnum vexillum (Gulf of Maine) [109], [193] and Microcosmus squamiger (Western Mediterranean) [112]. A molecular approach, therefore, may be more relevant in revealing the status of a certain species [194]–[196].

The majority of records of NIAs are from cold water environments, suggesting this environment may be more favorable to introductions of ascidians. Nonetheless, nearly half of the NIAs have geographical records from tropical environments. Under lab conditions, at high temperature, the growth rate of NIAs was higher compared to that of native species [197], and they were able to tolerate significantly higher temperatures [198]. Thus, there is growing evidence that global warming may facilitate a shift northward by non-native species, accelerating homogenization of the global biota [199]. Nevertheless, high rates of endemism can be found in tropical environments such as the Great Barrier Reef [121], New Caledonia and French Polynesia [122], [131], and also in unique environments such as Southern New Zealand [123], and the Antarctic, with its isolated and homogeneous fauna [32].

The class Ascidiacea presents vast opportunities for research in the fields of evolution and development, physiology, natural products, and marine bioinvasion. Yet, there are many areas around the world that are relatively poorly known, and in others the available data should be updated and revised. Many more species are yet to be discovered, contributing to our accumulating knowledge of this unique group.

Supporting Information

[Figure omitted. See PDF.]

Table S1.

Systematic division of ascidian species following the Ascidiacea World Database [67].

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(DOC)

Acknowledgments

We express our gratitude to G. Lambert for her advice and helpful suggestions and to C. Primo for her collaboration. N. Shenkar would like to thank the Israeli Taxonomy Initiative and the Dan-David foundation for financial support.

Author Contributions

Conceived and designed the experiments: NS BJS. Performed the experiments: NS. Analyzed the data: NS. Contributed reagents/materials/analysis tools: BJS. Wrote the paper: NS BJS.

Citation: Shenkar N, Swalla BJ (2011) Global Diversity of Ascidiacea. PLoS ONE6(6): e20657. https://doi.org/10.1371/journal.pone.0020657

References

1. Monniot C, Monniot F, Laboute P (1991) Coral Reef Ascidians of New Caledonia. Paris: ORSTOM. C. MonniotF. MonniotP. Laboute1991Coral Reef Ascidians of New Caledonia.ParisORSTOM

2. Cameron CB, Garey JR, Swalla BJ (2000) Evolution of the chordate body plan: new insights from phylogenetic analyses of deuterostome phyla. Proc Natl Acad Sci USA 97: 4469–4474.CB CameronJR GareyBJ Swalla2000Evolution of the chordate body plan: new insights from phylogenetic analyses of deuterostome phyla.Proc Natl Acad Sci USA9744694474

3. Kott P (2005) New and little-known species of Didemnidae (Ascidiacea, Tunicata) from Australia (Part 3). J Nat Hist 39: 2409–2497.P. Kott2005New and little-known species of Didemnidae (Ascidiacea, Tunicata) from Australia (Part 3).J Nat Hist3924092497

4. Forbes E, Hanley SCT (1853) A history of British Mollusca and their shells. 4 vols. London: John Van Voorst. E. ForbesSCT Hanley1853A history of British Mollusca and their shells.4 vols. LondonJohn Van Voorst

5. Lamarck JB (1816) Histoire naturelle des animaux sans vertèbres. Tome III. Tuniciers. Paris: Déterville. pp. 80–130C.JB Lamarck1816Histoire naturelle des animaux sans vertèbres. Tome III. Tuniciers.ParisDéterville80130C

6. Lambert CC (2005) Historical introduction, overview, and reproductive biology of the protochordates. Can J Zool 83: 1–7.CC Lambert2005Historical introduction, overview, and reproductive biology of the protochordates.Can J Zool8317

7. Zeng L, Swalla BJ (2005) Molecular phylogeny of the protochordates: chordate evolution. Can J Zool 83: 24–33.L. ZengBJ Swalla2005Molecular phylogeny of the protochordates: chordate evolution.Can J Zool832433

8. Milne Edwards H (1843) Éléments de zoologie. Vol. 2. Animaux sans vertèbres 313–316.H. Milne Edwards1843Éléments de zoologie. Vol. 2.Animaux sans vertèbres313316

9. Hancock A (1850) On the anatomy of the fresh water Bryozoa. Ann Nat Hist 2: 173–204.A. Hancock1850On the anatomy of the fresh water Bryozoa.Ann Nat Hist2173204

10. Savigny JC (1816) Mémoires sur les Animoux sans Vertèbres. Part 2. Paris: G Dufour. JC Savigny1816Mémoires sur les Animoux sans Vertèbres.Part 2. ParisG Dufour

11. Kowalevsky AO (1866) Entwicklungsgeschichte der einfachen Ascidien. Mem Acad Sci St Petersb 10: 1–19.AO Kowalevsky1866Entwicklungsgeschichte der einfachen Ascidien.Mem Acad Sci St Petersb10119

12. Raff RA, Love AC (2004) Kowalevsky, comparative evolutionary embryology, and the intellectual lineage of evo-devo. J Exp Zool (Mol Dev Evol) 302B: 19–34.RA RaffAC Love2004Kowalevsky, comparative evolutionary embryology, and the intellectual lineage of evo-devo.J Exp Zool (Mol Dev Evol)302B1934

13. Balfour FM (1881) A treatise of comparative embryology. London: Vol. 2.Macmillan. FM Balfour1881A treatise of comparative embryology.LondonVol. 2.Macmillan

14. Bourlat SJ, Juliusdottir T, Lowe CJ, Freeman R, Aronowicz J, et al. (2006) Deuterostome phylogeny reveals monophyletic chordates and the new phylum Xenoturbellida. Nature 444: 85–88.SJ BourlatT. JuliusdottirCJ LoweR. FreemanJ. Aronowicz2006Deuterostome phylogeny reveals monophyletic chordates and the new phylum Xenoturbellida.Nature4448588

15. Delsuc F, Brinkmann H, Chourrout D, Philippe H (2006) Tunicates and not cephalochordates are the closest living relatives of vertebrates. Nature 439: 965–968.F. DelsucH. BrinkmannD. ChourroutH. Philippe2006Tunicates and not cephalochordates are the closest living relatives of vertebrates.Nature439965968

16. Swalla BJ, Smith AB (2008) Deciphering deuterostome phylogeny: molecular, morphological and palaeontological perspectives. Phil Trans R Soc B 363: 1557–1568.BJ SwallaAB Smith2008Deciphering deuterostome phylogeny: molecular, morphological and palaeontological perspectives.Phil Trans R Soc B36315571568

17. Nishida H, Sawada K (2001) Macho-1 encodes a localized mRNA in ascidian eggs that specifies muscle fate during embryogenesis. Nature 409: 724–729.H. NishidaK. Sawada2001Macho-1 encodes a localized mRNA in ascidian eggs that specifies muscle fate during embryogenesis.Nature409724729

18. Lahille F (1886) Sur la classification des Tuniciers. CR Acad Sci Paris 102: 1573–1575.F. Lahille1886Sur la classification des Tuniciers.CR Acad Sci Paris10215731575

19. Tsagkogeorga G, Turon X, Hopcroft RR, Tilak M, Feldstein T, et al. (2009) An updated 18S rRNA phylogeny of tunicates based on mixture and secondary structure models. BMC Evol Biol 9: 187.G. TsagkogeorgaX. TuronRR HopcroftM. TilakT. Feldstein2009An updated 18S rRNA phylogeny of tunicates based on mixture and secondary structure models.BMC Evol Biol9187

20. Perrier JO (1898) Note sur la classification des Tuniciers. CR Acad Sci Paris 126: 1758–1762.JO Perrier1898Note sur la classification des Tuniciers.CR Acad Sci Paris12617581762

21. Lambert G (2001) A global overview of ascidian introductions and their possible impact on the endemic fauna. In: Sawada H, Yokosawa H and Lambert CC (ed.), The Biology of Ascidians. Tokyo, Springer-Verlag, 249–257.G. Lambert2001A global overview of ascidian introductions and their possible impact on the endemic fauna. In: Sawada H, Yokosawa H and Lambert CC (ed.), The Biology of Ascidians.Tokyo, Springer-Verlag,249257

22. Lambert G (2005) Ecology and natural history of the protochordates. Can J Zool 83: 34–50.G. Lambert2005Ecology and natural history of the protochordates.Can J Zool833450

23. Svane I, Young CM (1989) The ecology and behavior of ascidian larvae. Oceanogr Mar Biol Ann Rev 27: 45–90.I. SvaneCM Young1989The ecology and behavior of ascidian larvae.Oceanogr Mar Biol Ann Rev274590

24. Vazquez E, Young CM (1996) Responses of compound ascidian larvae to haloclines. Mar Ecol Prog Ser 133: 179–190.E. VazquezCM Young1996Responses of compound ascidian larvae to haloclines.Mar Ecol Prog Ser133179190

25. Sims LL (1984) Osmoregulatory capabilities of three macrosympatric stolidobranch ascidians, Styela clava Herdman, S. plicata (Lesueur) and S. montereyensis (Dall). J Exp Mar Biol Ecol 82: 117–129.LL Sims1984Osmoregulatory capabilities of three macrosympatric stolidobranch ascidians, Styela clava Herdman, S. plicata (Lesueur) and S. montereyensis (Dall).J Exp Mar Biol Ecol82117129

26. Gab Alla AAFA (2008) Distribution of the sea squirt Ecteinascidia thurstoni Herdman, 1890 (Ascidiacea: Perophoridae) along Suez Canal and Egyptian Red Sea Coasts. Oceanologia 50: 239–253.AAFA Gab Alla2008Distribution of the sea squirt Ecteinascidia thurstoni Herdman, 1890 (Ascidiacea: Perophoridae) along Suez Canal and Egyptian Red Sea Coasts.Oceanologia50239253

27. Monniot F (2009) Some ascidians from Indonesian marine lakes (Raja Ampat Islands, West Papua). Zootaxa 2106: 13–40.F. Monniot2009Some ascidians from Indonesian marine lakes (Raja Ampat Islands, West Papua).Zootaxa21061340

28. Dijkstra J, Dutton A, Westerman E, Harris L (2008) Heart rate reflects osmotic stress levels in two introduced colonial ascidians Botryllus schlosseri and Botrylloides violaceus. Mar Biol 154: 805–811.J. DijkstraA. DuttonE. WestermanL. Harris2008Heart rate reflects osmotic stress levels in two introduced colonial ascidians Botryllus schlosseri and Botrylloides violaceus.Mar Biol154805811

29. Dybern BI (1967) Settlement of sessile animals on eternite slabs in two polls near Bergen. Sarsia 29: 137–180.BI Dybern1967Settlement of sessile animals on eternite slabs in two polls near Bergen.Sarsia29137180

30. Therriault TW, Herborg LM (2008) Predicting the potential distribution of the vase tunicate Ciona intestinalis in Canadian waters: informing a risk assessment. ICES J Mar Sci 65: 788–794.TW TherriaultLM Herborg2008Predicting the potential distribution of the vase tunicate Ciona intestinalis in Canadian waters: informing a risk assessment.ICES J Mar Sci65788794

31. Goodbody I (2004) Diversity and distribution of ascidians (Tunicata) at Twin Cays, Belize. Atoll Res Bull 524: 1–20.I. Goodbody2004Diversity and distribution of ascidians (Tunicata) at Twin Cays, Belize.Atoll Res Bull524120

32. Primo C, Vazquez E (2009) Antarctic ascidians: an isolated and homogeneous fauna. Polar Res 28: 403–414.C. PrimoE. Vazquez2009Antarctic ascidians: an isolated and homogeneous fauna.Polar Res28403414

33. Monniot C, Monniot F (1997) Records of ascidians from Bahrain, Arabian Gulf with three new species. J Nat Hist 31: 1623–1643.C. MonniotF. Monniot1997Records of ascidians from Bahrain, Arabian Gulf with three new species.J Nat Hist3116231643

34. Millar RH (1971) The biology of ascidians. Adv Mar Biol 9: 1–100.RH Millar1971The biology of ascidians.Adv Mar Biol91100

35. Auker LA, Oviatt CA (2008) Factors influencing the recruitment and abundance of Didemnum in Narragansett Bay, RhodeIsland. ICES J Mar Sci 65: 765–769.LA AukerCA Oviatt2008Factors influencing the recruitment and abundance of Didemnum in Narragansett Bay, RhodeIsland.ICES J Mar Sci65765769

36. Shenkar N, Loya Y (2008) The solitary ascidian Herdmania momus: native (Red Sea) vs. non-indigenous (Mediterranean) populations. Biol Inv 10: 1431–1439.N. ShenkarY. Loya2008The solitary ascidian Herdmania momus: native (Red Sea) vs. non-indigenous (Mediterranean) populations.Biol Inv1014311439

37. Shenkar N, Zeldman Y, Loya Y (2008) Ascidian recruitment patterns on an artificial reef in Eilat (Red Sea). Biofouling 24: 119–128.N. ShenkarY. ZeldmanY. Loya2008Ascidian recruitment patterns on an artificial reef in Eilat (Red Sea).Biofouling24119128

38. Lambert CC, Lambert G (1998) Non-indigenous ascidians in southern California harbours and marinas. Mar Biol 130: 675–68.CC LambertG. Lambert1998Non-indigenous ascidians in southern California harbours and marinas.Mar Biol13067568

39. Coles SL, DeFelice RC, Eldredge LG, Carlton JT (1999) Historical and recent introductions of non-indigenous marine species into Pearl Harbor, Oahu, Hawaiian Islands. Mar Biol 135: 147–158.SL ColesRC DeFeliceLG EldredgeJT Carlton1999Historical and recent introductions of non-indigenous marine species into Pearl Harbor, Oahu, Hawaiian Islands.Mar Biol135147158

40. Lambert G (2002) Nonindigenous ascidians in tropical waters. Pac Sci 56: 291–298.G. Lambert2002Nonindigenous ascidians in tropical waters.Pac Sci56291298

41. Cohen AN, Harris LH, Bingham BL, Carlton JT, Chapman JW, et al. (2005) Rapid assessment survey for exotic organisms in southern California bays and harbors, and abundance in port and non-port areas. Biol Inv 7: 995–1002.AN CohenLH HarrisBL BinghamJT CarltonJW Chapman2005Rapid assessment survey for exotic organisms in southern California bays and harbors, and abundance in port and non-port areas.Biol Inv79951002

42. Thompson R, MacNair N (2004) An overview of the clubbed tunicate (Styela clava) in Prince Edward Island. PEI Department of Agriculture, Fisheries, Aquaculture and Forestry Technical Report 234, VIII +. 29 p.R. ThompsonN. MacNair2004An overview of the clubbed tunicate (Styela clava) in Prince Edward Island.PEI Department of Agriculture, Fisheries, Aquaculture and Forestry Technical Report 234, VIII +29

43. Bourque D, Davidson J, MacNair NG, Arsenault G, LeBlanc AR, et al. (2007) Reproduction and early life history of an invasive ascidian Styela clava Herdman in Prince Edward Island, Canada. J Exp Mar Biol Ecol 342: 78–84.D. BourqueJ. DavidsonNG MacNairG. ArsenaultAR LeBlanc2007Reproduction and early life history of an invasive ascidian Styela clava Herdman in Prince Edward Island, Canada.J Exp Mar Biol Ecol3427884

44. Howes S, Herbinger CM, Darnell P, Vercaemer B (2007) Spatial and temporal patterns of recruitment of the tunicate Ciona intestinalis on a mussel farm in Nova Scotia, Canada. J Exp Mar Biol Ecol 342(1): 85–92.S. HowesCM HerbingerP. DarnellB. Vercaemer2007Spatial and temporal patterns of recruitment of the tunicate Ciona intestinalis on a mussel farm in Nova Scotia, Canada.J Exp Mar Biol Ecol34218592

45. Raynolds C, Fortune R (2003) What is the view from the boat? Atlantic Canadian Tunicate Workshop Proceedings, Atlantic Veterinary College, PEI Canada March 2003 23.C. RaynoldsR. Fortune2003What is the view from the boat?Atlantic Canadian Tunicate Workshop Proceedings, Atlantic Veterinary College, PEI Canada March 200323

46. Nguyen TTT, Taniguchi N, Nakajima M, Na-Nakorn U, Sukumasavin N, et al. (2007) Aquaculture of sea-pineapple, Halocynthia roretzi in Japan. Aquac Asia Mag 12: 21–23.TTT NguyenN. TaniguchiM. NakajimaU. Na-NakornN. Sukumasavin2007Aquaculture of sea-pineapple, Halocynthia roretzi in Japan.Aquac Asia Mag122123

47. Kumagai A, Suto A, Ito H, Tanabe T, Takahashi K, et al. (2010) Mass mortality of cultured ascidians Halocynthia roretzi associated with softening of the tunic and flagellate-like cells. Dis Aquat Organ 90: 223–2343.A. KumagaiA. SutoH. ItoT. TanabeK. Takahashi2010Mass mortality of cultured ascidians Halocynthia roretzi associated with softening of the tunic and flagellate-like cells.Dis Aquat Organ902232343

48. Teo SLM, Ryland JS (1994) Toxicity and palatability of some British ascidians. Mar Biol 120: 297–303.SLM TeoJS Ryland1994Toxicity and palatability of some British ascidians.Mar Biol120297303

49. Teo SLM, Ryland JS (1995) Potential antifouling mechanisms using toxic chemicals in some British ascidians. J Exp Mar Biol Ecol 188: 49–62.SLM TeoJS Ryland1995Potential antifouling mechanisms using toxic chemicals in some British ascidians.J Exp Mar Biol Ecol1884962

50. Davis AR (1998) Antifouling defense in a subtidal guild of temperate zone encrusting invertebrates. Biofouling 12: 305–320.AR Davis1998Antifouling defense in a subtidal guild of temperate zone encrusting invertebrates.Biofouling12305320

51. Pisut DP, Pawlik JR (2002) Anti-predatory chemical defenses of ascidians: secondary metabolites or inorganic acids? J Exp Mar Biol Ecol 270: 203–214.DP PisutJR Pawlik2002Anti-predatory chemical defenses of ascidians: secondary metabolites or inorganic acids?J Exp Mar Biol Ecol270203214

52. McClintock JB, Amsler MO, Amsler CD, Southworth KJ, Petrie C, et al. (2004) Biochemical composition, energy content and chemical antifeedant and antifoulant defenses of the colonial Antarctic ascidian Distaplia cylindrica. Mar Biol 145: 885–894.JB McClintockMO AmslerCD AmslerKJ SouthworthC. Petrie2004Biochemical composition, energy content and chemical antifeedant and antifoulant defenses of the colonial Antarctic ascidian Distaplia cylindrica.Mar Biol145885894

53. Wright AE, Forleo DA, Gunawardana GP, Gunasekera SP, Koehn FE, et al. (1990) Antitumor tetrahydroisoquinoline alkaloids from the colonial ascidian Ecteinascidia turbinata. J Org Chem 55: 4508–4512.AE WrightDA ForleoGP GunawardanaSP GunasekeraFE Koehn1990Antitumor tetrahydroisoquinoline alkaloids from the colonial ascidian Ecteinascidia turbinata.J Org Chem5545084512

54. Scotto KW (2002) ET-743: more than an innovative mechanism of action. Anti-Cancer Drugs 13: suppl. 1S3–6.KW Scotto2002ET-743: more than an innovative mechanism of action.Anti-Cancer Drugs13suppl. 1S36

55. Casali PG, Sanfilippo R, D'Incalci M (2010) Trabectedin therapy for sarcomas. Curr Opin Oncol 22: 342–346.PG CasaliR. SanfilippoM. D'Incalci2010Trabectedin therapy for sarcomas.Curr Opin Oncol22342346

56. Martinez-Trufero J, Alfaro J, Felipo F, Alvarez M, Madani J, et al. (2010) Response to trabectedin treatment in a highly pretreated patient with an advanced meningeal hemangiopericytoma. Anti-Cancer Drug 21: 795–79.J. Martinez-TruferoJ. AlfaroF. FelipoM. AlvarezJ. Madani2010Response to trabectedin treatment in a highly pretreated patient with an advanced meningeal hemangiopericytoma.Anti-Cancer Drug2179579

57. Mendiola J, Hernández H, Sariego I, Rojas L, Otero A, et al. (2006) Antimalarial activity from three ascidians: an exploration of different marine invertebrate phyla. Trans Roy Soc Trop Med H 100: 909–916.J. MendiolaH. HernándezI. SariegoL. RojasA. Otero2006Antimalarial activity from three ascidians: an exploration of different marine invertebrate phyla.Trans Roy Soc Trop Med H100909916

58. Brookfield ME (1988) Where are all the fossil sea squirts? Micropaleontology 34: 277–283.ME Brookfield1988Where are all the fossil sea squirts?Micropaleontology34277283

59. Lambert G, Lambert CC, Lowenstam HA (1990) Protochordate biomineralization. In Skeletal Biomineralization: Patterns, Processes and Evolutionary Trends. In: Carter JG, editor. NY: Van Nostrand Reinhold. pp. 461–469.G. LambertCC LambertHA Lowenstam1990Protochordate biomineralization. In Skeletal Biomineralization: Patterns, Processes and Evolutionary Trends.JG CarterNYVan Nostrand Reinhold461469

60. Kott P (2001) The Australian Ascidiacea. Part 4: Aplousobranchia (3), Didemnidae. Mem Queensland Mus 47(1): 1–407.P. Kott2001The Australian Ascidiacea. Part 4: Aplousobranchia (3), Didemnidae.Mem Queensland Mus4711407

61. Varol O (1996) A review and classification of fossil didemnid ascidian spicules. J Micropalaeontol 15: 135–149.O. Varol1996A review and classification of fossil didemnid ascidian spicules.J Micropalaeontol15135149

62. Sagular EK (2009) Fossil didemnid ascidian spicule records in the Plio-Quaternary marine clastics of the Antalya Basin (Eastern Mediterranean) and their stratigraphic calibration to new nannofossil data. Geosci J 13: 121–131.EK Sagular2009Fossil didemnid ascidian spicule records in the Plio-Quaternary marine clastics of the Antalya Basin (Eastern Mediterranean) and their stratigraphic calibration to new nannofossil data.Geosci J13121131

63. Chen JY, Huang DY, Peng QQ, Chi HM, Wang XQ, et al. (2003) The first tunicate from the Early Cambrian of South China. P Natl Acad Sci USA 100: 8314–8318.JY ChenDY HuangQQ PengHM ChiXQ Wang2003The first tunicate from the Early Cambrian of South China.P Natl Acad Sci USA10083148318

64. Shenkar N, Gittenberger A, Lambert G, Rius M, Moreira Da Rocha R, et al. (2010) World Ascidiacea Database.N. ShenkarA. GittenbergerG. LambertM. RiusR. Moreira Da Rocha2010World Ascidiacea Database.http://www.marinespecies.org/ascidiacea. Consulted on 10-28-2010. http://www.marinespecies.org/ascidiacea. Consulted on 10-28-2010.

65. VLIZ (2010) VLIZ2010Maritime Boundaries Geodatabase, version X. http://www.vliz.be/vmdcdata/marbound. Consulted on 11-03-2010. Maritime Boundaries Geodatabase, version X. http://www.vliz.be/vmdcdata/marbound. Consulted on 11-03-2010.

66. Bisby FA, Roskov YR, Orrell TM, Nicolson D, Paglinawan LE, et al. (2010) FA BisbyYR RoskovTM OrrellD. NicolsonLE Paglinawan2010Species 2000 & ITIS Catalogue of Life, 2nd October 2010. Digital resource at http://www.catalogueoflife.org/col. Species 2000: Reading, UK. Species 2000 & ITIS Catalogue of Life, 2nd October 2010. Digital resource at http://www.catalogueoflife.org/col. Species 2000: Reading, UK.

67. Wilson EO (2003) The encyclopedia of life. Trends Ecol Evol 18: 77–80.EO Wilson2003The encyclopedia of life.Trends Ecol Evol187780

68. Chapman JW, Carlton JT (1991) A test of criteria for introduced species: the global invasion by the isopod Synidotea laevidorsalis (Miers, (1881). J Crustacean Biol 11: 386–400.JW ChapmanJT Carlton1991A test of criteria for introduced species: the global invasion by the isopod Synidotea laevidorsalis (Miers, (1881).J Crustacean Biol11386400

69. Gittenberger A (2009) Invasive tunicates on Zeeland and Prince Edward Island mussels, and management practices in The Netherlandss. Aquat Inv 4: 279–281.A. Gittenberger2009Invasive tunicates on Zeeland and Prince Edward Island mussels, and management practices in The Netherlandss.Aquat Inv4279281

70. Coutts ADM, Dodgshun TJ (2007) The nature and extent of organisms in vessel sea-chests: A protected mechanism for marine bioinvasions. Mar Pollut Bull 54: 875–886.ADM CouttsTJ Dodgshun2007The nature and extent of organisms in vessel sea-chests: A protected mechanism for marine bioinvasions.Mar Pollut Bull54875886

71. Rocha RM, Kremer LP, Baptista MS, Metri R (2009) Bivalve cultures provide habitat for exotic tunicates in southern Brazil. Aquat Inv 4: 195–205.RM RochaLP KremerMS BaptistaR. Metri2009Bivalve cultures provide habitat for exotic tunicates in southern Brazil.Aquat Inv4195205

72. Rocha RM (2010) Monitoring ascidians on natural and anthropogenic habitats in Bocas del Toro, Panama. III IISSC Woods Hole, April. RM Rocha2010Monitoring ascidians on natural and anthropogenic habitats in Bocas del Toro, Panama.III IISSC Woods Hole, April

73. Izquierdo-Muñoz A, Diaz-Valdés M, Ramos-Esplá AA (2009) Recent non-indigenous ascidians in the Mediterranean Sea. Aquat Inv 4: 59–64.A. Izquierdo-MuñozM. Diaz-ValdésAA Ramos-Esplá2009Recent non-indigenous ascidians in the Mediterranean Sea.Aquat Inv45964

74. Shenkar N, Loya Y (2009) Non-indigenous ascidians (Chordata: Tunicata) along the Mediterranean coast of Israel. Marine Biodiversity Records 2: 166.N. ShenkarY. Loya2009Non-indigenous ascidians (Chordata: Tunicata) along the Mediterranean coast of Israel.Marine Biodiversity Records2166

75. Zenetos A, Gofas S, Verlaque M, Inar ME, Garci'a Raso JE, et al. (2010) Alien species in the Mediterranean Sea by 2010. A contribution to the application of European Union's Marine Strategy Framework Directive (MSFD). Part I Spatial distribution. Medit Mar Sci 11: 381–493.A. ZenetosS. GofasM. VerlaqueME InarJE Garci'a Raso2010Alien species in the Mediterranean Sea by 2010. A contribution to the application of European Union's Marine Strategy Framework Directive (MSFD). Part I Spatial distribution.Medit Mar Sci11381493

76. Lambert CC, Lambert G (2003) Persistence and differential distribution of nonindigenous ascidians in harbors of the Southern California Bight. Mar Ecol Prog Ser 259: 145–161.CC LambertG. Lambert2003Persistence and differential distribution of nonindigenous ascidians in harbors of the Southern California Bight.Mar Ecol Prog Ser259145161

77. Marins FO, Novaes RLM, Rocha RM, Junqueira AOR (2010) Non indigenous ascidians in port and natural environments in a tropical Brazilian bay. Zoologia 27: 213–221.FO MarinsRLM NovaesRM RochaAOR Junqueira2010Non indigenous ascidians in port and natural environments in a tropical Brazilian bay.Zoologia27213221

78. Jaffar Ali HA, Sivakumar V, Tamilselvi M (2009) Distribution of Alien and Cryptogenic Ascidians along the Southern Coasts of Indian Peninsula. World J Fish Mar Sci 1: 305–312.HA Jaffar AliV. SivakumarM. Tamilselvi2009Distribution of Alien and Cryptogenic Ascidians along the Southern Coasts of Indian Peninsula.World J Fish Mar Sci1305312

79. Rocha RM, Kremer LP (2005) Introduced ascidians in Paranaguá Bay, Paraná, Southern Brazil. Revista Brasileira de Zoologia 22: 1170–1184.RM RochaLP Kremer2005Introduced ascidians in Paranaguá Bay, Paraná, Southern Brazil.Revista Brasileira de Zoologia2211701184

80. Oliveira FRR, Lotufo TMC (2010) New records of introduced ascidians at Ceara State harbors, Northern Brazil. III IISSC Woods Hole, April. FRR OliveiraTMC Lotufo2010New records of introduced ascidians at Ceara State harbors, Northern Brazil.III IISSC Woods Hole, April

81. DeFelice RC, Eldredge LG, Carlton JT (2001) Nonindigenous marine invertebrates. In: A Guidebook of Introduced Marine Species in Hawaii , Eldredge LG, Smith CM, editors. Honolulu: Bishop Museum. RC DeFeliceLG EldredgeJT Carlton2001Nonindigenous marine invertebrates. A Guidebook of Introduced Marine Species in HawaiiLG EldredgeCM SmithHonoluluBishop Museum

82. Mead A, Carlton JT, Griffiths CL, Rius M (2011) Introduced and cryptogenic marine and estuarine species of South Africa. J Nat Hist In Press. A. MeadJT CarltonCL GriffithsM. Rius2011Introduced and cryptogenic marine and estuarine species of South Africa.J Nat Hist In Press

83. Carman MR, Grunden DW (2010) First occurrence of the invasive tunicate Didemnum vexillum in eelgrass habitat. Aquat Inv 5: 23–29.MR CarmanDW Grunden2010First occurrence of the invasive tunicate Didemnum vexillum in eelgrass habitat.Aquat Inv52329

84. Brewin BI (1946) Ascidians in the vicinity of the Portobello Marine Biological Station, Otago Harbour. T Roy Soc NZ 76: 87–131.BI Brewin1946Ascidians in the vicinity of the Portobello Marine Biological Station, Otago Harbour.T Roy Soc NZ7687131

85. Wiltshire K, Rowling K, Deveney M (2010) Introduced marine species in South Australia: a review of records and distribution mapping. South Australian Research and Development Institute (Aquatic Sciences), Adelaide. SARDI Publication No. F2010/000305-1. SARDI Research Report Series No. 468. 232 p.K. WiltshireK. RowlingM. Deveney2010Introduced marine species in South Australia: a review of records and distribution mapping.South Australian Research and Development Institute (Aquatic Sciences), Adelaide. SARDI Publication No. F2010/000305-1. SARDI Research Report Series No. 468232

86. Hewitt CL, Campbell ML, Thresher RE, Martin RB, Boyd S, et al. (2004) Introduced and cryptogenic species in Port Phillip Bay, Victoria, Australia. Mar Biol 144: 183–202.CL HewittML CampbellRE ThresherRB MartinS. Boyd2004Introduced and cryptogenic species in Port Phillip Bay, Victoria, Australia.Mar Biol144183202

87. Tatian M, Schwindt E, Lagger C, Varela MM (2010) Colonization of Patagonian harbours (SW Atlantic) by an invasive sea squirt (Chordata, Ascidiacea). Spixiana 33: 111–117.M. TatianE. SchwindtC. LaggerMM Varela2010Colonization of Patagonian harbours (SW Atlantic) by an invasive sea squirt (Chordata, Ascidiacea).Spixiana33111117

88. Castilla JC, Neill PE (2009) Marine bioinvasions in the Southern Pacific: status, ecology, economic impacts, conservation and management. In Biological Invasions in Marine Ecosystems: Ecological, Management, and Geographic Perspectives. In: Rilov G, Crooks J, editors. Berlin Springer-Verlag Heidelberg. pp. 439–458.JC CastillaPE Neill2009Marine bioinvasions in the Southern Pacific: status, ecology, economic impacts, conservation and management. In Biological Invasions in Marine Ecosystems: Ecological, Management, and Geographic Perspectives.G. RilovJ. CrooksBerlin Springer-Verlag Heidelberg439458

89. Callahan AG, Deibel D, McKenzie CH, Hall JR, Rise ML (2010) Survey of harbours in Newfoundland for indigenous and non-indigenous ascidians and an analysis of their cytochrome c oxidase I gene sequences. Aquat Inv 5: 31–39.AG CallahanD. DeibelCH McKenzieJR HallML Rise2010Survey of harbours in Newfoundland for indigenous and non-indigenous ascidians and an analysis of their cytochrome c oxidase I gene sequences.Aquat Inv53139

90. Cohen AN (2005) AN Cohen2005Guide to Exotic Species of San Francisco Bay. San Francisco Estuary Institute. Oakland, California. www.exoticsguide.org. Guide to Exotic Species of San Francisco Bay. San Francisco Estuary Institute. Oakland, California. www.exoticsguide.org.

91. Arenas F, Bishop JDD, Carlton JT, Dyrynda PJ, Farnham WF, et al. (2006) Alien species and other notable records from a rapid assessment survey of marinas on the south coast of England. J Mar Biol Assoc UK 86: 1329–1337.F. ArenasJDD BishopJT CarltonPJ DyryndaWF Farnham2006Alien species and other notable records from a rapid assessment survey of marinas on the south coast of England.J Mar Biol Assoc UK8613291337

92. Carver CE, Mallet AL, Vercaemer B (2006) Biological synopsis of the colonial tunicates, Botryllus schlosseri and Botrylloides violaceus. Canadian Manuscript Report of Fisheries and Aquatic Sciences. 2747 p.CE CarverAL MalletB. Vercaemer2006Biological synopsis of the colonial tunicates, Botryllus schlosseri and Botrylloides violaceus.Canadian Manuscript Report of Fisheries and Aquatic Sciences2747

93. Lejeusne C, Bock DG, Therriault TW, MacIsaac HJ, Cristescu ME (2010) Comparative phylogeography of two colonial ascidians reveals contrasting invasion histories in North America. Biol Inv 13: 635–650.C. LejeusneDG BockTW TherriaultHJ MacIsaacME Cristescu2010Comparative phylogeography of two colonial ascidians reveals contrasting invasion histories in North America.Biol Inv13635650

94. Lambert G (2003) New records of ascidians from the NE Pacific: a new species of Trididemnum, range extension and redescription of Aplidiopsis pannosum (Ritter, 1899) including its larva, and several nonindigenous species. Zoosystema 25: 665–679.G. Lambert2003New records of ascidians from the NE Pacific: a new species of Trididemnum, range extension and redescription of Aplidiopsis pannosum (Ritter, 1899) including its larva, and several nonindigenous species.Zoosystema25665679

95. Gollasch S, Haydar D, Minchin D, Wolff WJ, Reise K (2009) Introduced aquatic species of the North Sea coasts and adjacent brackish waters. In: Biological Invasions in Marine Ecosystems: Ecological, Management, and Geographic Perspectives , Rilov G, Crooks J, editors. Berlin Springer-Verlag Heidelberg. pp. 507–528.S. GollaschD. HaydarD. MinchinWJ WolffK. Reise2009Introduced aquatic species of the North Sea coasts and adjacent brackish waters. Biological Invasions in Marine Ecosystems: Ecological, Management, and Geographic PerspectivesG. Rilov J. CrooksBerlin Springer-Verlag Heidelberg507528

96. Stoner DS, Ben-Shlomo R, Rinkevich B, Weisman IL (2002) Genetic Variability of Botryllus schlosseri invasions to the East and West Coasts of USA. Mar Ecol Prog Ser 243: 93–100.DS StonerR. Ben-ShlomoB. RinkevichIL Weisman2002Genetic Variability of Botryllus schlosseri invasions to the East and West Coasts of USA.Mar Ecol Prog Ser24393100

97. Brewin BI (1950) Ascidians of New Zealand. Part IV. Ascidians in the vicinity of Christchurch. T Roy Soc NZ 78: 344–353.BI Brewin1950Ascidians of New Zealand. Part IV. Ascidians in the vicinity of Christchurch.T Roy Soc NZ78344353

98. Lambert G (2007) Washington state 2006 survey for invasive tunicates with records from previous surveys. Updated October 2010. Prepared for Washington Dept. of Fish and Wildlife. G. Lambert2007Washington state 2006 survey for invasive tunicates with records from previous surveys.Updated October 2010. Prepared for Washington Dept. of Fish and Wildlife

99. Seo KS, Yoon L (2009) A first assessment of invasive marine species on Chinese and Korean Coasts. In: Biological Invasions in Marine Ecosystems: Ecological, Management, and Geographic Perspectives , Rilov G, Crooks J, editors. Berlin Springer-Verlag Heidelberg. pp. 577–586.KS SeoL. Yoon2009A first assessment of invasive marine species on Chinese and Korean Coasts. Biological Invasions in Marine Ecosystems: Ecological, Management, and Geographic PerspectivesG. RilovJ. CrooksBerlin Springer-Verlag Heidelberg577586

100. Smith KF, Cahill PL, Fidler AE (2010) First record of the solitary ascidian Ciona savignyi Herdman, 1882 in the Southern Hemisphere. Aquat Inv 5: 363–368.KF SmithPL CahillAE Fidler2010First record of the solitary ascidian Ciona savignyi Herdman, 1882 in the Southern Hemisphere.Aquat Inv5363368

101. Zvyagintsev D, Sanamyan ÊE, Kashenko SD (2007) On the Introduction of the Ascidian Ciona savignyi Herdman, 1882 into Peter the Great Bay, Sea of Japan. Russ J Mar Biol 33: 133–136.D. ZvyagintsevÊE SanamyanSD Kashenko2007On the Introduction of the Ascidian Ciona savignyi Herdman, 1882 into Peter the Great Bay, Sea of Japan.Russ J Mar Biol33133136

102. Reinhardt JF, Stefaniak LM, Hudson DM, Mangiafico J, Gladych R, et al. (2010) First record of the non-native light bulb tunicate Clavelina lepadiformis (Müller, 1776) in the northwest Atlantic. Aquat Inv 5: 185–190.JF ReinhardtLM StefaniakDM HudsonJ. MangiaficoR. Gladych2010First record of the non-native light bulb tunicate Clavelina lepadiformis (Müller, 1776) in the northwest Atlantic.Aquat Inv5185190

103. Godwin S, Harris L, Charette A, Moffitt R (2008) The marine invertebrate species associated with the biofouling of derelict fishing gear in the Papahanoumokuakea–Marine National Monument. A focus on marine non-native species transport. Kaneohe, HI: Hawaii Institute for Marine Biology. 26 p.S. GodwinL. HarrisA. CharetteR. Moffitt2008The marine invertebrate species associated with the biofouling of derelict fishing gear in the Papahanoumokuakea–Marine National Monument. A focus on marine non-native species transport.Kaneohe, HIHawaii Institute for Marine Biology26

104. Lambert G (2004) The south temperate and Antarctic ascidian Corella eumyota reported in two harbours in northwestern France. J Mar Biol Assoc UK 84: 239–241.G. Lambert2004The south temperate and Antarctic ascidian Corella eumyota reported in two harbours in northwestern France.J Mar Biol Assoc UK84239241

105. Nagar AE, Huys R, Bishop JDD (2010) Widespread occurrence of the southern hemisphere ascidian Corella eumyota Traustedt, 1882 on the Atlantic coast of Iberia. Aquat Inv 5: 169–173.AE NagarR. HuysJDD Bishop2010Widespread occurrence of the southern hemisphere ascidian Corella eumyota Traustedt, 1882 on the Atlantic coast of Iberia.Aquat Inv5169173

106. Monniot F (1983) Ascidies littorales de Guadeloupe I. Didemnidae. Bull Mus Natl Hist Nat, Paris 4e ser, 5: 5–49.F. Monniot1983Ascidies littorales de Guadeloupe I. Didemnidae. Bull Mus Natl Hist Nat, Paris 4e ser,5549

107. Cole L, Lambert G (2009) Tunicata (Urochordata) of the Gulf of Mexico. Chapt. 73, 1209-1216. In: Felder D.L., Camp D.K., editors. Gulf of Mexico: Origin, Waters, and Biota. Vol. 1, Biodiversity. Texas A&M Univ. Press. L. ColeG. Lambert2009Tunicata (Urochordata) of the Gulf of Mexico. Chapt. 73, 1209-1216D.L. FelderD.K. CampGulf of Mexico: Origin, Waters, and Biota. Vol. 1, BiodiversityTexas A&M Univ. Pressin:. in:.

108. Griffith K, Mowat S, Holt RHF, Ramsay K, Bishop JDD, et al. (2009) First records in Great Britain of the invasive colonial ascidian Didemnum vexillum Kott, 2002. Aquat Inv 4: 581–590.K. GriffithS. MowatRHF HoltK. RamsayJDD Bishop2009First records in Great Britain of the invasive colonial ascidian Didemnum vexillum Kott, 2002.Aquat Inv4581590

109. Lambert G (2009) Adventures of a sea squirt sleuth: unraveling the identity of Didemnum vexillum, a global ascidian invader. Aquat Inv 4: 5–28.G. Lambert2009Adventures of a sea squirt sleuth: unraveling the identity of Didemnum vexillum, a global ascidian invader.Aquat Inv4528

110. Mastrototaro F, Brunetti R (2006) The non-indigenous ascidian Distaplia bermudensis in the Mediterranean: comparison with the native species Distaplia magnilarva and Distaplia lucillae sp nov. J Mar Biol Assoc UK 86: 181–185.F. MastrototaroR. Brunetti2006The non-indigenous ascidian Distaplia bermudensis in the Mediterranean: comparison with the native species Distaplia magnilarva and Distaplia lucillae sp nov.J Mar Biol Assoc UK86181185

111. Page MJ, Morrisey DJ, Handley SJ (2010) Biology, ecology and trials of potential methods for control of the introduced ascidian Eudistoma elongatum in Northland, New Zealand. III IISSC Woods Hole, April. MJ PageDJ MorriseySJ Handley2010Biology, ecology and trials of potential methods for control of the introduced ascidian Eudistoma elongatum in Northland, New Zealand.III IISSC Woods Hole, April

112. Turon X, Nishikawa T, Rius M (2007) Spread of Microcosmus squamiger (Ascidiacea: Pyuridae) in the Mediterranean Sea and adjacent waters. J Exp Mar Biol Ecol 342: 185–188.X. TuronT. NishikawaM. Rius2007Spread of Microcosmus squamiger (Ascidiacea: Pyuridae) in the Mediterranean Sea and adjacent waters.J Exp Mar Biol Ecol342185188

113. Lambert G, Shenkar N, Swalla BJS (2010) First Pacific record of the north Atlantic ascidian Molgula citrina- bioinvasion or circumpolar distribution? Aquatic Inv 5: 369–378.G. LambertN. ShenkarBJS Swalla2010First Pacific record of the north Atlantic ascidian Molgula citrina- bioinvasion or circumpolar distribution?Aquatic Inv5369378

114. Lambert G (2007) The nonindigenous ascidian Molgula ficus in California. Cah Biol Marine 48: 95–102.G. Lambert2007The nonindigenous ascidian Molgula ficus in California.Cah Biol Marine4895102

115. Haydar D (2010) What is natural? The scale and consequences of marine bioinvasions in the North Atlantic Ocean. Van Denderen BV, Groningen, The Netherlandss. 184 p.D. Haydar2010What is natural?The scale and consequences of marine bioinvasions in the North Atlantic Ocean. Van Denderen BV, Groningen, The Netherlandss184

116. Pérez-Portela R, Bishop JDD, Turon X (2010) A recent invader, Perophora japonica, in Europe: Temporal genetic change and spatial distribution of genetic diversity in the English Channel. III IISSC Woods Hole, April. R. Pérez-PortelaJDD BishopX. Turon2010A recent invader, Perophora japonica, in Europe: Temporal genetic change and spatial distribution of genetic diversity in the English Channel.III IISSC Woods Hole, April

117. Lambert G (2005) First North American record of the ascidian Perophora japonica. J Mar Biol Assoc UK 85: 1011–1012.G. Lambert2005First North American record of the ascidian Perophora japonica.J Mar Biol Assoc UK8510111012

118. Tovar-Hernández M, Suárez-Morales E, Yáñez-Rivera B (2010) The parasitic copepod Haplostomides hawaiiensis (Cyclopoida) from the invasive ascidian Polyclinum constellatum in the southern Gulf of California. Bull Mar Sci 86: 637–648.M. Tovar-HernándezE. Suárez-MoralesB. Yáñez-Rivera2010The parasitic copepod Haplostomides hawaiiensis (Cyclopoida) from the invasive ascidian Polyclinum constellatum in the southern Gulf of California.Bull Mar Sci86637648

119. Davis MH, Davis ME (2009) Styela clava (Tunicata, Ascidiacea) - a new threat to the Mediterranean shellfish industry? Aquat Inv 4: 283–289.MH DavisME Davis2009Styela clava (Tunicata, Ascidiacea) - a new threat to the Mediterranean shellfish industry?Aquat Inv4283289

120. Pineda MC, López-Legentil S, Turon X (2010) Global phylogeography of the solitary ascidian Styela plicata. III IISSC Woods Hole, April. MC PinedaS. López-LegentilX. Turon2010Global phylogeography of the solitary ascidian Styela plicata.III IISSC Woods Hole, April

121. Kott P (2006) Observations on non-didemnid ascidians in Australian waters. J Nat Hist 40: 169–234.P. Kott2006Observations on non-didemnid ascidians in Australian waters.J Nat Hist40169234

122. Monniot F (2007) Some comments on ascidians of New Caledonia. In: Payri CE, Richer de Forges B, editors. Compendium of marine species of New Caledonia. 117. Doc Sci Tech. pp. 349–356.F. Monniot2007Some comments on ascidians of New Caledonia.CE PayriB. Richer de ForgesCompendium of marine species of New Caledonia117Doc Sci Tech349356

123. Primo C, Vazquez E (2008) Zoogeography of the southern New Zealand, Tasmanian and southern African ascidian fauna. New Zeal J Mar Fresh 42: 233–256.C. PrimoE. Vazquez2008Zoogeography of the southern New Zealand, Tasmanian and southern African ascidian fauna.New Zeal J Mar Fresh42233256

124. Coll M, Piroddi C, Steenbeek J, Kaschner K, Ben Rais Lasram F, et al. (2010) The biodiversity of the Mediterranean Sea: estimates, patterns, and threats. PLoS One 5(8): e11842.M. CollC. PiroddiJ. SteenbeekK. KaschnerF. Ben Rais Lasram2010The biodiversity of the Mediterranean Sea: estimates, patterns, and threats.PLoS One58e11842

125. Monniot F, Monniot C (2001) Ascidians from the tropical western Pacific. Zoosystema 23: 201–383.F. MonniotC. Monniot2001Ascidians from the tropical western Pacific.Zoosystema23201383

126. Primo C, Vazquez E (2004) Zoogeography of the southern African ascidian fauna. J Biogeogr 31: 1987–2009.C. PrimoE. Vazquez2004Zoogeography of the southern African ascidian fauna.J Biogeogr3119872009

127. Koukouras A, Voultsiadou-Koukoura E, Kevrekidis T, Vafidis D (1995) Ascidian fauna of the Aegean sea with a check list of the Eastern Mediterranean and Black Sea species. Annales de l'Institut Oceanographique 71: 19–34.A. KoukourasE. Voultsiadou-KoukouraT. KevrekidisD. Vafidis1995Ascidian fauna of the Aegean sea with a check list of the Eastern Mediterranean and Black Sea species.Annales de l'Institut Oceanographique711934

128. Nishikawa T (1990) The ascidians of the Japan Sea. I. Publications of the Seto Marine Biological Laboratory 34: 73–148.T. Nishikawa1990The ascidians of the Japan Sea.I. Publications of the Seto Marine Biological Laboratory3473148

129. Lambert G (2003) Marine biodiversity of Guam: the Ascidiacea. Micronesica 35-36: 588–597.G. Lambert2003Marine biodiversity of Guam: the Ascidiacea.Micronesica35-36588597

130. Millar RH (1975) Ascidians from the Indo-West-Pacific region in the Zoologlcal Museum, Copenhagen (Tunicata, Ascidiacea). Steenstrupia 20: 205–336.RH Millar1975Ascidians from the Indo-West-Pacific region in the Zoologlcal Museum, Copenhagen (Tunicata, Ascidiacea).Steenstrupia20205336

131. Monniot C, Monniot F (1987) Les ascidies de Polynesie francaise. Memoires du Museum national d'Histoire naturelle, Paris (A) 136: 1–155.C. MonniotF. Monniot1987Les ascidies de Polynesie francaise.Memoires du Museum national d'Histoire naturelle, Paris (A)1361155

132. Abdul JAH, Sivakumar V (2007) Occurrence and distribution of ascidians in Vizhinjam Bay (south west coast of India). J Exp Mar Biol Ecol 342: 189–190.JAH AbdulV. Sivakumar2007Occurrence and distribution of ascidians in Vizhinjam Bay (south west coast of India).J Exp Mar Biol Ecol342189190

133. Primo C, Vazquez E (2007) Zoogeography of the Antarctic ascidian fauna in relation to the sub-Antarctic and South America. Antarct Sci 19: 321–336.C. PrimoE. Vazquez2007Zoogeography of the Antarctic ascidian fauna in relation to the sub-Antarctic and South America.Antarct Sci19321336

134. Sanamyan K (1993a) Ascidians from the NW Pacific region. 1. Polycitoridae. Ophelia 37: 163–173.K. Sanamyan1993aAscidians from the NW Pacific region. 1. Polycitoridae.Ophelia37163173

135. Sanamyan K (1993b) Ascidians from the NW Pacific region. 2. Molgulidae. Ophelia 38: 127–135.K. Sanamyan1993bAscidians from the NW Pacific region. 2. Molgulidae.Ophelia38127135

136. Sanamyan K (1996) Ascidians from the NW Pacific region. 3. Pyuridae. Ophelia 45: 199–209.K. Sanamyan1996Ascidians from the NW Pacific region. 3. Pyuridae.Ophelia45199209

137. Sanamyan K (1998) Ascidians from the NW Pacific region. 4. Polyclinidae and Placentelidae. Ophelia 48: 103–135.K. Sanamyan1998Ascidians from the NW Pacific region. 4. Polyclinidae and Placentelidae.Ophelia48103135

138. Sanamyan K (1998) Ascidians from the NW Pacific region. 5. Phlebobranchia. Ophelia, 49: 97–116.K. Sanamyan1998Ascidians from the NW Pacific region. 5. Phlebobranchia.Ophelia,4997116

139. Sanamyan K (1999) Ascidians from the NW Pacific region. 6. Didemnidae. Ophelia 51: 143–161.K. Sanamyan1999Ascidians from the NW Pacific region. 6. Didemnidae.Ophelia51143161

140. Sanamyan K (2000) Ascidians from the North-Western Pacific region. 7. Styelidae. Ophelia 53: 67–78.K. Sanamyan2000Ascidians from the North-Western Pacific region. 7. Styelidae.Ophelia536778

141. Ramos Espla AA, Buencuerpo V, Vasquez E, Lafargue F (1992) Some biogeographical remarks about the ascidian littoral fauna of the Straits of Gibraltar (Iberian sector). Bull de l'Inst Océan Monaco 9: 125–132.AA Ramos EsplaV. BuencuerpoE. VasquezF. Lafargue1992Some biogeographical remarks about the ascidian littoral fauna of the Straits of Gibraltar (Iberian sector).Bull de l'Inst Océan Monaco9125132

142. Vazquez E, Ramos-Espla A (1993) Eudistoma roseum n.sp (Ascidiacea, Polycitoridae) from the Iberian Atlantic coast. Ophelia 37: 95–100.E. VazquezA. Ramos-Espla1993Eudistoma roseum n.sp (Ascidiacea, Polycitoridae) from the Iberian Atlantic coast.Ophelia3795100

143. Kott P (1981) The ascidians of the reef flats of Fiji. Proc Linn Soc NSW 105: 147–212.P. Kott1981The ascidians of the reef flats of Fiji.Proc Linn Soc NSW105147212

144. Lambert CC, Lambert G, Kozloff EN (1987) Phylum Urochordata, Class Ascidiacea. In: Kozloff EN, editor. 23. U of Wash Press. pp. 467–479.CC LambertG. LambertEN Kozloff1987Phylum Urochordata, Class Ascidiacea. EN Kozloff23U of Wash Press467479In: Marine Invertebrates of the Pacific Northwest.

145. Rocha RM Faria SB, Moreno TR (2005) Ascidians from Bocas del Toro, Panama. I. Biodiversity. Caribb J Sci 41: 600–612.SB Rocha RM FariaTR Moreno2005Ascidians from Bocas del Toro, Panama. I. Biodiversity.Caribb J Sci41600612

146. Berrill NJ (1950) The Tunicata with an Account of the British Species. London: The Ray Soc.. 354 p.NJ Berrill1950The Tunicata with an Account of the British Species.LondonThe Ray Soc.354

147. Kott P (2004) Ascidiacea (Tunicata) in Australian waters of the Timor and Arafura Seas. Beagle Rec Mus Art Galleries NT 20: 37–81.P. Kott2004Ascidiacea (Tunicata) in Australian waters of the Timor and Arafura Seas.Beagle Rec Mus Art Galleries NT203781

148. Rodrigues SA, Rocha RM, Lotufo TMC (1998) Guia Ilustrado para Identificação das Ascídias do Estado de São Paulo. FAPESP, São Paulo. SA RodriguesRM RochaTMC Lotufo1998Guia Ilustrado para Identificação das Ascídias do Estado de São Paulo.FAPESP, São Paulo

149. Monniot C, Monniot F (1994) Additions to the inventory of eastern tropical Atlantic ascidians; arrival of cosmopolitan species. Bull Mar Sci 54: 71–93.C. MonniotF. Monniot1994Additions to the inventory of eastern tropical Atlantic ascidians; arrival of cosmopolitan species.Bull Mar Sci547193

150. Lee MR, Castilla JC, Fernandez M, Clarke M, Gonzalez C, Hermosilla C, Prado L, Rozbaczylo N, Valdovinos C (2008) Free-living benthic marine invertebrates in Chile. Revista Chliena De Historia Natural 81: 51–67.MR LeeJC CastillaM. FernandezM. ClarkeC. GonzalezC. HermosillaL. PradoN. RozbaczyloC. Valdovinos2008Free-living benthic marine invertebrates in Chile.Revista Chliena De Historia Natural815167

151. Millar RH (1966) Marine invertebrates of Scandinavia. 1. Tunicata. Ascidiacea. Scandinavian University books, Oslo. RH Millar1966Marine invertebrates of Scandinavia.1. Tunicata. Ascidiacea. Scandinavian University books, Oslo

152. Monniot C (2002) Stolidobranch ascidians from the tropical western Indian Ocean. Zool J Linn Soc 135: 65–120.C. Monniot2002Stolidobranch ascidians from the tropical western Indian Ocean.Zool J Linn Soc13565120

153. Abbott , DP , Newberry AT, Morris KM (1997) Section 6B: Ascidians (Urochordata). Reef and Shore Fauna of Hawaii. Bishop Museum Special Publ 64(6B).AbbottDPAT NewberryKM Morris1997Section 6B: Ascidians (Urochordata).Reef and Shore Fauna of Hawaii. Bishop Museum Special Publ646B

154. Van Name WG (1945) The North and South American ascidians. Bull Am Mus Nat Hist 84: 1–476.WG Van Name1945The North and South American ascidians.Bull Am Mus Nat Hist841476

155. Goodbody I (2003) The Ascidian fauna of Port Royal, Jamaica In Harbour and mangrove dwelling species. Bull Mar Sci 73: 457–476.I. Goodbody2003The Ascidian fauna of Port Royal, Jamaica In Harbour and mangrove dwelling species.Bull Mar Sci73457476

156. Hernández-Zanuy AC, Carballo JL (2001) Distribution and abundance of ascidian assemblages in Caribbean reef zones of the Golfo de Batabanó (Cuba). Coral Reefs 20: 159–162.AC Hernández-ZanuyJL Carballo2001Distribution and abundance of ascidian assemblages in Caribbean reef zones of the Golfo de Batabanó (Cuba).Coral Reefs20159162

157. Brunel P, Bossé L, Lamarche G (1998) Catalogue of the Marine Invertebrates of the Estuary and Gulf of Saint Lawrence. Canadian Special Publication of Fisheries and Aquatic Sciences 126: 405.P. BrunelL. BosséG. Lamarche1998Catalogue of the Marine Invertebrates of the Estuary and Gulf of Saint Lawrence.Canadian Special Publication of Fisheries and Aquatic Sciences126405

158. Kott P, Goodbody I (1982) Ascidians of Hong Kong. In: The Marine Flora and Fauna of Hong Kong and Southern China. Hong Kong: Hong Kong University Press. pp. 503–554.P. KottI. Goodbody1982Ascidians of Hong Kong. In: The Marine Flora and Fauna of Hong Kong and Southern China.Hong KongHong Kong University Press503554

159. Berrill NJ (1932) Ascidians of the Bermudas. Biological Bulletin 62: 77–88.NJ Berrill1932Ascidians of the Bermudas.Biological Bulletin627788

160. Goodbody I (1984) Ascidians from Caribbean shallow water localities. Studies on the Fauna of Curaçao and Other Caribbean Islands 67: 62–76.I. Goodbody1984Ascidians from Caribbean shallow water localities.Studies on the Fauna of Curaçao and Other Caribbean Islands676276

161. Monniot F, Monniot C (1997) Ascidians collected in Tanzania. J East Afr Nat Hist 86: 1–35.F. MonniotC. Monniot1997Ascidians collected in Tanzania.J East Afr Nat Hist86135

162. Millar RH (1956) Ascidians from Mozambique, East Africa. Ann Mag Nat Hist Nat Sci 9: 913–932.RH Millar1956Ascidians from Mozambique, East Africa.Ann Mag Nat Hist Nat Sci9913932

163. Millar RH (1988) Ascidians collected during the International Indian Ocean Expedition. J Nat Hist 22: 823–848.RH Millar1988Ascidians collected during the International Indian Ocean Expedition.J Nat Hist22823848

164. Rocha RM, Guerra-Castro E, Lira C, Paul SM, Hernández I, et al. (2010) Inventory of ascidians (Tunicata, Ascidiacea) from the National Park La Restinga, Isla Margarita, Venezuela. Biota Neotrop 10: 210–218.RM RochaE. Guerra-CastroC. LiraSM PaulI. Hernández2010Inventory of ascidians (Tunicata, Ascidiacea) from the National Park La Restinga, Isla Margarita, Venezuela.Biota Neotrop10210218

165. Pérès JM (1962) Sur une collection d'ascidies de la cote israelienne de la mer rouge et de la peninsula du Sinai. Bull Sea Fish Res Haifa 30: 39–47.JM Pérès1962Sur une collection d'ascidies de la cote israelienne de la mer rouge et de la peninsula du Sinai.Bull Sea Fish Res Haifa303947

166. Monniot C (1973) Redescription de six ascidies du golfe d'Elat récoltées par H. Schumacher. Israel Journal of Zoology 22: 51–62.C. Monniot1973Redescription de six ascidies du golfe d'Elat récoltées par H. Schumacher.Israel Journal of Zoology225162

167. Monniot C (1974) Ascidies littorales et bathyales recoltees au cours de la campagne Biacores: Phlebobranches et Stolidobranches. Bull Mus Natn Hist Nat Paris 3 173(251): 1327–1352.C. Monniot1974Ascidies littorales et bathyales recoltees au cours de la campagne Biacores: Phlebobranches et Stolidobranches.Bull Mus Natn Hist Nat Paris317325113271352

168. Iturralde M (1996) Taxonomy, abundance, and vertical distribution of the ascidians from Tagus Cove, Isabela Island. Charles Darwin Research Station Annual Report 1988-1989: 120–123.M. Iturralde1996Taxonomy, abundance, and vertical distribution of the ascidians from Tagus Cove, Isabela Island.Charles Darwin Research Station Annual Report1988-1989120123

169. Monniot C, Monniot F (1997) Records of ascidians from Bahrain, Arabian Gulf, with three new species. J Nat Hist 31: 1623–1643.C. MonniotF. Monniot1997Records of ascidians from Bahrain, Arabian Gulf, with three new species.J Nat Hist3116231643

170. Pourquié , O (2001) A macho way to make muscles. Nature 409: 679–680.PourquiéO2001A macho way to make muscles.Nature409679680

171. Goodbody I (2006) Caribbean Sea Squirts: The Goodbody collection. The Mona Institute of Applied Sciences, Jamaica. I. Goodbody2006Caribbean Sea Squirts: The Goodbody collection.The Mona Institute of Applied Sciences, Jamaica

172. Millar RH (1955) A collection of ascidians from South Africa. P Zool Soc Lond 125: 169–221.RH Millar1955A collection of ascidians from South Africa.P Zool Soc Lond125169221

173. Millar RH (1962) Further descriptions of South African ascidians. Annals of the South African Museum 46: 113–221.RH Millar1962Further descriptions of South African ascidians.Annals of the South African Museum46113221

174. Millar RH (1964) South African ascidians collected by Th. Mortensen, with some additional material. Videnskabelige Meddelelser fra Dansk Naturhistorisk Forening, bd. 127: 159–180.RH Millar1964South African ascidians collected by Th. Mortensen, with some additional material.Videnskabelige Meddelelser fra Dansk Naturhistorisk Forening, bd.127159180

175. Millar RH (1965) Ascidians from the tropical coast of west Africa. Atlantide Rep 8: 247–255.RH Millar1965Ascidians from the tropical coast of west Africa.Atlantide Rep8247255

176. Loya Y (1991) Changes in a Red Sea Coral Community Structure: A Long-Term Case History Study. In: Woodwell , editor. The earth in transition Patterns and Processes of Biotic Impoverishment. Woods Hole Oceanographic Institution, Massachusetts. Y. Loya1991Changes in a Red Sea Coral Community Structure: A Long-Term Case History Study.WoodwellThe earth in transition Patterns and Processes of Biotic ImpoverishmentWoods Hole Oceanographic Institution, Massachusetts

177. Shenkar N, Loya Y (2008) Ecology and systematics of the ascidian fauna in the Gulf of Eilat (Aqaba). In “Aqaba-Eilat, the Improbable Gulf. Environment, Biodiversity and Preservation” Editor FD Por, Magnes, Jerusalem197–208.N. ShenkarY. Loya2008Ecology and systematics of the ascidian fauna in the Gulf of Eilat (Aqaba). In “Aqaba-Eilat, the Improbable Gulf. Environment, Biodiversity and Preservation” Editor FD Por, Magnes, Jerusalem197208

178. Menna M (2009) Antitumor potential of natural products from Mediterranean ascidians. Phytochem Rev 8: 461–472.M. Menna2009Antitumor potential of natural products from Mediterranean ascidians.Phytochem Rev8461472

179. Jackson JBC (1977) Competition on marine and hard substrata: the adaptive significance of solitary and colonial strategies. Am Nat 11: 743–767.JBC Jackson1977Competition on marine and hard substrata: the adaptive significance of solitary and colonial strategies.Am Nat11743767

180. Monniot C, Monniot F (1985) Ascidies littorals de Guadeloupe. IX: Caractéristiques des populations, écologie, rapports avec la faune mondiale. Tethys 11: 203–213.C. MonniotF. Monniot1985Ascidies littorals de Guadeloupe. IX: Caractéristiques des populations, écologie, rapports avec la faune mondiale.Tethys11203213

181. Bak RPM, Lambrechts DYM, Joenje M, Nieuwland G, Van Veghel MLJ (1996) Long-term changes on coral reefs in booming populations of a competitive colonial ascidian. Mar Ecol Prog Ser 133: 303–306.RPM BakDYM LambrechtsM. JoenjeG. NieuwlandMLJ Van Veghel1996Long-term changes on coral reefs in booming populations of a competitive colonial ascidian.Mar Ecol Prog Ser133303306

182. Shenkar N, Bronstein O, Loya Y (2008) Population dynamics of a coral reef ascidian in a deteriorating environment. Mar Ecol Prog Ser 367: 163–171.N. ShenkarO. BronsteinY. Loya2008Population dynamics of a coral reef ascidian in a deteriorating environment.Mar Ecol Prog Ser367163171

183. Vargas-Ángel B, Godwin LS, Asher J, Brainard R (2009) Invasive didemnid tunicate spreading across coral reefs at remote Swains Island, American Sāmoa. Coral Reefs 28: 53.B. Vargas-ÁngelLS GodwinJ. AsherR. Brainard2009Invasive didemnid tunicate spreading across coral reefs at remote Swains Island, American Sāmoa.Coral Reefs2853

184. Bak RPM, Joenje M, de Jong I, Lambrechts DYM, Nieuwland G (1998) Bacterial suspension feeding by coral reef benthic organisms. Mar Ecol Prog Ser 175: 285–288.RPM BakM. JoenjeI. de JongDYM LambrechtsG. Nieuwland1998Bacterial suspension feeding by coral reef benthic organisms.Mar Ecol Prog Ser175285288

185. Bone Q, Carre C, Chang P (2003) Tunicate feeding filters. J Mar Biol Ass UK 83: 907–919.Q. BoneC. CarreP. Chang2003Tunicate feeding filters.J Mar Biol Ass UK83907919

186. Monniot F, Monniot C (2003) Ascidians from the outer slope and bathyal western Pacific. Zoosystema 25: 681–749.F. MonniotC. Monniot2003Ascidians from the outer slope and bathyal western Pacific.Zoosystema25681749

187. Gili JM, Coma R, Orejas C, López-González PJ, Zabala M (2001) Are Antarctic suspension-feeding communities different from those elsewhere in the world? Polar Biol 24: 473–485.JM GiliR. ComaC. OrejasPJ López-GonzálezM. Zabala2001Are Antarctic suspension-feeding communities different from those elsewhere in the world?Polar Biol24473485

188. Dayton PK, Robilliard GA, Devries AL (1969) Anchor Ice Formation in McMurdo Sound, Antarctica, and Its Biological Effects. Science 163(3864): 273–274.PK DaytonGA RobilliardAL Devries1969Anchor Ice Formation in McMurdo Sound, Antarctica, and Its Biological Effects.Science1633864273274

189. Smale DA, Brown KM, Barnes DKA, Fraser KPP, Clarke A (2008) Ice Scour Disturbance in Antarctic Waters. Science 321(5887): 371.DA SmaleKM BrownDKA BarnesKPP FraserA. Clarke2008Ice Scour Disturbance in Antarctic Waters.Science3215887371

190. Boakes EH, McGowan PJK, Fuller RA, Chang-qing D, Clark NE, et al. (2010) Distorted Views of Biodiversity: Spatial and Temporal Bias in Species Occurrence Data. PLoS Biol 8(6): e1000385.EH BoakesPJK McGowanRA FullerD. Chang-qingNE Clark2010Distorted Views of Biodiversity: Spatial and Temporal Bias in Species Occurrence Data.PLoS Biol86e1000385

191. Stachowicz JJ, Whitatch RB, Osman RW (1999) Species diversity and invasion resistance in a marine ecosystem. Science 286: 1577–1579.JJ StachowiczRB WhitatchRW Osman1999Species diversity and invasion resistance in a marine ecosystem.Science28615771579

192. Kennedy TA, Naeem S, Howe KM, Knops JMH, Tilman D, Reich P (2002) Biodiversity as a barrier to ecological invasion. Nature 417(6889): 636–638.TA KennedyS. NaeemKM HoweJMH KnopsD. TilmanP. Reich2002Biodiversity as a barrier to ecological invasion.Nature4176889636638

193. Bullard SG, Lambert G, Carman MR, Byrnes J, Whitlatch RB, et al. (2007) The colonial ascidian Didemnum sp. A: Current distribution, basic biology and potential threat to marine communities of the northeast and west coasts of North America. J Exp Mar Biol Ecol 342: 99–108.SG BullardG. LambertMR CarmanJ. ByrnesRB Whitlatch2007The colonial ascidian Didemnum sp. A: Current distribution, basic biology and potential threat to marine communities of the northeast and west coasts of North America.J Exp Mar Biol Ecol34299108

194. Rius M, Pascual M, Turon X (2008) Phylogeography of the widespread marine invader Microcosmus squamiger (Ascidiacea) reveals high genetic diversity of introduced populations and non-independent colonisations. Divers Distrib 14: 818–828.M. RiusM. PascualX. Turon2008Phylogeography of the widespread marine invader Microcosmus squamiger (Ascidiacea) reveals high genetic diversity of introduced populations and non-independent colonisations.Divers Distrib14818828

195. Hess JE, Swalla BJ, Moran P (2009) New molecular markers to genetically differentiate populations of Didemnum vexillum - an invasive ascidian species. Aquat Inv 3: 367–378.JE HessBJ SwallaP. Moran2009New molecular markers to genetically differentiate populations of Didemnum vexillum - an invasive ascidian species.Aquat Inv3367378

196. Stefaniak L, Lambert G, Gittenberger A, Zhang H, Lin S, et al. (2009) Genetic conspecificity of the worldwide populations of Didemnum vexillum Kott, 2002. Aquat Inv 4: 29–44.L. StefaniakG. LambertA. GittenbergerH. ZhangS. Lin2009Genetic conspecificity of the worldwide populations of Didemnum vexillum Kott, 2002.Aquat Inv42944

197. Stachowicz JJ, Terwin JR, Whitlatch RB, Osman RW (2002) Linking climate change and biological invasions: Ocean warming facilitates nonindigenous species invasions. Proc Natl Acad Sci USA 99: 15497–15500.JJ StachowiczJR TerwinRB WhitlatchRW Osman2002Linking climate change and biological invasions: Ocean warming facilitates nonindigenous species invasions.Proc Natl Acad Sci USA991549715500

198. Sorte CJB, Williams SL, Zerebecki RA (2010) Ocean warming increases threat of invasive species in a marine fouling community. Ecology 91: 2198–2204.CJB SorteSL WilliamsRA Zerebecki2010Ocean warming increases threat of invasive species in a marine fouling community.Ecology9121982204

199. Carlton JT, Geller JB (1993) Ecological roulette: the global transport and invasion of nonindigenous marine organisms. Science 261: 78–82.JT CarltonJB Geller1993Ecological roulette: the global transport and invasion of nonindigenous marine organisms.Science2617882