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Web End = Plant Mol Biol (2015) 89:353363 DOI 10.1007/s11103-015-0371-5
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Web End = GmmiR156b overexpression delays owering time in soybean
Dong Cao1 Ying Li2 Jialin Wang1 Haiyang Nan1 Youning Wang3
Sijia Lu1,4 Qiong Jiang3 Xiaoming Li1,4 Danning Shi1,4 Chao Fang1,4
Xiaohui Yuan1 Xiaohui Zhao1,4 Xia Li3 Baohui Liu1 Fanjiang Kong1
Received: 7 March 2015 / Accepted: 27 August 2015 / Published online: 4 September 2015 Springer Science+Business Media Dordrecht 2015
Abstract Soybean [Glycine max (L.) Merr.] is an important crop used for human consumption, animal feed and biodiesel fuel. Wering time and maturity signicantly affect soybean grain yield. In Arabidopsis thaliana, miR156 has been proposed to regulate the transition from the juvenile to the adult phase of shoot development, which is accompanied by changes in vegetative morphology and an increase in reproductive potential. However, the molecular mechanisms underlying miR156 function in soybean owering remain unknown. Here, we report that
the overexpression of GmmiR156b delays owering time in soybean. GmmiR156b may target SPL orthologs and negatively regulate GmSPLs, thereby delaying owering in soybean under LD and natural conditions. GmmiR156b down-regulates several known owering time regulators in soybean, such as GmAP1 (a, b, c), GmLFY2, GmLFY2, GmFULs, GmSOC1s, GmFT5a, and GmmiR172. These data show that a similar miR156-SPL regulatory module was conserved in the soybean owering pathway. However, GmFULs, GmSOC1a and GmSOC1b were signicantly suppressed under LD conditions but not under SD conditions, which is different in Arabidopsis that these genes were down-regulated irrespective of photoperiod. In addition, GmmiR156b was up-regulated by E1, E2 (GmGI), E3 and E4, which control owering time and maturity in soybean, and suppressed E1 (E1-Like) and E2 (E2-Like) genes under LD conditions. These data indicated that the miR156-SPL regulatory module was also with some degree of divergent in soybean owering pathway.
Keywords Soybean microRNA Flowering time
GmmiR156b
Introduction
Soybean is a facultative short-day (SD) plant. Day length has an important inuence on owering and growth habit in soybean, and responsiveness to this factor is an important production trait. Ten maturity loci, E1E9 and J, that control owering time...