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Antonie van Leeuwenhoek (2013) 103:10071014 DOI 10.1007/s10482-013-9880-x
ORIGINAL PAPER
Halalkalicoccus paucihalophilus sp. nov., a halophilic archaeon from Lop Nur region in Xinjiang, northwest of China
Bing-Bing Liu Shu-Kun Tang Yong-Guang Zhang
Xin-Hua Lu Li Li Juan Cheng Yuan-Ming Zhang
Li-Li Zhang Wen-Jun Li
Received: 1 July 2012 / Accepted: 10 January 2013 / Published online: 22 January 2013 Springer Science+Business Media Dordrecht 2013
Abstract Two extremely halophilic archaea, designated YIM 93701T and YIM 93664, were isolated from Lop Nur region in Xinjiang Province, northwest of China. The cells of the two strains were observed to be cocci, non-motile and Gram-negative. The organisms were determined to be aerobic and required at least 6 % NaCl for growth (optimum 2025 % and maximum 35 %). Growth was found to occur in the ranges of 1650 C (optimum 37 C) and pH 6.08.5 (optimum6.57.5). Cells did not lyse in distilled water. Phylo-genetic analysis based on 16S rRNA gene sequences indicated that the two strains belongs to the genus Halalkalicoccus and possessed 99.3 and 99.5 %
similarities with their closest phylogenetic relative Halalkalicoccus tibetensis JCM 11890T. Major polar lipids of the two strains were determined to be phosphatidylglycerol(PG),phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol sulfate (PGS) and three unidentied glycolipids. The DNA G?C contents were determined to be60.060.4 mol%. The DNA hybridization between the two strains was 92.0 %. In addition, the hybridizations of both strains to H. tibetensis were 49 and 52 %, respectively, and to Halalkalicoccus jeotali were 38 and 33 %, respectively. On the basis of physiological, biochemical tests and phylogenetic differentiations, strains YIM 93701T and YIM 93664 were classied as the same species which represent a novel species in the genus Halalkalicoccus, for which the name Halalkalicoccus paucihalophilus sp. nov. is proposed. The type strain is YIM 93701T (=JCM 17505T = CCT CC 2012803T).
Bing-Bing Liu and Shu-Kun Tang contributed equally to this study.
Electronic supplementary material The online version of this article (doi:http://dx.doi.org/10.1007/s10482-013-9880-x
Web End =10.1007/s10482-013-9880-x ) contains supplementary material, which is available to authorized users.
B.-B. Liu Y.-G. Zhang L. Li Y.-M. Zhang W.-J. Li
Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, 830011r}umqi, Peoples Republic of China
B.-B. Liu S.-K. Tang J....