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Aesth Plast Surg (2008) 32:698699 DOI 10.1007/s00266-008-9159-1
LETTER TO THE EDITOR
Human Adipose-Derived Stem Cells Adhere to Acellular Dermal Matrix
A. M. Altman E. S. Chiu X. Bai Y. Yan Y. H. Song R. E. Newsome E. U. Alt
Received: 7 January 2008 / Accepted: 9 January 2008 / Published online: 15 April 2008 Springer Science+Business Media, LLC 2008
Human acellular dermal matrix (ADM) is commonly used in the surgical closure of soft tissue defects not amenable to primary repair. Adipose-derived stem cell (ASC) therapy in the context of soft tissue loss may enhance local micro-vascular density and wound healing [3]. The current study aimed to determine whether ASCs adhere to ADM in vitro and whether stem cellseeded ADM is a viable approach for surgical application.
Materials and Methods
In accordance with the Tulane University Institutional Review Board, ASCs were isolated from discarded adipose tissue obtained at body-contouring procedures as described [5]. The ASCs were characterized by uorescence-activated cell sorting as previously described [1].
Acellular dermal matrix 0.4 to 0.8 mm in thickness was obtained (AlloDerm; LifeCell, Branchburg, NJ, USA, and FlexHD; MTF, Edison, NJ, USA). Passage 1 to 5 cell suspensions in growth medium were seeded into multiwell plates covered with ADM, papillary dermis side facing up, at a density of 5 9 104 cells/cm2 for histology, 7.5 9 104 cells/cm2 for quantitative studies, and...