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Arch Virol (2017) 162:299306 DOI 10.1007/s00705-016-3074-6
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Identication and characterization of an isolate of apple green crinkle associated virus involved in a severe disease of quince (Cydonia oblonga, Mill.)
Massimiliano Morelli1 Annalisa Giampetruzzi1 Lorenzo Laghezza2
Luigi Catalano2 Vito Nicola Savino3 Pasquale Saldarelli1
Received: 27 July 2016 / Accepted: 17 September 2016 / Published online: 5 October 2016 Springer-Verlag Wien 2016
Abstract A quince tree showing severe symptoms of a previously undescribed viral disease occurring in northern Apulia (Italy) was analysed using high-throughput sequencing of small RNA libraries, leading to the identication of a new strain of apple green crinkle associated virus (isolate AGCaV-CYD) showing peculiar traits. RTPCR with specic primers detected AGCaV-CYD in consistent association with symptoms in the surveyed orchards. Molecular characterization of the reconstructed genome, together with phylogenetic analysis, showed it to be closely related to an AGCaV strain causing green crinkle disease in apple (AGCaV-AUR) and divergent from the type strain of apple stem pitting virus (ASPV-PA66).
Quince (Cydonia oblonga, Mill.), a fruit tree of Asian origin, is grown and cultivated throughout the Mediterranean basin and Central-Eastern Europe to be used in jam and spirit production or as medicine and a avouring agent, as well as a valued dwarng rootstock for pear. This species has been recognized as a natural host to several viruses, known to be widely distributed in pome fruit crops,
such as apple stem pitting virus (ASPV), apple chlorotic leaf spot virus (ACLSV) and apple stem grooving virus (ASGV) [15].
The main viral disease observed so far in quince orchards in the UK [6], former Yugoslavia [7] and Greece [2] is quince fruit deformation (QFD), characterized by severe fruit deformities, esh browning, and green spots occurring in the depressed areas of the skin. Less often, QFD is accompanied by sooty ringspots on the leaves, and the disease is therefore referred to as quince sooty ringspot (QSRS) [8]. Several studies have tried to identify the causal agent of QFD, and a preliminary association has been found between the symptomatology and the isolation of a viral agent closely related to ASPV [2, 3, 7, 8], although the complete genome sequencing of this isolate has never been accomplished.
In 2014, the occurrence...