Content area
Abstract
A recombinant goatpox virus was constructed in which an enhanced green fluorescent protein gene was inserted under the control of the 11K late promoter, a guanine phosphoribosyltransferase gene was inserted under the control of the 7.5K early/late promoter, and exogenous genes were inserted into an intergenic region between loci gp_24 and gp_24.5 of the recombinant genome. Analysis of protein expression showed that LT cells infected with only the recombinant virus produced specific fluorescence. A comparative growth assay demonstrated the stability of the recombinant virus at the replication level. These results suggest that the intergenic region is not essential for replication of goatpox virus.
Details
Title
Identification of an intergenic region that is not essential for replication of goatpox virus
Author
Ma, Wenge 1 ; Wang, Fang 2 ; Yu, Li 2 ; Xue, Ying 3 ; Wang, Yan 3 ; Wei, Jie 3 ; Huang, Jiong 3 ; Fu, Zihua 3
1 Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin, People’s Republic of China (GRID:grid.38587.31); Xinjiang Academy of Animal Science, Institute of Veterinary Medicine, Ürümqi, People’s Republic of China (GRID:grid.410754.3) (ISNI:0000000417634106)
2 Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin, People’s Republic of China (GRID:grid.38587.31)
3 Xinjiang Academy of Animal Science, Institute of Veterinary Medicine, Ürümqi, People’s Republic of China (GRID:grid.410754.3) (ISNI:0000000417634106)
1 Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin, People’s Republic of China (GRID:grid.38587.31); Xinjiang Academy of Animal Science, Institute of Veterinary Medicine, Ürümqi, People’s Republic of China (GRID:grid.410754.3) (ISNI:0000000417634106)
2 Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin, People’s Republic of China (GRID:grid.38587.31)
3 Xinjiang Academy of Animal Science, Institute of Veterinary Medicine, Ürümqi, People’s Republic of China (GRID:grid.410754.3) (ISNI:0000000417634106)
Pages
1337-1341
Publication year
2010
Publication date
Aug 2010
ISSN
03048608
e-ISSN
14328798
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2695649757
Copyright
© Springer-Verlag 2010.