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© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Articular cartilage is a highly organized tissue that has a limited ability to heal. Tissue engineering is actively exploited for joint tissue reconstruction in numerous cases of articular cartilage degeneration associated with trauma, arthrosis, rheumatoid arthritis, and osteoarthritis. However, the optimal scaffolds for cartilage repair are not yet identified. Here we have directly compared five various scaffolds, namely collagen-I membrane, collagen-II membrane, decellularized cartilage, a cellulose-based implant, and commercially available Chondro-Gide® (Geistlich Pharma AG, Wolhusen, Switzerland) collagen membrane. The scaffolds were implanted in osteochondral full-thickness defects, formed on adult Wistar rats using a hand-held cutter with a diameter of 2.0 mm and a depth of up to the subchondral bone. The congruence of the articular surface was almost fully restored by decellularized cartilage and collagen type II-based scaffold. The most vivid restoration was observed 4 months after the implantation. The formation of hyaline cartilage was not detected in any of the groups. Despite cellular infiltration into scaffolds being observed in each group except cellulose, neither chondrocytes nor chondro-progenitors were detected. We concluded that for restoration of hyaline cartilage, scaffolds have to be combined either with cellular therapy or morphogens promoting chondrogenic differentiation.

Details

Title
Implantation of Various Cell-Free Matrixes Does Not Contribute to the Restoration of Hyaline Cartilage within Full-Thickness Focal Defects
Author
Ibragimova, Shabnam I 1 ; Medvedeva, Ekaterina V 1 ; Romanova, Irina A 2 ; Istranov, Leonid P 1 ; Istranova, Elena V 1 ; Lychagin, Aleksey V 3   VIAFID ORCID Logo  ; Nedorubov, Andrey A 4 ; Timashev, Peter S 1   VIAFID ORCID Logo  ; Telpukhov, Vladimir I 5 ; Chagin, Andrei S 6 

 Institute of Regenerative Medicine, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, Russia; [email protected] (S.I.I.); [email protected] (E.V.M.); [email protected] (L.P.I.); [email protected] (E.V.I.); [email protected] (P.S.T.) 
 World-Class Research Center, Digital Biodesign and Personalized Healthcare, Sechenov University, 8-2 Trubetskaya St., 119991 Moscow, Russia; [email protected] 
 Department of Traumatology, Orthopedics and Disaster Surgery, Sechenov University, 8-2 Trubetskaya St., 119991 Moscow, Russia; [email protected] 
 Center for Preclinical Research, Institute of Translational Medicine and Biotechnology, I.M. Sechenov First Moscow State Medical University, 119991 Moscow, Russia; [email protected] 
 Department of Operative Surgery and Topographic Anatomy, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, Russia 
 Institute of Regenerative Medicine, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, Russia; [email protected] (S.I.I.); [email protected] (E.V.M.); [email protected] (L.P.I.); [email protected] (E.V.I.); [email protected] (P.S.T.); Department of Physiology and Pharmacology, Karolinska Institutet, 17177 Stockholm, Sweden 
First page
292
Publication year
2022
Publication date
2022
Publisher
MDPI AG
ISSN
16616596
e-ISSN
14220067
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2618239801
Copyright
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.