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ISSN 0006 2979, Biochemistry (Moscow), 2008, Vol. 73, No. 7, pp. 826 832. Pleiades Publishing, Ltd., 2008.
Original Russian Text E. A. Gunter, O. M. Kapustina, O. V. Popeyko, T. I. Chelpanova, E. A. Efimtseva, Yu. S. Ovodov, 2008, published in Biokhimiya, 2008, Vol. 73, No. 7, pp. 1023 1031.
Induction of 1,3 Glucanase in Callus Cultures in vitro
E. A. Gunter*, O. M. Kapustina, O. V. Popeyko, T. I. Chelpanova,E. A. Efimtseva, and Yu. S. Ovodov
Institute of Physiology, Komi Research Center, Ural Branch of Russian Academy of Sciences,ul. Pervomaiskaya 50, 167982 Syktyvkar, Russia; fax: (8212) 241 001; E mail: [email protected]
Received July 2, 2007 Revision received October 16, 2007
AbstractSodium salicylate (NaSA) increased induction of both intracellular and extracellular 1,3 glucanases in callus es of campion and duckweed. NaSA concentrations from 30 to 100 mM were optimal for induction of intracellular glu canase in the campion callus, and for induction of extracellular glucanase the optimal concentration varied from 5 to 100 mM. The glucanase activity in the duckweed callus was lower than in the campion callus, and co cultivation of the cam pion callus with Trichoderma harzianum mycelium increased the production of intracellular and extracellular 1,3 glu canases and polygalacturonase in the callus. Biosynthesis by T. harzianum of glucanases, extracellular polygalacturonase and xylanase, and of intracellular galactosidase was increased. The co cultivation was accompanied by increased activity of intracellular acidic isoform of glucanase Glu 3 secreted by the callus cells into the medium, whereas NaSA activated in the callus culture the extracellular acidic isoform Glu 1 and extracellular basic isoform Glu 5. These data indicate the induc tion of these isoforms and the specificity of protective response of plant cells to different factors.
DOI: 10.1134/S0006297908070110
Key words: Silene vulgaris (M.) G., Lemna minor L., Trichoderma harzianum, callus, 1,3 glucanase, polygalacturonase, sodium salicylate
Plants respond to infection with phytopathogenic fungi by triggering complex defense mechanisms [1]. The defense mechanisms include modification of cell walls (lignification and formation of callose), production of reactive oxygen species, and biosynthesis of phytoalexins and pathogenesis related proteins (PR proteins) [1 3]. PR proteins are represented by chitinases and 1,3 glu canases capable of hydrolyzing chitin and 1,3 glucans, which are structural components of cell walls of phy topathogens and...