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Abstract
Eleven species of local plants have been found as a potential antibacterial according to the community of all ethnic groups in Kartanegara Regency. However, inhibitory tests have not been carried out on test bacteria. The aim of the study is to classify the level of ability of local plant extracts in inhibiting the growth of Staphylococcus aureus and Escherichia coli. The research samples were local plant parts, namely Senna alata, Psidium guajava, Bryophyllum pinnatum, Cymbopogon citratus, Piper betle, Curcuma longa, Zingiber afficinale, Curcuma aeruginosa, Euleutherine bulbosa, Citrus aurantifolio, Ricinus communis which were potential antibacterial, obtained in Kutai Kartanegara Regency area. The research instrument was in the form of laboratory instruments for making extracts for phytochemical analysis and inhibitory tests on test bacteria, and the ruler. The dilution level of local plant extracts used are 1 mg/ml, 0.5 mg/ml, and 0.25 mg/ml, 50 mg/ml, and pure extract. The test bacteria used were Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 35218. Determination of inhibition was done by measuring the area of clear that arises after the incubation process. The data analysis technique used percentage. The results of a data analysis showed that the percentage of level of inhibitory ability of local plant extracts on growth of Staphylococcus aureus and Escherichia coli showed the same data, in the dilution levels of 0.25, 0.5, 1, 50, all of which were 100% not effect; at the pure level is 9.09% no effect, 36.37% weak, 45.46% middle, 9.09% strong, and 0% very strong. The conclusion of the study was classified based on the level of ability of local plant extracts are no effect, weak, middle, and strong. Future research in the future needs to be done mainly focused on increasing the obtain area of local plant parts, and types of test bacteria to obtain more information.
Keywords: inhibition ability, local plants extracts, Stahylococcus aureus, Escherichia coli
INTRODUCTION
The results of survey, was found that the people in Kutai Kartanegara Regency consisted of many ethnicities (multiethnic). According to the community's recognition of all ethnicities surveyed, there are 11 local plant species that have the potential to be antibacterial. The eleven species of plants are Senna alata, Bryophyllum pinnatum, Citrus aurantifolio, Cymbopogon citratus, Zingiber afficinale, Piper betle, Curcuma aeruginosa, Ricinus communis...