Abstract

The International Society for the Clinical Electrophysiology of Vision (ISCEV) standard for full-field electroretinography (ERG) describes a minimum set of tests, but encourages the use of additional protocols for clinical ERG testing. This extended protocol describes recording methods and derivations that will allow analysis of rod-driven components of the dark-adapted (DA) strong flash ERG a-wave, more closely related to rod phototransduction than ISCEV standard DA ERGs. The method involves recording ERGs to a flash strength equivalent to 30 cd s m2 under conditions of dark adaptation and additionally to the same stimulus following light adaptation (LA) and in the presence of a standard photopic background luminance of 30 cd m−2. The isolated rod-driven ERG a-wave is derived by subtracting the LA response from the DA ERG. The method is likely to be of value in the characterization of retinal disorders which affect rod quantal catch, diseases that affect the dynamics of any component of the activation phase of rod phototransduction, or those affecting total numbers of rod photoreceptors.

Details

Title
ISCEV extended protocol for derivation and analysis of the strong flash rod-isolated ERG a-wave
Author
Mitchell, Brigell 1   VIAFID ORCID Logo  ; Jeffrey, Brett G 2 ; Mahroo, Omar A 3 ; Radouil, Tzekov 4 

1  Aerpio Therapeutics, Cincinnati, USA (GRID:grid.417420.4) 
2  National Eye Institute, Ophthalmic Genetics and Visual Function Branch, Bethesda, USA (GRID:grid.280030.9) (ISNI:0000 0001 2150 6316) 
3  Moorfields Eye Hospital, London, UK (GRID:grid.439257.e) (ISNI:0000 0000 8726 5837); University College London, Institute of Ophthalmology, London, UK (GRID:grid.83440.3b) (ISNI:0000000121901201) 
4  University of South Florida, Department of Ophthalmology, Tampa, USA (GRID:grid.170693.a) (ISNI:0000 0001 2353 285X); University of South Florida, Department of Medical Engineering, Tampa, USA (GRID:grid.170693.a) (ISNI:0000 0001 2353 285X) 
Pages
5-12
Publication year
2020
Publication date
Feb 2020
Publisher
Springer Nature B.V.
ISSN
00124486
e-ISSN
15732622
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1007/s10633-019-09740-4
ProQuest document ID
2343250143
Copyright
Documenta Ophthalmologica is a copyright of Springer, (2020). All Rights Reserved. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.