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Secondary metabolites of microbial origin, either as pure compounds or crude extracts, provide opportunities for new drug development because of their chemical diversity. The aim of the present study is to isolate Molecular characterization and extract the bioactive compounds from the fungus of A. quadrilineatus (OL979151) in the rhizospheric soil of sunflower and mustard crop fields in Davanagere region. Dilution plate technique was used to isolate the rhizosphere fungi. The total genomic DNA was extracted from isolate of Aspergillus quadrilineatus based on Cetrimide Tetradecyl Trimethyl Ammonium Bromide (CTAB). Mass production of the fungal mat of A. quadrilineatus was established on potato dextrose broth medium. The dried methanol extract of fungus was subjected to HR-LCMS analysis; Methanol extract of A. quadrilineatus fungus shows that the compounds Amobarbital, Pirbuterol, Bambuterol, Cilazaprilat, Oxotremorine, Aminopyrine and Oryzanol as the major constituents. The compounds exhibit analgesic, anti-inflammatory, and antipyretic properties and various therapeutic uses.
Keywords: Aspergillus quadrilineatus, Methanol extract, HR-LCMS, Rhizosphere.
INTRODUCTION
Soil is the major component of earth's ecosystem which comprises of organic matter, minerals, gases and large number of micro and macro organisms (Chandrasekhar et al., 2014). Microorganisms are helpful in increasing the soil fertility and plant growth as they are involved in several biochemical transformation and mineralization activities in soils (Turukmane et al., 2018). Rhizosphere is the narrow zone of soil surrounding the root where microbe population are stimulated by root activities. Rhizosphere is known to be a hotspot of microbial activities (Chandrasekhar et al., 2014). The microorganisms in the rhizosphere play a great biological role in the growth of host plant. Microorganisms in the rhizosphere complete both chemical and physical modifications to the soil profile in and around rhizosphere that affect plants. This occurs through the defence mechanism provided by the rhizosphere microbial communities against pathogens or through providing nutrition to the plant by their role in mineralization of different organic compounds (Raha and Shagufta, 2019). Plant age as well as plant type are influential in exudates release and impact on microflora in rhizosphere zone (Deshmukh et al., 2013).
Secondary metabolites are compounds produced by an organism that are not required for primary metabolic processes. In soil habitat, fungi compete against different other microorganisms and may induce antimicrobial production. Filamentous fungi contaminated with broad spectrum...