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Curr Microbiol (2017) 74:702709
DOI 10.1007/s00284-017-1231-0
Received: 3 August 2016 / Accepted: 14 March 2017 / Published online: 23 March 2017 Springer Science+Business Media New York 2017
standard and fungal taxols. As fungal taxol was produced simpler than other methods and could signicantly aect viability and specic genes expression prole, it is recommended that using of taxol-producing fungi from Iranian yew could be a safe and condent procedure to overcome challenges of using other methods.
Keywords Endophytic fungi Iran Taxol Colony-
PCR Human cancer
Introduction
A medicinal plant is any plant which in some of its organs contains substances that can be used for therapeutic purposes. For example, medicinal plant species of Taxus harbor endophytic fungi in various parts such as leaves and stems [20]. Many dierent species of Taxus species are distributed worldwide. Taxus baccata L. species is found in the temperate forests of northern Iran (at Hyrcanian region) with an origin of tertiary geological time period. Recently, all Iranian Taxus baccata species are protected within the nature reserves [5].
Taxol, also known as paclitaxel, a chemical substance of tetracyclic diterpene lactam was rst isolated from the bark of the Pacic yew Taxus brevifolia [25]. The paclitaxel molecule consists of a tetracyclic core called baccatin III and an amide tail. The core rings are conveniently called ring A (a cyclohexene), ring B (a cyclooctane), ring C (a cyclohexane), and ring D (an oxetane) [6]. Taxol is an important anti-cancer drug approved by the FDA in human with a specic mechanism of action. It can inhibit de-polymerization of microtubules during cell-cycle, cell growth but initiate cell apoptosis [3]. Taxus species are inefficient and environmentally
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Web End = Isolation ofTaxol-Producing Endophytic Fungi fromIranian Yew Through Novel Molecular Approach andTheir Eects onHuman Breast Cancer Cell Line
AbdollahKasaei1 MohsenMobini-Dehkordi1,2 ForuzandehMahjoubi3
BehnazSaar1,2
Abstract Taxol or paclitaxel, an approved drug by the
Food and Drug Administration, is being used for the treatment of human cancers. This study aimed to isolate and determine dierent species of native endophytic fungi from Iranian Taxus baccata (yew) plants located in the northern forests of Iran. To do so, a novel molecular screening approach was performed for 50 isolated endophytic fungi through amplication of exon No. 1 of taxadine...