Content area
Full Text
Abstract:
Background:
Cementum is a least understood hard tissue. Some authors consider it to be a variant of bone while others do not. It is gaining much attention these days much as it plays an important role in periodontal regeneration. There are not much employed methods to standardise studies involving cementum.
Aim:
The present study aims to study and compare the morphology of cementum using different histological stains under light microscopy.
Materials and methods:
Extracted and decalcified human Teeth were stained using three different histologic stains H and E, Alcian Blue- NFR and toluidine blue and observed under light microscope.
Results:
Hematoxylin and eosin staining staining of cementum with eosinophilc staining of dentin. Under toluidine blue staining,cementum stained deep blue in contrast to the underlying dentin which stained pale blue. Alcian blue- NFR stain showed pink staining of Cementum and underlying dentin also the lacunae in the cellular cementum showed blue staining There was adequate contrast between the cementum and dentin in all the three stains. There was no significant difference in the stainig intensity between cellular and acellular cementum. The incremental lines were better observed in H and E and toluidine blue staining.
Conclusion:
These findings underscore the fact that cementum is rich in extracellular matrix proteins but lacks significant proteoglycan content which is usually restricted to the lacunae and canaliculi.
Key words: Cementum, Hematoxylin and eosin, Alcian blue, Nuclear fast red, Toluidine blue O
INTRODUCTION:
The cementum is a thin, mineralized tissue covering the root dentin.1 It is avascular, inorganic, dense, inert, extremely narrow layer of tissue that provides attachment of the tooth to the periodontal ligament.2 It also has reparative and adaptive functions. It also helps in maintaining the integrity of root surface and supports tooth.3 Cementum is of two types acellular extrinsic fiber cementum or acellular or primary cementum and cellular intrinsic fiber cementum or cellular or secondary cementum.1 Cementum is of two types acellular extrinsic fiber cementum or acellular or primary cementum and cellular intrinsic fiber cementum or cellular or secondary cementum.It is present primarily as acellular cementum on the cervical root and helps in tooth attachment to adjacent PDL and cellular cementum covering the apical root helps in post eruption tooth movement and adaptation to occlusion.1,4 cementum...