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Antonie van Leeuwenhoek (2013) 104:423430 DOI 10.1007/s10482-013-9967-4
ORIGINAL PAPER
Litorilituus sediminis gen. nov. sp. nov., isolated from coastal sediment of an amphioxus breeding zone in Qingdao, China
Yan Wang Rui Zhao Shiqi Ji Zhao Li
Tong Yu Bingyu Li Xiaochong Shi
Xiao-Hua Zhang
Received: 21 May 2013 / Accepted: 28 June 2013 / Published online: 10 July 2013 Springer Science+Business Media Dordrecht 2013
Abstract A Gram-negative, non-spore-forming, catalase- and oxidase-positive, aerobic, curved-rod shaped bacterium with polar or subpolar agellum, designated strain JYr2T, was isolated from a sediment sample collected from an amphioxus breeding zone in the coastal region of Qingdao, China. The organism grew optimally at 37 C, pH 89 and in the presence of 3 % (w/v) NaCl or 34 % sea salts (w/v; Sigma). Salt was required for growth. The strain contained isoprenoid quinone 8 (Q-8, 100 %) as the predominant isoprenoid quinone and C16:0 (24.1 %) and
C16:1x7c and/or C16:1x6c (35.8 %) as major fatty acids. Phosphatidylethanolamine, phosphatidylglycerol, aminophospholipid (PN) and two aminolipid
(AL1, AL2) were the major constituents of the phospholipids. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JYr2T formed a distinct evolutionary lineage within the family Colwelliaceae. It showed \95.0 % sequence similarities to all validly published species in the family Colwelliaceae, except 95.2 % to Thalassomonas viridans DSM 13754T and 95.0 % to Colwellia polaris JCM 537T. The G?C content of the DNA was42.7 mol%. On the basis of the polyphasic taxonomic study, strain JYr2T (= CGMCC 1.10794T = JCM 17549T) was considered to represent a novel genus and species in Gammaproteobacteria, for which the name Litorilituus sediminis gen. nov. sp. nov. was proposed.
Keywords Litorilituus sediminis gen. nov. sp. nov.
Taxonomy Amphioxus breeding zone 16S rRNA
Introduction
The family Colwelliaceae, which belongs to the Gammaproteobacteria, was proposed by Ivanova et al. (2004) for gram-negative, straight or curved rods, chemo-organotrophic and halophilic bacteria. At the time of writing, the family Colwelliaceae contains the genera Thalassomonas and Colwellia (Ivanova et al. 2004), representatives of which occur in the marine environment (Yu et al. 2011; Park et al. 2011; Choi et al. 2010; Hosoya et al. 2009; Zhang et al. 2008;
The GenBank accession number for the 16S rRNA gene sequence of Litoribacillus peritrichatus JYr2T is...