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Web End = Theor Appl Genet (2015) 128:20852098 DOI 10.1007/s00122-015-2569-y
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Web End = Loci, genes, and mechanisms associated with tolerance to ferrous iron toxicity in rice (Oryza sativa L.)
Elsa Matthus1 LinBo Wu1 Yoshiaki Ueda1 Stefanie Hller1 Mathias Becker1 Michael Frei1
Received: 22 April 2015 / Accepted: 16 June 2015 / Published online: 8 July 2015 Springer-Verlag Berlin Heidelberg 2015
exceeding a signicance threshold of log10 P > 4.0,
which represented 18 distinct loci. One locus mapped for foliar symptom formation on chromosome 1 contained two putative glutathione-S-transferases, which were strongly expressed under iron stress and showed sequence polymorphisms in complete linkage disequilibrium with the most signicant SNP. Contrasting haplotypes for this locus showed signicant differences in dehydroascorbate reductase activity, which affected the plants redox status under iron stress. We conclude that maintaining foliar redox homeostasis under iron stress represented an important tolerance mechanism associated with a locus identied through GWAS.
Introduction
Iron (Fe) toxicity is one of the most commonly observed mineral disorders in rice production affecting millions of hectares of rice land, especially in Southeast Asia and West Africa (Audebert and Fofana 2009; Becker and Asch 2005). It specically affects rice production, because the low soil redox potential of ooded rice paddies leads to the prevalence of the reduced and soluble Fe2+ (ferrous), as opposed to well aerated soils, in which sparingly soluble forms of oxidized Fe3+ (ferric) are dominant. Fe toxicity can occur in variable soil types, including acid sulfate or acid clay soils with inherently high Fe concentrations, or poorly drained sandy soils in valleys receiving Fe-rich runoff water from adjacent slopes (Becker and Asch 2005). Fe toxicity causes substantial yield losses in rice and can lead to complete crop failure in severe cases.
Fe is an important plant nutrient and its enrichment in the grain is desirable from a human nutrition perspective (White and Broadley 2009), but foliar tissue concentrations
Abstract
Key message A genomewide association study in rice yielded loci and candidate genes associated with tolerance to iron toxicity, and revealed biochemical mechanisms associated with tolerance in...