Marker-assisted introgression of a QTL region to improve rust resistance in three elite and popular varieties of peanut (Arachis hypogaea L.)

Theor Appl Genet (2014) 127:17711781 DOI 10.1007/s00122-014-2338-3

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Markerassisted introgression of a QTL region to improve rust resistance in three elite and popular varieties of peanut (Arachis hypogaea L.)

Rajeev K. Varshney Manish K. Pandey Pasupuleti Janila Shyam N. Nigam Harikishan Sudini M. V. C. Gowda Manda Sriswathi T. Radhakrishnan Surendra S. Manohar Patne Nagesh

Abstract

Key message Successful introgression of a major QTL for rust resistance, through markerassisted backcross ing, in three popular Indian peanut cultivars generated several promising introgression lines with enhanced rust resistance and higher yield.

Abstract Leaf rust, caused by Puccinia arachidis Speg, is one of the major devastating diseases in peanut (Arachis hypogaea L.). One QTL region on linkage group AhXV explaining upto 82.62 % phenotypic variation for rust resistance was validated and introgressed from cultivar GPBD 4 into three rust susceptible varieties (ICGV 91114, JL 24 and TAG 24) through marker-assisted backcrossing (MABC). The MABC approach employed a total of four markers including one dominant (IPAHM103) and three

Communicated by David A. Lightfoot.

Electronic supplementary material The online version of this article (doi:http://dx.doi.org/10.1007/s00122-014-2338-3

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R. K. Varshney (*) M. K. Pandey P. Janila S. N. Nigam H. Sudini M. Sriswathi S. S. Manohar P. Nagesh

International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad 502324, Indiae-mail: [email protected]

R. K. Varshney

CGIAR Generation Challenge Programme (GCP), c/o CIMMYT, 06600 Mexico, DF, Mexico

M. V. C. Gowda

University of Agricultural Sciences (UAS), Dharwad 580005, India

T. Radhakrishnan

Directorate of Groundnut Research (DGR), Junagadh 362001, India

Received: 5 July 2013 / Accepted: 22 May 2014 / Published online: 14 June 2014 The Author(s) 2014. This article is published with open access at Springerlink.com

co-dominant (GM2079, GM1536, GM2301) markers present in the QTL region. After 23 backcrosses and selng, 200 introgression lines (ILs) were developed from all the three crosses. Field evaluation identied 81 ILs with improved rust resistance. Those ILs had signicantly increased pod yields (5696 %) in infested environments compared to the susceptible parents. Screening of selected 43 promising ILs with 13 markers present on linkage group AhXV showed introgression of the target QTL region from the resistant parent in 11 ILs.Multi-location eld evaluation of these ILs...