Mesenchymal stem cell transplantation improves chronic colitis-associated complications through inhibiting the activity of toll-like receptor-4 in mice

Abstract

[...]the pathogenesis of hepatobiliary complications is still unclear. [...]it is important to investigate the mechanism of chronic colitis-associated hepatobiliary complications. The primers were designed as: TNF-α-forward 5′-GGA AAG GAC GGA CTG GTG TA-3′, TNF-α-reverse 5’-TGC CAC TGG TCT GTA ATC CA-3′; IFN-γ-forward 5’-GGC AAG TTC AAC GGC ACA G-3′, IFN-γ-reverse 5’-CGC CAG T AG ACT CCA CGA CAT-3′; IL-1β-forward 5′-G AGC ACC TTC TTT TCC TTC ATC TT-3′, IL-1β-reverse 5’-TCA CAC ACC AGC AGG TTA TCA TC-3′; IL-17A-forward 5′-GGA AAG GAC GGA CTG GTG TA-3′, IL-17A-reverse 5′-TG C CAC TGG TCT GTA ATC CA-3′; TLR4-forward 5′-TTT ATT CAG AGC CGT TGG- 3′ TLR4-reverse 5’-CCC ATT CCA GGT AGG TGT-3′; TRAF6-forward 5′-GTA TC C GCA TTG AGA AGC-3′, TRAF6-reverse 5’-GCA GTG AAC CAT CCG TGT-3′; NF-κB-forward 5′-AAG GAT TCG AGC AGT TAG-3′, NF-κB-reverse 5′-AAG AGT TGG TGA TAG GCT-3′; GAPDH-forward 5’-GGC AAG TTC AAC GGC ACA G-3′, and GAPDH-reverse 5’-CGC CAG TAG ACT CCA CGA CAT-3′. Concurrently, histological signs of liver damage appeared to be less severe after MSCs treatment. [...]was there any relationship between chronic colitis-associated hepatobiliary complications and colonic inflammation? [...]this data suggested that increased LPS could aggravate liver injury, probably through the inhibition of TLR4 pathway during the process of IBD.