Metal-Free C-H Alkyliminylation and Acylation of Alkenes with Secondary Amides

Abstract

Carbon-carbon bond formation by metal-free cross-coupling of two reactants with low reactivity represents a challenge in organic synthesis. Secondary amides and alkenes are two classes of bench-stable compounds. The low electrophilicity of the former and low nucleophilicity of the latter make the direct coupling of these two partners challenging yet highly desirable. We report herein an unprecedented intermolecular reaction of secondary amides with alkenes to afford α,β-unsaturated ketimines or enones, which are versatile intermediates for organic synthesis and are prevalent in bioactive compounds and functional materials. Our strategy relies on the chemoselective activation of the secondary amide with trifluoromethanesulfonic anhydride (Tf₂ O)/2-fluoropyridine to generate a highly reactive nitrilium intermediate, which reacts efficiently with alkenes. This metal-free synthesis is characterized by its mild reaction conditions, excellent functional group tolerance and chemoselectivity, allowing the preparation of multi-functionalized compounds without using protecting groups.

Metal-Free CH Alkyliminylation and Acylation of Alkenes with Secondary Amides

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Pei-Qiang Huang, Ying-Hong Huang, Hui Geng & Jian-LiangYe

Carboncarbon bond formation by metal-free cross-coupling of two reactants with low reactivity represents a challenge in organic synthesis. Secondary amides and alkenes are two classes of bench-stable compounds. The low electrophilicity of the former and low nucleophilicity of thelatter make the direct coupling of these two partners challenging yet highly desirable. We report ,-unsaturated ketimines or enones, which are versatile intermediates for organic synthesis and are prevalent in bioactive compounds and functional materials. Our strategy relies on the chemoselective

free synthesis is characterized by its mild reaction conditions, excellent functional group tolerance and chemoselectivity, allowing the preparation of multi-functionalized compounds without using protecting groups.

Organic chemistry is the chemistry of carbon compounds. Thus carboncarbon (CC) bond-forming reactions occupy the central position in organic synthesis. Most of these reactions are polar1 by nature and involve the reaction of a nucleophile with an electrophile. The direct reaction of a weak nucleophile with a weak electrophile (Nuw-Elw) is difficult under conventional reaction conditions. Alkenes are a class of stable and easily available weak -nucleophiles, which can only undergo transition metal-mediated CC bond forming reactions, or react with reactive electrophiles such as acyl chlorides (Friedel-Cras acylation of alkenes)2 or in situ generated highly electrophilic intermediates, such as nitrilium ions [the extended BischlerNapieralski (BN) reaction37], iminium8/N-acyliminium ions9, and acid-activated aldehydes/ketones (Prins reaction10). On the other hand, although nitrilium ions are key intermediates in several classical reactions such as Houben-Hoesch11, Ritter12,13, von Braun13, Bischler-Napieralski13, Beckmann13, Schmidt13, and Ugi reactions14, their participation in synthetically useful intermolecular reactions with alkenes is unknown. Amides are another class of bench-stable compounds with low electrophilicity15,16 due to the strong resonance between the * orbital of the carbonyl group and the nitrogen lone pair. It is thus challenging to couple alkenes with amides, especially secondary amides because of the acidic proton on the N-atom. As a result, only isolated examples of intramolecular coupling reactions under harsh conditions are known (Fig.1a). An efficient intermolecular cross-coupling reaction of alkenes with secondary amides remains elusive (Fig.1b). However, such a transformation would be highly useful considering the widespread use of secondary amides as intermediates in organic synthesis and the requisite conversion of these species into other classes of compounds16,17 at lower oxidation states15,1824 as well as the versatility of ,-unsaturated ketimines (enimines)25 and ,-unsaturated ketones (enones) in organic synthesis, medicinal chemistry26, and molecular switches27.

In response to this challenge, we report herein a metal-free intermolecular coupling reaction of secondary amides with alkenes to afford multi-functionalized ,-unsaturated ketimines and enones (Fig.1b). Importantly, with the use of triuoromethanesulfonic (triic) anhydride (Tf2O) as a powerful yet chemoselective amide-activating reagent, the reactions are conducted under mild conditions and tolerate a host of sensitive functional groups in both the nucleophilic (alkenes) and electrophilic (secondary amides) reaction partners.

Department of Chemistry and The Key Laboratory for Chemical Biology of Fujian Province, iChEM (Collaborative Innovation Center of Chemistry for Energy Materials), College of Chemistry and Chemical Engineering, Xiamen

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Figure 1. Methods for direct CH functionalization of alkenes. (a) Classical BischlerNapieralski cyclization (BN cyclization) and SchniderHellerbachs extended BN cyclization. (b) Our metal-free Tf2O-mediated

CH functionalization of alkenes with secondary amides.

Figure 2. Research design. Strategy for the bimolecular coupling of two highly stable reaction partners: alkenes and secondary amides.

Results and Discussion

Reaction design. To realize the direct cross-coupling of an alkene with a secondary amide, it is necessary to activate one of the reaction partners. Inspired by the BN reaction, we opted for the in situ activation of the amide group. Considering the low efficiency of the classical amide activators such as P2O5 and POCl33,4,10, highly electrophilic triuoromethanesulfonic (triic) anhydride (Tf2O)28 was selected for our purpose. Tf2O in combination with a base such as 2,6-di-tert-butyl-4-methylpyridine (DTBMP)29, Hnig base30, 2-chloropyridine31, 2-uoropyridine3236, 2-iodopyridine37, 2,4,6-collidine3841, and 3-cyanopyridine42 had been employed for the activation of amides in various CC bond-forming reactions. A secondary amide 1, once treated with Tf2O, would generate a highly reactive nitrilium intermediate A32,3436,43 (Fig.2). The latter could then be captured by an alkene to give

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Figure 3. Optimization of the reaction conditions. aIsolated yield. bThe E/Z ratio of imine was determined by

1H NMR. Tf2O= triuoromethanesulfonic anhydride. 2,6-lutidine=2,6-dimethylpyridine. DTBMP=2,6-di-tert-butyl-4-methylpyridine.

an enimine 2 aer CC bond formation and deprotonation. Hydrolysis of the enimine 2 would aord enone 3 in one-pot from amide 1.

Optimization of reaction conditions. To avoid possible side reactions such as 1,5-hydride migration reaction36, the amides 1 bearing a N-2,6-dimethylphenyl group were designed as substrates for the investigation (Fig.3). At the outset of our studies, base-free amide activation protocol was attempted. To our delight, successive treatment of a solution of secondary amide 1a (1.0 equiv) in CH2Cl2 (0.25 M) with Tf2O (1.1 equiv) at 0 C for 10min and then styrene (3.0 equiv) at room temperature for 2h produced the desired ,-unsaturated ketimine 2a in 51% yield as a mixture of E/Z isomers in a ratio of 5.5:1 (entry 1). The stereochemistry of major geometric isomer was determined as E by NOESY technique (cf. Supplementary Figure 53). Note that Tf2O failed to promote the BN cyclization reaction in the absence of a base unless highly electron-rich substrates were used44.

Encouraged by this result, the eects of base were surveyed. Among the bases screened, 2-F-pyridine was found to be the best (entries 39). Under these conditions, the amount of styrene could be reduced to 1.2 equiv without aecting the reaction efficiency (entries 10 and 11). The optimal conditions were thus dened as successive treatment of a solution of secondary amide 1a (1.0 equiv) and 2-uoropyridine (1.2 equiv) in CH2Cl2 (0.25 M) with

Tf2O (1.1 equiv) at 0 C for 10 min, and then with styrene (1.2 equiv) at room temperature or 40 C for 2 h. The reaction mixture was concentrated without work-up and subjected to ash chromatographic purication to give ,-unsaturated ketimine 2a.

Substrate scope of the direct CH alkyliminylation. With optimized conditions in hand, the coupling reactions of a series of N-(2,6-dimethylphenyl)benzamides 1 with a number of alkenes were investigated (Fig.4). Styrene bearing electron-donating groups (Me, OMe) and electron-withdrawing halogens (Br, Cl, F) reacted smoothly to give the corresponding enimines in excellent yields (2b2f, 8899% yields), demonstrating superior reactivity compared with reported methods. -Methylstyrene and -phenylstyrene were also competent substrates (2g, 2h). Gratifyingly, the reaction was also compatible with the use of di- and trisubstituted aliphatic alkenes and 1,3-dienes (2i2l). The reaction of 2-methyl-2-butene produced non-conjugated ,-unsaturated ketimine 2k. Further investigation revealed that the reaction was rather insensitive to the electronic properties of the benzamide derivatives and tolerated electron-donating groups such as methyl group (2m) and methoxy group (2n), as well as the highly electron-withdrawing nitro group (2p, 2q).

The current reaction is characterized by its broad tolerance of sensitive functional groups including bromo (2o), nitro (2p, 2q), ester (2r, 2w), ketone (2s, 2z), aldehyde (2t), cyano (2u), azido (2x), tertiary amide (2v, 2y), sulfonamide (2aa), phenol (2ab) and silyl ether groups (2ac), many of which are not compatible with organometallic reagents. The highly functionalized products were all obtained in good to excellent yields, demonstrating great potential for the Tf2O-promoted method in the synthesis of complex structures. Interestingly, p-vinylstyrene

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Figure 4. Metal-free direct coupling of N-2,6-dimethylbenzamides with alkenes to give ,-unsaturated ketimines 2. aReaction conditions: Amide (1.0 equiv), 2-F-Pyr. (1.2 equiv), CH2Cl2 (0.25M), then 0C, Tf2O(1.1 equiv), 10min. Alkene (1.2 equiv), 2h. bIsolated yield. cReaction ran at room temperature (rt). dReaction ran at 40C. eThe E/Z ratio of imines was determined by 1H NMR. fThe structure was determined by X-Ray analysis (cf. Supplementary Figure 54). g2.5 equiv of amide 1f and 1.0 equiv of 1,4-divinylbenzene were used. Ts=4-toluenesulfonyl, TBS=tert-butyldimethylsilyl.

could react selectively at one end giving 2ad in 87% yield, or at both alkenes leading to 2ae in 85% yield. Finally, the reaction could be scaled up to 20 mmol-scale without yield loss as demonstrated by the reaction of 1a with styrene (2a, yield: 95%, 5.91 g, Fig. 4).

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Figure 5. Metal-free direct coupling of benzamide derivatives with alkenes to give enones 3. aReaction conditions: Amide (1.0 equiv), 2-F-Pyr. (1.2 equiv), CH2Cl2 (0.25 M), then 0C, Tf2O (1.1 equiv), 10min.

Alkene (1.2 equiv), 2h. EtOH and 3 M HCl, reux. bIsolated yield. cReaction ran at room temperature (rt).

dReaction ran at 40C.

Substrates scope of the direct CH acylation. We then turned our attention to the synthesis of enones by in situ hydrolysis of the ketimine products. Aer the Tf2O/2-uoropyridine-mediated dehydracoupling, the reaction mixture was concentrated and heated to reux in a mixture of ethanol and 3M HCl (1:1, v/v) to aord the desired enones (Fig.5). Functionalized chalcones 3c3h were synthesized in good to excellent yields by employing styrenyl alkenes and benzamide derivatives as substrates. Aliphatic and ,-unsaturated amides were also excellent substrates (3i3k, 3m). N-Alkyl amides are valuable directing group for both classical lithiation-functionalization45 and modern CH functionalization reactions4649. As a result, the transformation of the functionalized amide products obtained in these reactions into other classes of compounds are imperative. To demonstrate the value of our method in this context, the N-methyl amides 1t45, 1u48 and 1v49, which were previously obtained through transition-metal-catalyzed CH activation, were converted into the corresponding enones 3n3p in 6073% yields.

Synthetic applications. To demonstrate the synthetic potential of the enone synthesis, the coupling reaction of styrene with (S)-N-methyl-tetrahydro-5-oxo-2-furaneamide (1w), readily available in 99% ee from L-glutamic acid50, was undertaken (Fig.6a). To our delight, the desired enone (S)-3q was obtained in 70% yield without racemization (cf. Supplementary Figure 55). Multi-functionalized lactone-enones like 3q are versatile building blocks for the synthesis of bioactive natural products51.

The synthetic utility was further demonstrated by the synthesis of okanin (4) (Fig.6b), a natural product that has been found in various folk medications used in China and Korea for treating inammation, malaria, hyper-tension, diabetes, snake bite and smallpox52. The amide 1x, prepared in one step from commercially available 2,3,4-trimethoxybenzoic acid by amidation using Yes coupling reagent53 (cf. Supplementary Figure 60), reacted smoothly with 3,4-dimethoxystyrene to aord enone 3r in 86% yield. Exhaustive demethylation using BBr3 furnished okanin (4) in 84% yield.

Mechanistic investigation. To provide some experimental proofs for the presumed intermediacy of a highly electrophilic nitrilium ion, a series of NMR experiments were carried out. Secondary amide 1p was chosen for the mechanistic studies and base-free amide activation with Tf2O was rst investigated (Fig.7a). Aer addition of Tf2O into a solution of amide 1p, the formation of iminium salt Cp (as a 3.4:1 mixture of two geometric isomers) and nitrilium ion Ap in a ratio of Cp:Ap=37:63 (1H NMR, Fig.8a) was observed. The presence of nitrilium ion Ap was manifested by the characteristic triplet resonance and the coupling constant J13C-14N of a nitrilium which appeared at C2=123.4 (t, J13C-14N =45.6Hz54), as well as the nitrilium N- aromatic carbon at C9=121.9

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Figure 6. Mildness of the method and Synthetic applications. (a) Racemization-free synthesis of a versatile chiral building block 3q. (b) Short synthesis of okanin (4).

Figure 7. Proposed mechanisms for the direct CH functionalization of alkenes with secondary amides.

(a) Reaction in the absence of a base. (b) Tf2O/2-uoropyridine-mediated reaction.

(t, J13C-14N =13.5Hz54) (13C NMR, Fig.8b). Besides, the formation of TfOH was also observed by 1H and 13C NMR spectra. The same reaction by bench chemistry produced the enimine 2af in 51% yield along with the recovered

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Figure 8. In situ NMR monitoring of the base-free direct CH functionalization of styrene with secondary amide 1p. (a) 1H NMR spectrum. (b) 13C NMR spectrum.

starting 1p in 31% yield (Fig.7a). Hence, the results obtained from the NMR experiments (Cp:Ap= 37:63) and those from the bench reaction (2af:1p= 38:62) suggested that nitrilium ion Ap was probably the only competent intermediate that reacted with styrene to produce enimine 2af. The less reactive iminium salt Cp was inert to sty-rene addition and hydrolyzed upon work-up to regenerate the starting material 1p. These results also implicated that addition of a base would facilitate the conversion of iminium salt Cp to nitrilium ion Ap, and thus improve the yield of enimine 2af. Experimentally, the addition of 1.2 equiv of 2-uoropyridine boosted the yield of 2af to 97%. In addition, treating a mixture of amide 1p and 2-uoropyridine in CD2Cl2 with Tf2O at 0C resulted in quantitative formation of nitrilium ion intermediate Ap (Fig.7b) along with 2-uoropyridinium salt D within 10 min (cf. Supplementary Figure 57 for NMR spectrum). These results conrmed that 2-uoropyridine promoted the transformation of the iminium salt Cp to nitrilium ion intermediate Ap (Fig.7b). Moreover, the in situ IR monitoring showed the formation of iminium salt Cp (1663cm1) and nitrilium ion Ap (2310cm1) upon

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treatment of amide 1p with Tf2O. The former was converted completely into the latter by action of 2-uoropyridine. A strong absorption of 2-F-pyridinium triuoromethanesulfonate32 (1635cm1) was observed, while no absorption corresponding to pyridinium ion Ep was observed (cf. Supplementary Figure 58 for in situ IR spectra).

Conclusion

In summary, we have developed a general method for the metal-free intermolecular CH functionalization of alkenes with secondary amides. This method provides a direct and high-yielding access to ,-unsaturated ketimines and enones from two classes of readily available and stable starting materials. The one-pot reaction exhibits excellent functional group tolerance for both alkenes and amides allowing convenient and efficient synthesis of a variety of functionalized ,-unsaturated ketimines and enones. The present method could nd wide applications in organic synthesis especially considering the remarkable chemoselectivity.

Methods

General procedure for the direct CH alkyliminylation and acylation of alkenes with secondary amides to give , ,

Into a dry 10-mL round-bottom ask equipped with a magnetic stirring bar were added successively a secondary amide (0.5 mmol, 1.0 equiv), 2 mL of anhydrous CH2Cl2 and 2-uoropyridine (0.6 mmol, 1.2 equiv) under an argon atmosphere. Aer being cooled to 0 C, triuoromethanesulfonic anhydride (Tf2O) (0.55mmol, 1.1 equiv) was added dropwise via a syringe and the reaction was stirred for 10min. To the resulting mixture, an alkene (0.6 mmol, 1.2 equiv) was added dropwise at 0 C. The mixture was allowed to warm-up to room temperature (or 40C) and stirred for 2h. The reaction mixture was concentrated under reduced pressure, and the residue was puried by ash column chromatography (FC) on silica gel (pre-neutralized with 2% Et3N in n-hexane) to aord the desired ,-unsaturated ketimine 2.

Alternately, to the resulting residue were added 5mL of EtOH and 5mL of an aqueous solution of HCl (3.0 M). The resulting mixture was heated to reux until completion of the reaction as monitored by TLC analysis (212h). Aer being cooled to room temperature, 10 mL of CH2Cl2 was added, and the mixture extracted with CH2Cl2 (3 10 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, ltered, and concentrated under reduced pressure. The residue was puried by ash column chromatography on silica gel to aord the desired ,-unsaturated ketone 3.

Data availability. The X-ray crystallographic coordinates for structures reported in this study have been deposited at the Cambridge Crystallographic Date Center (CCDC), under deposition number CCDC 1438540 (for 2w). These data can be obtained free of charge from The Cambridge Crystallographic Data Centre via www.ccdc.cam.ac.uk/data_request/cif.

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Acknowledgements

We are grateful for nancial support from the NSF of China (21332007), the National Basic Research Program (973 Program) of China (Grant No. 2010CB833200), and the Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT) of Ministry of Education, China. We think Professor Ai-Wen Lei (WHU) and Professor Shi-Gang Sun (XMU) for providing their in situ IR facilities, and Mr. Hua-Min Wang (WHU) and Mr. Jian-Yu Ye (XMU) for the help during the IR measurements. We thank Professor Hai-Chao Xu (XMU) for helpful discussion. In memory of the late Professor Wei-Yuan Huang.

Author Contributions

P.-Q.H. conceived, initiated and directed the project, and wrote the manuscript. Y.-H.H. contributed to the conception of the project, carried out the experimental work, and analyzed the data. H.G. contributed, in part, to the experimental work and data analysis. J.-L.Y. contributed to the in situ NMR analysis and analysis of single crystal X-ray data. All authors commented on the manuscript.

Additional Information

Supplementary information accompanies this paper at http://www.nature.com/srep

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www.nature.com/scientificreports/

Competing nancial interests: The authors declare no competing nancial interests.

How to cite this article: Huang, P.-Q. et al. Metal-Free CH Alkyliminylation and Acylation of Alkenes with Secondary Amides. Sci. Rep. 6, 28801; doi: 10.1038/srep28801 (2016).

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