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Abstract
Cissampelos pareira L. var. hirsuta (Buch.-Ham. ex DC.) Forman belongs to family Menispermaceae. The roots of this taxon are used in the treatment of various diseases like stomach pain, fever, skin disease, etc., in Ayurveda and is commonly known as Patha. Two other species, viz., Cyclea peltata (Lam.) Hook.f. & Thomson and Stephania japonica (Thunb.) Miers of the same family are being used as the source of this drug in various parts of India. This type of substitution or adulteration will ultimately affect the therapeutic efficacy of the medicines adversely. ISSR profiles of all the three taxa are generated and analyzed to assess the genetic relationships among these three species. The profiles of all the three species displayed a high level of polymorphism among them. ISSR markers developed can be used in authenticating and validating the exact species discrimination of the genuine raw drug of ‘Patha’ from its substitutes/adulterants to guarantee the quality and legitimacy of this drug in the market.
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Details
1 Eastern Regional Centre, Botanical Survey of India, Shillong, India (GRID:grid.464776.0) (ISNI:0000 0001 0722 6289)
2 Arya Vaidya Sala, Crop Improvement and Biotechnology Division, Centre for Medicinal Plants Research, Malappuram, India (GRID:grid.497494.3) (ISNI:0000 0001 2114 3543)