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Theor Appl Genet (2010) 120:15871596 DOI 10.1007/s00122-010-1278-9
ORIGINAL PAPER
Molecular mapping and characterization of a single dominant gene controlling CMV resistance in peppers (Capsicum annuum L.)
Won-Hee Kang Ngoc Huy Hoang Hee-Bum Yang Jin-Kyung Kwon Sung-Hwan Jo Jang-Kyun Seo Kook-Hyung Kim Doil Choi Byoung-Cheorl Kang
Received: 2 November 2009 / Accepted: 19 January 2010 / Published online: 24 February 2010 Springer-Verlag 2010
Abstract Cucumber mosaic virus (CMV) is one of the most destructive viruses in the Solanaceae family. Simple inheritance of CMV resistance in peppers has not previously been documented; all previous studies have reported that resistance to this virus is mediated by several partially dominant and recessive genes. In this study, we showed that the Capsicum annuum cultivar Bukang contains a single dominant resistance gene against CMVKorean and
CMVFNY strains. We named this resistance gene Cmr1 (Cucumber mosaic resistance 1). Analysis of the cellular localization of CMV using a CMV green Xuorescent protein construct showed that in Bukang, systemic movement of the virus from the epidermal cell layer to mesophyll cells is inhibited. Genetic mapping and FISH analysis revealed that the Cmr1 gene is located at the centromeric region of LG2, a position syntenic to the ToMV resistance locus (Tm-1) in tomatoes. Three SNP markers were developed by
comparative genetic mapping: one intron-based marker using a pepper homolog of Tm-1, and two SNP markers using tomato and pepper BAC sequences mapped near Cmr1. We expect that the SNP markers developed in this study will be useful for developing CMV-resistant cultivars and for Wne mapping the Cmr1 gene.
Introduction
Crop production is constantly challenged by biotic stresses such as fungi, bacteria, and viruses. Viral disease in particular is one of the major problems aVecting pepper production. Cucumber mosaic virus (CMV) was Wrst described in 1916 and is one of the earliest known plant diseases (Jagger 1916). CMV, a member of the Cucumovirus genus in the family Bromoviridae, has the broadest host range of any virus throughout the temperate regions of the world. More than 800 species of plants can be infected by CMV, and more than 60 aphid species are known to be carriers for this virus (Palukaitis et al. 1992). Despite the importance of CMV disease, single dominant resistance genes against CMV in plants...