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Abstract
An in planta gene editing approach was developed wherein Cas9 transgenic plants are infected with an RNA virus that expresses single guide RNAs (sgRNAs). The sgRNAs are augmented with sequences that promote cell-to-cell mobility. Mutant progeny are recovered in the next generation at frequencies ranging from 65 to 100%; up to 30% of progeny derived from plants infected with a virus expressing three sgRNAs have mutations in all three targeted loci.
An efficient and multiplexed in planta gene editing approach is developed by infecting Cas9 transgenic plants with an RNA virus that expresses single guide RNAs carrying sequences that confer cell-to-cell mobility.
Details
Title
Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs
Author
Ellison, Evan E. 1 ; Nagalakshmi, Ugrappa 2 ; Gamo, Maria Elena 3 ; Huang, Pin-jui 2 ; Dinesh-Kumar, Savithramma 2 ; Voytas, Daniel F. 3
1 Department of Genetics, Cell Biology and Development, University of Minnesota, St. Paul, MN, USA (ROR: https://ror.org/017zqws13) (GRID: grid.17635.36) (ISNI: 0000 0004 1936 8657); Center for Precision Plant Genomics, University of Minnesota, St. Paul, MN, USA (ROR: https://ror.org/017zqws13) (GRID: grid.17635.36) (ISNI: 0000 0004 1936 8657); Center for Genome Engineering, University of Minnesota, St. Paul, MN, USA (ROR: https://ror.org/017zqws13) (GRID: grid.17635.36) (ISNI: 0000 0004 1936 8657); Plant and Microbial Biology Graduate Program, University of Minnesota, St. Paul, MN, USA (ROR: https://ror.org/017zqws13) (GRID: grid.17635.36) (ISNI: 0000 0004 1936 8657)
2 Department of Plant Biology and The Genome Center, College of Biological Sciences, University of California, Davis, Davis, CA, USA (ROR: https://ror.org/05rrcem69) (GRID: grid.27860.3b) (ISNI: 0000 0004 1936 9684)
3 Department of Genetics, Cell Biology and Development, University of Minnesota, St. Paul, MN, USA (ROR: https://ror.org/017zqws13) (GRID: grid.17635.36) (ISNI: 0000 0004 1936 8657); Center for Precision Plant Genomics, University of Minnesota, St. Paul, MN, USA (ROR: https://ror.org/017zqws13) (GRID: grid.17635.36) (ISNI: 0000 0004 1936 8657); Center for Genome Engineering, University of Minnesota, St. Paul, MN, USA (ROR: https://ror.org/017zqws13) (GRID: grid.17635.36) (ISNI: 0000 0004 1936 8657)
1 Department of Genetics, Cell Biology and Development, University of Minnesota, St. Paul, MN, USA (ROR: https://ror.org/017zqws13) (GRID: grid.17635.36) (ISNI: 0000 0004 1936 8657); Center for Precision Plant Genomics, University of Minnesota, St. Paul, MN, USA (ROR: https://ror.org/017zqws13) (GRID: grid.17635.36) (ISNI: 0000 0004 1936 8657); Center for Genome Engineering, University of Minnesota, St. Paul, MN, USA (ROR: https://ror.org/017zqws13) (GRID: grid.17635.36) (ISNI: 0000 0004 1936 8657); Plant and Microbial Biology Graduate Program, University of Minnesota, St. Paul, MN, USA (ROR: https://ror.org/017zqws13) (GRID: grid.17635.36) (ISNI: 0000 0004 1936 8657)
2 Department of Plant Biology and The Genome Center, College of Biological Sciences, University of California, Davis, Davis, CA, USA (ROR: https://ror.org/05rrcem69) (GRID: grid.27860.3b) (ISNI: 0000 0004 1936 9684)
3 Department of Genetics, Cell Biology and Development, University of Minnesota, St. Paul, MN, USA (ROR: https://ror.org/017zqws13) (GRID: grid.17635.36) (ISNI: 0000 0004 1936 8657); Center for Precision Plant Genomics, University of Minnesota, St. Paul, MN, USA (ROR: https://ror.org/017zqws13) (GRID: grid.17635.36) (ISNI: 0000 0004 1936 8657); Center for Genome Engineering, University of Minnesota, St. Paul, MN, USA (ROR: https://ror.org/017zqws13) (GRID: grid.17635.36) (ISNI: 0000 0004 1936 8657)
Pages
620-624
Section
Brief Communication
Publication year
2020
Publication date
Jun 2020
e-ISSN
20550278
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
2473192176
Copyright
© The Author(s), under exclusive licence to Springer Nature Limited 2020