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About the Authors:
Charles R. Hesser
Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Validation, Writing - original draft
Affiliation: Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, United States of America
ORCID http://orcid.org/0000-0002-6690-3283
John Karijolich
Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing - review & editing
Affiliations Department of Plant & Microbial Biology, University of California Berkeley, Berkeley, CA, United States of America, Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, United States of America
ORCID http://orcid.org/0000-0002-7925-1122
Dan Dominissini
Roles Data curation, Formal analysis, Methodology, Writing - review & editing
Affiliations Department of Chemistry, Department of Biochemistry and Molecular Biology, and Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois, United States of America, Chaim Sheba Medical Center, Tel Hashomer, Israel, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel
Chuan He
Roles Resources, Writing - review & editing
Affiliations Department of Chemistry, Department of Biochemistry and Molecular Biology, and Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois, United States of America, Howard Hughes Medical Institute, Durham, NC, United States of America
Britt A. Glaunsinger
Roles Conceptualization, Formal analysis, Funding acquisition, Project administration, Supervision, Writing - original draft, Writing - review & editing
* E-mail: [email protected]
Affiliations Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA, United States of America, Department of Plant & Microbial Biology, University of California Berkeley, Berkeley, CA, United States of America, Howard Hughes Medical Institute, Durham, NC, United States of America
ORCID http://orcid.org/0000-0003-0479-9377Abstract
Methylation at the N6 position of adenosine (m6A) is a highly prevalent and reversible modification within eukaryotic mRNAs that has been linked to many stages of RNA processing and fate. Recent studies suggest that m6A deposition and proteins involved in the m6A pathway play a diverse set of roles in either restricting or modulating the lifecycles of select viruses. Here, we report that m6A levels are significantly increased in cells infected with the oncogenic human DNA virus Kaposi’s sarcoma-associated herpesvirus (KSHV). Transcriptome-wide m6A-sequencing of the KSHV-positive renal carcinoma cell line iSLK.219 during lytic reactivation revealed the presence of m6A across multiple kinetic classes of viral transcripts, and a concomitant decrease in m6A levels across much of the host transcriptome....